Amperometric trienzyme ATP biosensors based on the coimmobilization of salicylate hydroxylase, glucose-6-phosphate dehydrogenase, and hexokinase

Cui, Yue; Barford, J. P.; Renneberg, Reinhard

doi:10.1016/j.snb.2008.01.001

Cited by 27 publications

(10 citation statements)

References 24 publications

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“…[273–275] In one case, ATP, adenosine diphosphate (ADP), and adenosine monophosphate (AMP) were all detected at the same untreated electrode. [276] In common with biosensors for other neurotransmitters, rejection of interfering species, improved biocompatibility, and enhanced ease of fabrication were several motivations for new ATP biosensors.…”

Section: Nucleosidesmentioning

confidence: 99%

“…[269] presented a technique for more simple preparation of ATP biosensors. A pair of groups sought shorter measurement periods for biosensors,[273, 274] including real-time monitoring. [274] Mizaikoff and colleagues[270, 271] developed and tested a generalized theory for the preparation and optimization of dual enzyme biosensors so that future biosensors could be developed more rapidly.…”

Section: Nucleosidesmentioning

confidence: 99%

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Review of recent advances in analytical techniques for the determination of neurotransmitters

Perry

Kennedy

2009

Analytica Chimica Acta

330

221

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Methods and advances for monitoring neurotransmitters in vivo or for tissue analysis of neurotransmitters over the last five years are reviewed. The review is organized primarily by neurotransmitter type. Transmitter and related compounds may be monitored by either in vivo sampling coupled to analytical methods or implanted sensors. Sampling is primarily performed using microdialysis, but low-flow push-pull perfusion may offer advantages of spatial resolution while minimizing the tissue disruption associated with higher flow rates. Analytical techniques coupled to these sampling methods include liquid chromatography, capillary electrophoresis, enzyme assays, sensors, and mass spectrometry. Methods for the detection of amino acid, monoamine, neuropeptide, acetylcholine, nucleoside, and soluable gas neurotransmitters have been developed and improved upon. Advances in the speed and sensitivity of these methods have enabled improvements in temporal resolution and increased the number of compounds detectable. Similar advances have enabled improved detection at tissue samples, with a substantial emphasis on single cell and other small samples. Sensors provide excellent temporal and spatial resolution for in vivo monitoring. Advances in application to catecholamines, indoleamines, and amino acids have been prominent. Improvements in stability, sensitivity, and selectivity of the sensors have been of paramount interest.

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Section: Nucleosidesmentioning

confidence: 99%

Section: Nucleosidesmentioning

confidence: 99%

Review of recent advances in analytical techniques for the determination of neurotransmitters

Perry

Kennedy

2009

Analytica Chimica Acta

330

221

View full text Add to dashboard Cite

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“…Owing to its high catalytic activity and substrate specificity, G6PD has been extensively used as reagent in varied enzymatic assays aiming determinations of ATP and hexose concentrations [5]. Besides, using G6PD in enzyme-based biosensor would be a good option due to advantages as specific measurement of target analyte in complex matrices (e.g., blood, food products, and environmental samples) [6,7]. However, the potential application of G6PD requires an efficient strategy to its recovery from remaining yeast cells of fermentation medium and, although particularly abundant in Saccharomyces cerevisiae, a baker or brewer yeast, C. guilliermondii could be a feasible option of this enzyme source.…”

Section: Introductionmentioning

confidence: 99%

Process Integration for the Disruption of Candida guilliermondii Cultivated in Rice Straw Hydrolysate and Recovery of Glucose-6-Phosphate Dehydrogenase by Aqueous Two-Phase Systems

Gurpilhares

Pessoa

Roberto

2015

Appl Biochem Biotechnol

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Remaining cells of Candida guilliermondii cultivated in hemicellulose-based fermentation medium were used as intracellular protein source. Recovery of glucose-6-phosphate dehydrogenase (G6PD) was attained in conventional aqueous two-phase systems (ATPS) was compared with integrated process involving mechanical disruption of cells followed by ATPS. Influences of polyethylene glycol molar mass (M PEG) and tie line lengths (TLL) on purification factor (PF), yields in top (Y T ) and bottom (Y B ) phases and partition coefficient (K) were evaluated. First scheme resulted in 65.9 % enzyme yield and PF of 2.16 in salt-enriched phase with clarified homogenate (M PEG 1500 g mol(-1), TLL 40 %); Y B of 75.2 % and PF B of 2.9 with unclarified homogenate (M PEG 1000 g mol(-1), TLL 35 %). The highest PF value of integrated process was 2.26 in bottom phase (M PEG 1500 g mol(-1), TLL 40 %). In order to optimize this response, a quadratic model was predicted for the response PFB for process integration. Maximum response achieved was PFB = 3.3 (M PEG 1500 g mol(-1), TLL 40 %). Enzyme characterization showed G6P Michaelis-Menten constant (K M ) equal 0.07-0.05, NADP(+) K M 0.02-1.98 and optimum temperature 70 °C, before and after recovery. Overall, our data confirmed feasibility of disruption/extraction integration for single-step purification of intracellular proteins from remaining yeast cells.

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“…In this paper, we present a spectroscopic method based on a competitive enzymatic reaction between hexokinase (HEX) and glucose oxidase (GOX) for the detection of ATP. Sun et al reported an assay to detect ATP via a competitive enzymatic reaction, hexokinase (HEX)/glucose oxidase (GOX) [22][23][24]. Additionally, various researchers have developed a glucose sensor using quantum dots.…”

Section: Introductionmentioning

confidence: 99%

InP/ZnS Nanocrystals as Fluorescent Probes for the Detection of ATP

Massadeh

Nann

2014

Nanomaterials and Nanotechnology

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This article reports on a study on fluorescence adenosine triphosphate (ATP) detection by InP/ZnS quantum dots (QDs). We present a spectroscopic analysis displaying the effect of enzymatic reactions of glucose oxidase (GOX) and hexokinase (HEX) on the InP/ZnS quantum dots at physiological pH. The InP/ZnS quantum dots act as glucose sensors in the presence of GOX, Glu and ATP, and their luminescence quenches during the release of hydrogen peroxide from the reaction. However, in the presence of adenosine 5' triphosphate, glucose, and HEX, a significant photobrightening of the InP/ZnS QDs is recorded. This is dependent on the concentration of ATP in the sample. The relationship between the ATP and the emission intensity of InP/ZnS nanocrystals is linear. The present results are the first to report the effect of different byproducts released by these enzymatic reactions on the fluorescence of the InP/ZnS QDs.

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Amperometric trienzyme ATP biosensors based on the coimmobilization of salicylate hydroxylase, glucose-6-phosphate dehydrogenase, and hexokinase

Cited by 27 publications

References 24 publications

Review of recent advances in analytical techniques for the determination of neurotransmitters

Review of recent advances in analytical techniques for the determination of neurotransmitters

Process Integration for the Disruption of Candida guilliermondii Cultivated in Rice Straw Hydrolysate and Recovery of Glucose-6-Phosphate Dehydrogenase by Aqueous Two-Phase Systems

InP/ZnS Nanocrystals as Fluorescent Probes for the Detection of ATP

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