Amphipathic DNA Origami Nanoparticles to Scaffold and Deform Lipid Membrane Vesicles

Czogalla, Aleksander; Kauert, Dominik J.; Franquelim, Henri G.; Uzunova, Veselina; Zhang, Yixin; Seidel, Ralf; Schwille, Petra

doi:10.1002/anie.201501173

Cited by 110 publications

(137 citation statements)

References 40 publications

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“…13,14 Most recently, bioengineering has focused on DNA nanostructures on the cell surface, resulting in the design of many nanostructures and devices interacting with the cell membrane. 15 Hence, most such DNA nanostructures built on synthetic lipid membranes have served as biomimetic membrane proteins, such as ion channels, 16,17 membrane-sculpting protein 18 and “SNAP (Soluble NSF Attachment Protein) REceptor” (SNARE) protein. 19 To further manipulate DNA nanostructures at the mesoscale, strategies combining dynamic DNA nanotechnologies have been developed to study the complexities of cell membranes, such as cell-surface recognition 20,21 and membrane receptor studies.…”

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confidence: 99%

Facile Assembly/Disassembly of DNA Nanostructures Anchored on Cell-Mimicking Giant Vesicles

et al. 2017

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DNA nanostructures assembled on living cell membranes have become powerful research tools. Synthetic lipid membranes have been used as a membrane model to study the dynamic behavior of DNA nanostructures on fluid soft lipid bilayers, but without the inherent complexity of natural membranes. Herein, we report the assembly and disassembly of DNA nanoprisms on cell-mimicking micrometer-scale giant membrane vesicles derived from living mammalian cells. Three-dimensional DNA nanoprisms with a DNA arm and a cholesterol anchor were efficiently localized on the membrane surface. The assembly and disassembly of DNA nanoprisms were dynamically manipulated by DNA strand hybridization and toehold-mediated strand displacement. Furthermore, the heterogeneity of reversible assembly/disassembly of DNA nanoprisms was monitored by Förster resonance energy transfer. This study suggests the feasibility of DNA-mediated functional biomolecular assembly on cell membranes for biomimetics studies and delivery systems.

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confidence: 99%

Facile Assembly/Disassembly of DNA Nanostructures Anchored on Cell-Mimicking Giant Vesicles

et al. 2017

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“…The second strategy is to reshape the membrane landscape of liposomes with DNA devices that oligomerize or reconfigure on command, which may preserve certain pre-existing membrane features (e.g. lipid composition, internal content) but the end products tend to be less homogeneous 35–37. .…”

Section: Figurementioning

confidence: 99%

Sorting liposomes of distinct sizes by DNA-brick assisted centrifugation

Yang

Wang

et al. 2020

Preprint

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15The "tiny bubbles of fluid" wrapped by lipid-bilayer membranes, termed vesicles, are abundant in 16 cells and extracellular space, performing critical tasks including nutrient uptake, cargo transport, 17 and waste confinement. Vesicles on different missions and transport routes are often distinct both 18 in size and in chemical composition, which confers specificity to their interactions with other 19 membranous compartments. Therefore, to accurately recapitulate the vesicles' structure and 20 behavior, it is important to use homogeneous liposomes (vesicles made of synthetic components) 21 with precisely defined attributes as model membranes. Although existing methods can generate 22

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“…Another amphipathic DNA origami structure consisting of cholesterol anchors on top of a flat membrane binding interface, containing ordered arrays on the membrane due to oligonucleotide overhangs present laterally [29]. Ultimately, this structure is capable of deforming free-standing lipid membranes while showing similar biological activity to coat-forming proteins [30]. Last, amphipathic DNA origami rods with six DNA helices were created along with hydrophobic cholesteryl-ethylene glycol anchors and fluorescently labeled.…”

Section: Applications Of Dna Origamimentioning

confidence: 99%

Beyond the smiley face: applications of structural DNA nanotechnology

Arora

Silva

2018

Nano Reviews & Experiments

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Since the development of DNA origami by Paul Rothemund in 2006, the field of structural DNA nanotechnology has undergone tremendous growth. Through DNA origami and related approaches, self-assembly of specified DNA sequences allows for the ‘bottom-up’ construction of diverse nanostructures. By utilizing different sets of small ‘staple’ DNA strands to direct the folding of a long scaffold strand in diverse ways, DNA origami has particularly been incorporated into a variety of prototypical applications beyond the two-dimensional (2D) smiley face. In this review, the basis of DNA nanotechnology, methods of self-assembly, and Rothemund’s DNA origami breakthrough are discussed first. Next, some of the most promising applications of structural DNA nanotechnology since 2006 are summarized. These include utilizing DNA origami as a tool for creating 3D nanostructures (including DNA bricks), as well as structural (ligand capsid binding, viral capsid binding, DNA NanoOctahedron, DNA mold, photonic devices, energy transfer units), and dynamic (DNA box-with-lid, DNA nano-robot, DNA barges, amphipathic DNA structures, DNA nanocircuits) applications of DNA origami.

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Amphipathic DNA Origami Nanoparticles to Scaffold and Deform Lipid Membrane Vesicles

Cited by 110 publications

References 40 publications

Facile Assembly/Disassembly of DNA Nanostructures Anchored on Cell-Mimicking Giant Vesicles

Facile Assembly/Disassembly of DNA Nanostructures Anchored on Cell-Mimicking Giant Vesicles

Sorting liposomes of distinct sizes by DNA-brick assisted centrifugation

Beyond the smiley face: applications of structural DNA nanotechnology

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