Amphiphilic glycopolymer nanoparticles as vehicles for nasal delivery of peptides and proteins

Zheng, Chao; Guo, Qianqian; Wu, Zhongming; Sun, Lei; Zhang, Zhengpu; Li, Chaoxing; Zhang, Xinge

doi:10.1016/j.ejps.2013.04.027

Cited by 68 publications

(44 citation statements)

References 39 publications

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“…46 Since insulin contains amino acids, it can form stable complexes with the AAPBA sections of the PAD copolymers through these interactions, 47 in accordance with other reports. 48 PAD-5-1 was loaded with insulin using solutions of varied concentrations, and the results are summarized in Table 4.…”

Section: Insulin Loading and Releasesupporting

confidence: 85%

“…Other studies have also proven that orally administered NPs can effectively lower blood sugar levels in animals, and our results are consistent with those reported by Zhang et al 21 and Sun et al 55 An AAPBA nanogel has additionally been explored for nasal administration. 47 However, treatment of diabetes via oral or nasal administration is problematic because the durations of hypoglycemic efficacy are low, as Guo et al 38 report -typically 12-24 h. Here, we find that we can at least double this time using our novel NPs and IP injection. …”

Section: In Vivo Hypoglycemic Studiesmentioning

confidence: 52%

“…Our results agree well with previously reported studies in this regard. 47,49,54 The insulin-loaded NPs and injection-treated groups have very similar glucose levels from 0 to 48 h after treatment, but after 48 h, the former showed an increase in blood glucose levels. This suggests that the NPs induce a stable hypoglycemic effect in the body over 48 h, but after this time, their efficacy is decreased.…”

Section: In Vivo Hypoglycemic Studiesmentioning

confidence: 90%

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Glucose- and temperature-sensitive nanoparticles for insulin delivery

Wu¹,

Williams²,

Li³

et al. 2017

IJN

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Glucose- and temperature-sensitive polymers of a phenylboronic acid derivative and diethylene glycol dimethacrylate (poly(3-acrylamidophenyl boronic acid- b -diethylene glycol methyl ether methacrylate); p(AAPBA- b -DEGMA)) were prepared by reversible addition–fragmentation chain transfer polymerization. Successful polymerization was evidenced by 1 H nuclear magnetic resonance and infrared spectroscopy, and the polymers were further explored in terms of their glass transition temperatures and by gel permeation chromatography (GPC). The materials were found to be temperature sensitive, with lower critical solution temperatures in the region of 12°C–47°C depending on the monomer ratio used for reaction. The polymers could be self-assembled into nanoparticles (NPs), and the zeta potential and size of these particles were determined as a function of temperature and glucose concentration. Subsequently, the optimum NP formulation was loaded with insulin, and the drug release was studied. We found that insulin was easily encapsulated into the p(AAPBA- b -DEGMA) NPs, with a loading capacity of ~15% and encapsulation efficiency of ~70%. Insulin release could be regulated by changes in temperature and glucose concentration. Furthermore, the NPs were non-toxic both in vitro and in vivo. Finally, the efficacy of the formulations at managing blood glucose levels in a murine hyperglycemic diabetes model was studied. The insulin-loaded NPs could reduce blood glucose levels over an extended period of 48 h. Since they are both temperature and glucose sensitive and offer a sustained-release profile, these systems may comprise potent new formulations for insulin delivery.

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Section: Insulin Loading and Releasesupporting

confidence: 85%

Section: In Vivo Hypoglycemic Studiesmentioning

confidence: 52%

Section: In Vivo Hypoglycemic Studiesmentioning

confidence: 90%

See 1 more Smart Citation

Glucose- and temperature-sensitive nanoparticles for insulin delivery

Wu¹,

Williams²,

Li³

et al. 2017

IJN

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show abstract

“…Insulin-loaded nanoparticles (100 nm) were developed and exhibited strong enzymatic inhibitory activity towards leucine aminopeptidase which triggered enzymatic protein degradation. The nanoparticles maintained the insulin stability for up to 140 h, resulted in higher bioavailability and lowered the blood glucose concentration for 9 h. Similar results were obtained using amphiphilic glycopolymer nanoparticles (Zheng et al, 2013).…”

Section: Further Types Of Nanocarrierssupporting

confidence: 79%

Recent advances in topical delivery of proteins and peptides mediated by soft matter nanocarriers

Witting

Obst

Frieß

et al. 2015

Biotechnology Advances

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“…Their good water solubility, low toxicity and biocompatibility render them a wide range of applications, such as drug delivery [2], vaccines [3], fluorescent probes [4], etc. To further extend these bio-applications to a much boarder area, one feasible method is to hybridize them with inorganic nanomaterials [5,6].…”

Section: Introductionmentioning

confidence: 99%