Amperometric mediated carbon paste biosensor based on D-fructose dehydrogenase for the determination of fructose in food analysis

Paredes, Pedro A.; Parellada, Josefina; Fernández, Vı́ctor M.; Katakis, Ioanis; Domı́nguez, Elena

doi:10.1016/s0956-5663(97)00090-0

Cited by 59 publications

(31 citation statements)

References 25 publications

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“…The design of the bioelectrode is based on a MPAmodified AuE on which TTF and fructose dehydrogenase (FDH) are co-immobilized by cross-linking. Fructose is frequently used as a dietetic sweetener, and its determination is of interest in the food industry, for example, in the detection of honey adulteration or in the follow-up of fruit ripening [7]. Although several classical methods can be used for the determination of fructose [8,9], fast, lowcost, selective methodologies suitable to be applied in a simple manner are required in the food industry for quality control.…”

Section: Introductionmentioning

confidence: 99%

“…Re-oxidation of H 2 PQQ may be achieved directly or indirectly on electrodes giving a current proportional to the fructose concentration [7]. Although several examples of direct electrocatalysis, in which electrons are directly transferred to the electrode, can be found in the literature [10,11,12,13], the relatively high operation potential needed, as well as the low current densities achieved, led us to use an indirect electrocatalytic design in which a mediator is employed as the electron acceptor.…”

Section: Introductionmentioning

confidence: 99%

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An integrated electrochemical fructose biosensor based on tetrathiafulvalene-modified self-assembled monolayers on gold electrodes

Campuzano

Gálvez²,

Pedrero

et al. 2003

Analytical and Bioanalytical Chemistry

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An integrated amperometric fructose biosensor based on a gold electrode (AuE) modified with a self-assembled monolayer (SAM) of 3-mercaptopropionic acid (MPA) on which fructose dehydrogenase (FDH) and the mediator tetrathiafulvalene (TTF) are co-immobilized by cross-linking with glutaraldehyde is reported. Variables concerning the behavior of the biosensor were optimized by taking the slope value obtained for the fructose calibration plot in the 0.1-1.0 mM concentration range as a criterion of selection.At an applied potential of +0.20 V, a good repeatability of such slope values (RSD=6.7%, n=10) was obtained with no need to apply a cleaning or pretreatment procedure to the modified electrode. Moreover, results from five different TTF-FDH-MPA-AuEs yielded a RSD of 5.8%. The useful lifetime of one single biosensor was approximately 30 days, exhibiting a 93% of the original response on the 33rd day. A linear calibration graph was obtained for fructose over the 1.0×10 -5 -1.0×10 -3 M range, with a limit of detection of 2.4×10 -6 M. The effect of potential interferents was evaluated. The TTF-FDH-MPA-AuE also performed well in the flow-injection mode. The biosensor was used for the determination of fructose in real samples, and the results compared with those provided by using a commercial enzyme test kit.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An integrated electrochemical fructose biosensor based on tetrathiafulvalene-modified self-assembled monolayers on gold electrodes

Campuzano

Gálvez²,

Pedrero

et al. 2003

Analytical and Bioanalytical Chemistry

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“…[57,58]. Oxidoreductases were used to detect glucose [59], fructose [60], amino acids [61], and lactates [62]. Transferases were used in biosensoric analysis of atrazine [63], and hydrolases to determine saccharose [64].…”

Section: Enzymes Used In Biosensorsmentioning

confidence: 99%

Enzyme-Based Fluorescent Biosensors and Their Environmental, Clinical and Industrial Applications

Kłos-Witkowska¹

2015

Pol. J. Environ. Stud.

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The last two decades has seen a significant increase in interest in biosensors. According to the Web of Science database, the number of scientific publications over the past 20 years increased from 309 in 1993 up to 3,467 in 2013 (Fig. 1).The demand for developing new detection methods, increasing interest in visualization techniques [1][2][3], emphasis placed on new drug discovery programs [4], diagnostic test development [5][6][7], and attempts to explain cellular action mechanisms and to trace a cell's metabolism pathway [7,8], and the possibility of using biosensors in numerous fields of application, (for example: medicine [9-11], environmental protection [12-14, 16-17], food industry [18], and defense industry [19]) has results in an increasing number of research projects targeted at biosensor design and fabrication. Biosensors used in environmental monitoring measure the toxicity effect based on the detection of a chemical compound or compound group by selective recognition of a biomolecule in the receptor layer and then by detection of a signal after passing through the transducer layer [16].The application of fluorescence biosensors in environmental protection applications [20][21][22][23][24][25], medical diagnostics [26][27][28][29][30][31][32][33][34][35], and industries [36][37][38][39][40][41][42][43][44][45] also is growing.Pol. J. Environ. Stud. Vol. 24, No. 1 (2015) AbstractEnzyme-based fluorescence biosensors and their applications in environmental protection, medicine, and industry are described. Biosensors used in environmental protection measure toxicity effects. A chemical compound or group of compounds is detected by the recognition of molecules in the receptor layer and then by detecting a signal passing through the transducer layer. Biosensors are classified according to the transduction method. Special emphasis is placed on optical biosensors, especially fluorescent biosensors, and such measurement techniques as FRET (Fröster resonance energy transfer), FLIM (fluorescence lifetime imaging), FCS (fluorescence correlation spectroscopy), and changes in fluorescence intensity. The phenomenon of fluorescence in biosensors and the selection of appropriate methods are described. The use of enzymes in the receptor layer and enzyme classification according to its category and functions used for analyte detection are presented. The fluorescence properties of enzymes resulting from possessing such cofactors as flavin or heme (prosthetic) groups are discussed. Several methods for enzyme immobilization, namely entrapment, adsorption, covalent immobilization, cross linking, and affinity interaction are described, and the use of enzymatic fluorescence biosensors in the detection of analytes is presented.

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“…당 성분을 측정하기 위한 방법으로는 크로마토그래 피, [1][2][3] 염료의 색 변화를 이용하여 정량하는 비색법, [4][5][6] 효소를 이용한 전기화학 바이오센서, [7][8][9] 효소 없이 탄소 을 수정하여 전기도금을 수행하였다. 전기도 금법은 10 …”

Section: 서 론unclassified

A Strip Sensor Based on PbO₂/Carbon Paste Electrode to Determine Sweetener Contents in Fruits

Lee¹,

Cho²,

Heo³

et al. 2014

Journal of the Korean Electrochemical Society

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: A strip sensor based on PbO 2 /carbon paste electrode was prepared by a screen-printing method, and employed to electrochemically determine the concentration of fruit sweeteners(i.e. glucose, sucrose, and fructose). The PbO 2 /carbon paste electrode could monitor electrocatalytic oxidation of organic compounds such as carbohydrates, and measure the levels of natural sweeteners without enzyme. Severe interference from ascorbic acid was effectively reduced by modifying the electrode surface with a Nafion membrane. The response level of the Nafion/PbO 2 /carbon paste electrode increased in the order of fructose, sucrose, and glucose, which corresponds to the order of sweetness perceived by humans.

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Amperometric mediated carbon paste biosensor based on D-fructose dehydrogenase for the determination of fructose in food analysis

Cited by 59 publications

References 25 publications

An integrated electrochemical fructose biosensor based on tetrathiafulvalene-modified self-assembled monolayers on gold electrodes

An integrated electrochemical fructose biosensor based on tetrathiafulvalene-modified self-assembled monolayers on gold electrodes

Enzyme-Based Fluorescent Biosensors and Their Environmental, Clinical and Industrial Applications

A Strip Sensor Based on PbO₂/Carbon Paste Electrode to Determine Sweetener Contents in Fruits

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Amperometric mediated carbon paste biosensor based on D-fructose dehydrogenase for the determination of fructose in food analysis

Cited by 59 publications

References 25 publications

An integrated electrochemical fructose biosensor based on tetrathiafulvalene-modified self-assembled monolayers on gold electrodes

An integrated electrochemical fructose biosensor based on tetrathiafulvalene-modified self-assembled monolayers on gold electrodes

Enzyme-Based Fluorescent Biosensors and Their Environmental, Clinical and Industrial Applications

A Strip Sensor Based on PbO2/Carbon Paste Electrode to Determine Sweetener Contents in Fruits

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A Strip Sensor Based on PbO₂/Carbon Paste Electrode to Determine Sweetener Contents in Fruits