Amperometric immunosensor developed for sensitive detection of SARS-CoV-2 spike S1 protein in combined with portable device

Erdem, Arzum; Senturk, Huseyin; Yildiz, Esma; Maral, Meltem

doi:10.1016/j.talanta.2022.123422

Cited by 29 publications

(20 citation statements)

References 46 publications

(74 reference statements)

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“…The LOD was reported for the surface plasmon resonance-based aptasensor (0.26 nM), 39 lateral flow immunoassay (LFIA) (>0.1 ng/mL), 40 , 41 the organic field effect transistor (OFET) (76.61 pg/mL), 42 nanomechanical sensor-based platform (1 ng/mL), 43 electrochemical immunosensor (19 ng/mL), 44 glycan-based lateral flow detection system (5 μg/mL), 45 photoelectrochemical aptasensor (72 ng/mL), 46 nanobody-based ELISA (0.147 ng/mL), 47 and portable amperometric immunosensor (0.15 ng/mL). 48 Apparently, the method we developed exhibits a similar LOD as the OFET 42 but outperforms most methods in sensitivity for the detection of spike antigens. The good sensitivity contributes to the screened phage #2 expressing the NFWISPKLAFAL peptide from the display library of the pIII phage, which has an excellent affinity to SARS-CoV-2 S1.…”

Section: Resultsmentioning

confidence: 85%

Discovery of a Phage Peptide Specifically Binding to the SARS-CoV-2 Spike S1 Protein for the Sensitive Phage-Based Enzyme-Linked Chemiluminescence Immunoassay of the SARS-CoV-2 Antigen

Liu

et al. 2022

Anal. Chem.

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The COVID-19 pandemic has led to a global crisis with devastating effects on public healthcare and the economy. Sensitive detection of SARS-CoV-2 is the key to diagnose and control its spread. The spike (S) protein is an abundant viral transmembrane protein and a suitable target protein for the selective recognition of SARS-CoV-2. Here, we report that with bovine serum albumin prescreening, a specific phage peptide targeting SARS-CoV-2 S1 protein was biopanned with the pIII phage display library. The identified phage #2 expressing the peptide (amino acid sequence: NFWISPKLAFAL) shows high affinity to the target with a dissociation constant of 3.45 ± 0.58 nM. Furthermore, the identified peptide shows good specificity with a binding site at the N-terminal domain of the S1 subunit through a hydrogen bond network and hydrophobic interaction, supported by molecular docking. Then, a sandwiched phage-based enzyme-linked chemiluminescence immunoassay (ELCLIA) was established by using phage #2 as a bifunctional probe capable of SARS-CoV-2 S1 antigen recognition and signal amplification. After optimizing the conditions, the proposed phage ELCLIA exhibited good sensitivity, and as low as 78 pg/mL SARS-CoV-2 S1 could be detected. This method can be applied to detect as low as 60 transducing units (TU)/mL SARS-CoV-2 pseudovirus in 50% saliva. Therefore, specific phage peptides have good prospects as powerful biological recognition probes for immunoassay detection and biomedical applications.

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Section: Resultsmentioning

confidence: 85%

Discovery of a Phage Peptide Specifically Binding to the SARS-CoV-2 Spike S1 Protein for the Sensitive Phage-Based Enzyme-Linked Chemiluminescence Immunoassay of the SARS-CoV-2 Antigen

Liu

et al. 2022

Anal. Chem.

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“…There have been many reports in the literature presenting the detection of SARS-CoV-2 virus by different procedures in combination with specific bioreceptors, such as spike protein (S) and nucleocapsid protein (N) [ 5 , 47 , 48 ]. In addition to the studies targeting spike protein [ 4 , 6 , 7 , 10 , 27 , 49 , 50 ], some studies focusing on the detection of S1 subunits [ 12 , 51 , 52 ] are also presented. The detection of the SARS-CoV-2 virus using combinations of electrochemical techniques, such as voltametric [ 4 , 47 , 48 , 49 , 51 , 53 , 54 ], amperometric [ 5 , 9 , 52 ] and impedimetric [ 6 , 7 , 10 , 27 , 55 ] techniques, was explored for the determination of different bioreceptors of SARS-CoV-2.…”

Section: Resultsmentioning

confidence: 99%

“…Activation of the surface of the SPE: Electrochemical activation of the electrode surface was performed similarly to a previous study [ 12 ]. The solution containing 5 mM EDC and 8 mM NHS was prepared in PBS (50 mM, pH 7.40), as reported in earlier literature [ 38 , 39 ].…”

Section: Methodsmentioning

confidence: 99%

“…At this time, the diagnosis of the SARS-CoV-2 virus is of key importance due to the effects of the COVID-19 pandemic on all over the world, and reports on the topic [ 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ] are increasingly available in the literature while COVID-19 pandemic still continues.…”

Section: Introductionmentioning

confidence: 99%

“…Nowadays, SPEs are more preferentially used, due to advantages such as disposability and greater suitability for implementation to portable devices, being able to provide sensitive and precise analysis using a small of sample [ 12 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Impedimetric Detection Based on Label-Free Immunoassay Developed for Targeting Spike S1 Protein of SARS-CoV-2

et al. 2022

Self Cite

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After the COVID-19 pandemic started all over the world, great importance was placed on the development of sensitive and selective bioanalytical assays for the rapid detection of the highly pathogenic SARS-CoV-2 virus causing COVID-19 disease. In this present work, an impedimetric immunosensor was developed and applied for rapid, reliable, sensitive and selective detection of the SARS-CoV-2 S1 protein. To detect the SARS-CoV-2 virus, targeting of the spike S1 protein was achieved herein by using S1 protein-specific capture antibody (Cab-S1) immobilized screen-printed electrode (SPE) in combination with the electrochemical impedance spectroscopy (EIS) technique. With the impedimetric immunosensor, the detection limit for S1 protein in buffer medium was found to be 0.23 ng/mL (equal to 23.92 amol in 8 µL sample) in the linear concentration range of S1 protein from 0.5 to 10 ng/mL. In the artificial saliva medium, it was found to be 0.09 ng/mL (equals to 9.36 amol in 8 µL sample) in the linear concentration range of S1 protein between 0.1 and 1 ng/mL. The selectivity of the impedimetric immunosensor toward S1 protein was tested against influenza hemagglutinin antigen (HA) in the buffer medium as well as in artificial saliva.

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Highly Reusable Electrochemical Immunosensor for Ultrasensitive Protein Detection

Singampalli,

Neal – Harris,

Yee

et al. 2024

Advanced Sensor Research

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The detection and quantification of protein biomarkers in bodily fluids is important for many clinical applications, including disease diagnosis and health monitoring. Current techniques for ultrasensitive protein detection, such as enzyme‐linked immunosorbent assay (ELISA) and electrochemical sensing, involve long incubation times (1.5–3 h) and rely on single‐use sensing electrodes which can be costly and generate excessive waste. This work demonstrates a reusable electrochemical immunosensor employing magnetic nanoparticles (MNPs) and dually labeled gold nanoparticles (AuNPs) for ultrasensitive measurements of protein biomarkers. As proof of concept, this platform is used to detect C‐X‐C motif chemokine ligand 9 (CXCL9), a biomarker associated with kidney transplant rejection, immune nephritis from checkpoint inhibitor therapy, and drug‐associated acute interstitial nephritis, in human urine. The sensor successfully detects CXCL9 at concentrations as low as 27 pg mL−1 within ≈1 h. This immunosensor was also adapted onto a handheld smartphone‐based diagnostic device and used for measurements of CXCL9, which exhibited a lower limit of detection of 65 pg mL−1. Lastly, this work demonstrates that the sensing electrodes can be reused for at least 100 measurements with a negligible loss in analytical performance, reducing the costs and waste associated with electrochemical sensing.

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Amperometric immunosensor developed for sensitive detection of SARS-CoV-2 spike S1 protein in combined with portable device

Cited by 29 publications

References 46 publications

Discovery of a Phage Peptide Specifically Binding to the SARS-CoV-2 Spike S1 Protein for the Sensitive Phage-Based Enzyme-Linked Chemiluminescence Immunoassay of the SARS-CoV-2 Antigen

Discovery of a Phage Peptide Specifically Binding to the SARS-CoV-2 Spike S1 Protein for the Sensitive Phage-Based Enzyme-Linked Chemiluminescence Immunoassay of the SARS-CoV-2 Antigen

Impedimetric Detection Based on Label-Free Immunoassay Developed for Targeting Spike S1 Protein of SARS-CoV-2

Highly Reusable Electrochemical Immunosensor for Ultrasensitive Protein Detection

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