Amphibian Sperm Chromatin Structure and Function and Its Relevance to Sperm Preservation

Pollock, Kate; Gosálvez, Jaime; López‐Fernández, Carmen; Johnston, Stephen D.

doi:10.1670/17-163

Cited by 7 publications

(5 citation statements)

References 62 publications

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“…Successful sperm cryopreservation has now been achieved in many wild species ( Prieto et al, 2014 ) but success has, in many cases, been recognised mainly by the recovery of cellular structure and function (i.e., motility, plasma membrane and acrosomal integrity and, more recently, the retention of functional mitochondria and undamaged DNA ( Paoli et al, 2019 ; Pollock et al, 2018 ). While these parameters are informative, they do not necessarily guarantee that spermatozoa will have the capacity to both fertilize oocytes and support appropriate embryonic development.…”

Section: Integration Of Biobanks and Biodiversity Conservationmentioning

confidence: 99%

Biobanks, offspring fitness and the influence of developmental plasticity in conservation biology

Holt

2023

Anim. Reprod.

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Mitigation of the widely known threats to the world's biodiversity is difficult, despite the strategies and actions proposed by international agreements such as the United Nations Framework Convention on Climate Change (UNFCCC) and the Convention on Biological Diversity (CBD). Nevertheless, many scientists devote their time and effort to finding and implementing various solutions to the problem. One potential way forward that is gaining popularity involves the establishment of biobank programs aimed at preserving and storing germplasm from threatened species, and then using it to support the future viability and health of threatened populations. This involves developing and using assisted reproductive technologies to achieve their goals. Despite considerable advances in the effectiveness of reproductive technologies, differences between the reproductive behavior and physiology of widely differing taxonomic groups mean that this approach cannot be applied with equal success to many species. Moreover, evidence that epigenetic influences and developmental plasticity, whereby it is now understood that embryonic development, and subsequent health in later life, can be affected by peri-conceptional environmental conditions, is raising the possibility that cryopreservation methods themselves may have to be reviewed and revised when planning the biobanks. Here, I describe the benefits and problems associated with germplasm biobanking across various species, but also offer some realistic assessments of current progress and applications.

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Section: Integration Of Biobanks and Biodiversity Conservationmentioning

confidence: 99%

Biobanks, offspring fitness and the influence of developmental plasticity in conservation biology

Holt

2023

Anim. Reprod.

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“…In humans, males are considered infertile when sperm viability falls below 75 -80% sperm regardless of whether sperm are motile [85]. Therefore, assessments that do not include these metrics could be underestimating male infertility [86]. For external fertilizers such as A. boreas boreas, viability could be an important measure of the longevity of inheritable genetic material when implementing amphibian ARTs especially if the induction of sperm is to be used to genetically manage or for long-term storage of the species' genetic diversity.…”

Section: Hormone Protocol and Ebp Reproductive Managementmentioning

confidence: 99%

Hormonal induction, quality assessments and the influence of seasonality on male reproductive viability in a long-term managedex situbreeding colony of Southern Rocky Mountain Boreal toads,Anaxyrus boreas boreas

Calatayud,

Jacobs,

Togna

et al. 2023

Preprint

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The Southern Rocky Mountain Boreal Toad (Anaxyrus boreas boreas) is an ex situ managed species which relies heavily on the use of assisted reproductive technologies to augment existing in situ populations. Despite the use of ARTs to manipulate reproduction of this species, the ex situ program continues to face challenges with annual reproduction. Human chorionic gonadotropin (hCG) at 10 IU/gbw singly or in combination with 0.4 ug/gbw GnRH-A have been successfully reported for this species, however, with a number of other available hormones, it is not clear if these are the most effective protocols for this species. Moreover, there is no information on how exogenous hormone administration is affected by other factors such as seasonality. Therefore, in the first part of this study, we compare the efficacy of the 10 IU/g hCG and 0.4 𝜇g/g Gonadotropin Releasing Hormone agonist (GnRH-A) administered singly or in combination, as well as GnRH-Apd + Amphiplex (0.4 𝜇g/g GnRH-A + 10 𝜇g/g Metoclopramide [MET] - a dopamine antagonist), or 10 𝜇g/g MET alone. Spermiation responses and sperm viability were compared across treatments with hormonal curves then correlated to seasonality. The results of this study suggest that the optimal hormonal stimulation protocol, across all treatments, in terms of sperm quality was 10 IU/g hCG + 0.4 𝜇g/g GnRH-A. Further optimization is required, in particular the exploration of higher doses of GnRH-A. Lastly, we observed that the effect of seasonality influenced the hormonal efficacy and magnitude of the spermiation response. As was expected, quality and concentration parameters were affected by the month in which hormone administration occurred.

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“…Does the incidence of sperm DNA fragmentation really indicate a quality, or is it just a biological trait? Although knowledge about sperm DNA fragmentation is limited in amphibians, recent reports show that the incidence in fresh sperm cells is higher than in other taxa (17,68). Repair mechanisms might be available in the oocyte after fertilization, like in humans (69).…”

Section: Semen Collection and Evaluationmentioning

confidence: 99%

Recent Progress in Spermatology Contributing to the Knowledge and Conservation of Rare and Endangered Species

Comizzoli

Holt

2022

Annu. Rev. Anim. Biosci.

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There is a remarkable diversity in the animal kingdom regarding mechanisms underlying the production, maturation, structure, and function of sperm cells. Spermatology studies contribute to the knowledge of species diversity and also provide information about individual or population fitness. Furthermore, this fundamental research is required before collected spermatozoa can be used for conservation breeding, including assisted reproduction and cryobanking. This article aims to ( a) review the most recent knowledge on sperm morphology and function in wild animal species, ( b) analyze how this knowledge can be used to save species in their natural habitat or ex situ, and ( c) propose future scientific directions in wildlife spermatology that could positively impact animal conservation. Variations in sperm structure and performance within and between species have multiple origins and significance. This collective body of knowledge enables the design and implementation of conservation strategies and action plans that integrate several disciplines. Expected final online publication date for the Annual Review of Animal Biosciences, Volume 10 is February 2022. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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Amphibian Sperm Chromatin Structure and Function and Its Relevance to Sperm Preservation

Cited by 7 publications

References 62 publications

Biobanks, offspring fitness and the influence of developmental plasticity in conservation biology

Biobanks, offspring fitness and the influence of developmental plasticity in conservation biology

Hormonal induction, quality assessments and the influence of seasonality on male reproductive viability in a long-term managedex situbreeding colony of Southern Rocky Mountain Boreal toads,Anaxyrus boreas boreas

Recent Progress in Spermatology Contributing to the Knowledge and Conservation of Rare and Endangered Species

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