Amperometric immunosensor for the determination of IgA deficiency in human serum samples

Rosales-Rivera, Luis Carlos; Acero-Sánchez, Josep Lluís; Lozano-Sánchez, Pablo; Katakis, Ioanis; O’Sullivan, Ciara K.

doi:10.1016/j.bios.2011.12.040

Cited by 22 publications

(8 citation statements)

References 35 publications

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“…Cleaned gold biochips and stock solutions of the bipodal alkanethiol (DT2) in ethanol were prepared as described elsewhere . The gold slides were immersed in 2 m m DT2 in ethanol for 3 h at room temperature (RT) to form a S‐S‐polyethylene glycol (PEG)‐COOH self‐assembled monolayer, then rinsed with ethanol, and dried by nitrogen.…”

Section: Methodsmentioning

confidence: 99%

S‐S‐PEG‐COOH Self‐Assembled Monolayer on Gold Surface Enabled a Combined Assay for Serological EBV Antibody Isotypes

Liu

et al. 2018

Proteomics Clinical Apps

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Purpose: Epstein-Barr virus (EBV) is a ubiquitous human gamma herpes virus that infects human epithelial cells and B lymphocytes. It would be potentially valuable to develop novel combined assays to benefit screening for large panels of samples of EBV infectious diseases. Experimental design: A simple antigen-probed biochip that is modified with S-S-PEG-COOH and is used as a label-free high-throughput screening method for a combined detection of EBV capsid antigen IgM antibody, capsid antigen IgG antibody, and nuclear antigen IgG antibody.Results: This protein biochip has similar feasibility, sensitivity, and specificity in comparison with Liaison chemiluminescent immunoassay (CLIA). Detection limit of the EBV antibodies by the biochip is almost identical to that by CLIA-L (2.91 U mL -1 vs 3.00 U mL -1 for EBNA-1 IgG, 8 U mL -1 vs10 U mL -1 for EBV-VCA IgG, and 3.5 U mL -1 vs 10 U mL -1 for EBV-VCA IgM). Tests of the three serological antibodies against EBV by the biochip are consistent with the CLIA-L method in 274 clinical sera, respectively. Finally, the combined biochip is successfully utilized for diagnostic identification of EBV infection in 14 patients with infectious mononucleosis (IM) and 25 patients with systemic lupus erythematosus SLE, as well as additional 10 known real-time PCR positive patients.

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Section: Methodsmentioning

confidence: 99%

S‐S‐PEG‐COOH Self‐Assembled Monolayer on Gold Surface Enabled a Combined Assay for Serological EBV Antibody Isotypes

Liu

et al. 2018

Proteomics Clinical Apps

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“…Similarly, also for serum samples, dilution is the most often utilized technique and is combined with additional processing steps, such as centrifugation for dopamine [ 48 ], uric acid [ 48 ], glucose [ 49 ], and immunoglobulin A [ 62 ] analysis; precipitation for dopamine [ 53 ] and biphenyl [ 58 ] analysis; deproteinization with acid and filtration for glucose [ 55 ] analysis. It is important to keep in mind, though, that in some instances, especially in single-use devices, biosensors are described that can deal with the complex blood matrix without sample pretreatment step such as shown for glucose where Nafion membranes are known to cut down the most prevalent interferences such as ascorbic and uric acid [ 63 ] and nucleic acids (miRNAs) [ 64 ].…”

Section: Pairing (Electrochemical) Biosensors With Sample Preparationmentioning

confidence: 99%

“…Similar to blood, saliva samples suffer from an immense component complexity and variation of compositions. Here, filtration and dilution methods are for example utilized for lactate [ 73 ] and nitrite/nitrate [ 62 ] analysis, respectively.…”

Section: Pairing (Electrochemical) Biosensors With Sample Preparationmentioning

confidence: 99%

Combining Electrochemical Sensors with Miniaturized Sample Preparation for Rapid Detection in Clinical Samples

Bunyakul

Baeumner

2014

Sensors

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Clinical analyses benefit world-wide from rapid and reliable diagnostics tests. New tests are sought with greatest demand not only for new analytes, but also to reduce costs, complexity and lengthy analysis times of current techniques. Among the myriad of possibilities available today to develop new test systems, amperometric biosensors are prominent players—best represented by the ubiquitous amperometric-based glucose sensors. Electrochemical approaches in general require little and often enough only simple hardware components, are rugged and yet provide low limits of detection. They thus offer many of the desirable attributes for point-of-care/point-of-need tests. This review focuses on investigating the important integration of sample preparation with (primarily electrochemical) biosensors. Sample clean up requirements, miniaturized sample preparation strategies, and their potential integration with sensors will be discussed, focusing on clinical sample analyses.

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“…They have many advantages including simple instrumentation and operation, high sensitivity and selectivity, and wide linear range. Since enzyme labels provide great signal amplification in the assay and also a large number of antibody-enzyme or antigen-enzyme conjugates are commercially available, the majority of electrochemical immunoassays are based on the use of specific enzyme/substrate couples [103][104][105]. Sensitivities of ng/ml can routinely be achieved in enzyme-based electrochemical immunosensors.…”

Section: Sensitivitymentioning

confidence: 99%

Electroanalytical Sensor Technology

Power¹,

Morrin²

2013

Electrochemistry

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Amperometric immunosensor for the determination of IgA deficiency in human serum samples

Cited by 22 publications

References 35 publications

S‐S‐PEG‐COOH Self‐Assembled Monolayer on Gold Surface Enabled a Combined Assay for Serological EBV Antibody Isotypes

S‐S‐PEG‐COOH Self‐Assembled Monolayer on Gold Surface Enabled a Combined Assay for Serological EBV Antibody Isotypes

Combining Electrochemical Sensors with Miniaturized Sample Preparation for Rapid Detection in Clinical Samples

Electroanalytical Sensor Technology

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