“…In the latter case, this type of biosensor is called "biosensor based on enzyme inhibition". The first biosensor based on cholinesterase (ChE) inhibition for detection of nerve agents was developed by G.Guilbaut in 1962 [1] and from this one, a lot of ChE biosensors were developed for several compounds such as heavy metals [2,3], organophosphorus and carbammic insecticides [4][5][6][7][8], toxins [9,10], glycoalkaloids [11,12], drugs [13][14][15], fluoride [16,17], cocaine [18,19] and nicotine [20,21]. In details, during the last 9 years more than 100 papers were published on the ChE biosensors.…”