Amphibian pore-forming protein βγ-CAT drives extracellular nutrient scavenging under cell nutrient deficiency

Liu, Ling‐Zhen; Liu, Long; Shi, Zhi-Hong; Bian, Xian-Ling; Si, Ziru; Wang, Qiquan; Xiang, Yang; Zhang, Yun

doi:10.1016/j.isci.2023.106598

Cited by 1 publication

(4 citation statements)

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“…In addition to playing an important anti‐microbial role, our recent studies also showed that the B. maxima af‐PFP complex βγ‐CAT can promote tissue repair, 44 help toads maintain water balance under osmotic stress, 45 and acquire or deliver nutrients under starvation conditions 14,15 . These findings suggested that af‐PFPs may play multiple roles in toads depending on environmental conditions.…”

Section: Discussionmentioning

confidence: 87%

“…In addition to playing an important anti-microbial role, our recent studies also showed that the B. maxima af-PFP complex βγ-CAT can promote tissue repair, 44 help toads maintain water balance under osmotic stress, 45 and acquire or deliver nutrients under starvation conditions. 14,15 These findings suggested that af-PFPs may play multiple roles in toads depending on environmental conditions. Similarly, transcriptomic analysis of LIN-24 RNAi and LIN-24-OE worms showed that LIN-24 is involved in pathways other than those associated with the immune response, such as longevity regulation, fatty acid degradation, membrane transport, oxidoreductase regulation, and metabolism (Figures 2 and 6).…”

Section: Discussionmentioning

confidence: 92%

“…Furthermore, af‐PFPs seem to be involved in important physiological functions. Ep37 has been suggested to be related to newt development, 4 and we recently found that βγ‐CAT helps protect animals and cells against metabolic stresses such as starvation 14,15 . Overexpression of a flower‐specific af‐PFP from the dioecious plant Rumex acetosa alters flower development and induces male sterility in transgenic tobacco 16 .…”

Section: Introductionmentioning

confidence: 93%

“…Ep37 has been suggested to be related to newt development, 4 and we recently found that βγ-CAT helps protect animals and cells against metabolic stresses such as starvation. 14,15 Overexpression of a flower-specific af-PFP from the dioecious plant Rumex acetosa alters flower development and induces male sterility in transgenic tobacco. 16 However, due to the existence of numerous isoforms within the same species and lack of specific tools such as antibodies or probes, 11 the physiological roles of af-PFPs are difficult to systematically investigate in these species.…”

Section: Introductionmentioning

confidence: 99%

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Caenorhabditis elegansLIN‐24, a homolog of bacterial pore‐forming toxin, protects the host from microbial infection

Zhang,

Zeng,

Zhao

et al. 2023

The FASEB Journal

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Aerolysin‐like pore‐forming protein (af‐PFP) superfamily members are double‐edge swords that assist the bacterial infection but shied bacteria from the host by various mechanisms in some species including the toad Bombina maxima and zebrafish. While members of this family are widely expressed in all kingdoms, especially non‐bacteria species, it remains unclear whether their anti‐bacterial function is conserved. LIN‐24 is an af‐PFP that is constitutively expressed throughout the Caenorhabditis elegans lifespan. Here, we observed that LIN‐24 knockdown reduced the maximum lifespan of worms. RNA‐seq analysis identified 323 differentially expressed genes (DEGs) post‐LIN‐24 knockdown that were enriched in “immune response” and “lysosome pathway,” suggesting a possible role for LIN‐24 in resisting microbial infection. In line with this, we found that Pseudomonas aeruginosa 14 (PA14) infection induced LIN‐24 expression, and that survival after PA14 infection was significantly reduced by LIN‐24 knockdown. In contrast, LIN‐24 overexpression (LIN‐24‐OE) conferred protection against PA14 infection, with worms showing longer survival time and reduced bacterial load. Weighted gene co‐expression network analysis of LIN‐24‐OE worms showed that the highest correlation module was enriched in factors related to immunity and the defense response. Finally, by predicting transcription factors from RNA‐seq data and knocking down candidate transcription factors in LIN‐24‐OE worms, we revealed that LIN‐24 may protect worms against bacterial infection by stimulating DAF‐16‐mediated immune responses. These findings agree with our previous studies showing an anti‐microbial role for the amphibian‐derived af‐PFP complex βγ‐CAT, suggesting that af‐PFPs may play a conserved role in combatting microbial infections. Further research is needed to determine the roles this protein family plays in other physio‐pathological processes, such as metabolism, longevity, and aging.

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Section: Discussionmentioning

confidence: 87%