Amperometric Glucose Sensors for Whole Blood Measurement Based on Dehydrogenase Enzymes

Cardosi, Marco F.; Liu, Zuifang

doi:10.5772/48491

Cited by 15 publications

(11 citation statements)

References 77 publications

(70 reference statements)

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“…Secondly, NAD + , can adsorb onto the electrode surface. Both of these processes are irreversible and produce a gradual passivation of the electrode during continued biosensor operation [ 83 ].…”

Section: Enzyme Biosensor Analytical Performance Over Timementioning

confidence: 99%

Enzyme Biosensors for Biomedical Applications: Strategies for Safeguarding Analytical Performances in Biological Fluids

Rocchitta

Spanu

Babudieri

et al. 2016

Sensors

408

264

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Enzyme-based chemical biosensors are based on biological recognition. In order to operate, the enzymes must be available to catalyze a specific biochemical reaction and be stable under the normal operating conditions of the biosensor. Design of biosensors is based on knowledge about the target analyte, as well as the complexity of the matrix in which the analyte has to be quantified. This article reviews the problems resulting from the interaction of enzyme-based amperometric biosensors with complex biological matrices containing the target analyte(s). One of the most challenging disadvantages of amperometric enzyme-based biosensor detection is signal reduction from fouling agents and interference from chemicals present in the sample matrix. This article, therefore, investigates the principles of functioning of enzymatic biosensors, their analytical performance over time and the strategies used to optimize their performance. Moreover, the composition of biological fluids as a function of their interaction with biosensing will be presented.

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Section: Enzyme Biosensor Analytical Performance Over Timementioning

confidence: 99%

Enzyme Biosensors for Biomedical Applications: Strategies for Safeguarding Analytical Performances in Biological Fluids

Rocchitta

Spanu

Babudieri

et al. 2016

Sensors

408

264

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“…Although DI from Bacillus stearothermophilus and NAD-GDH are insensitive to oxygen [12,22], the redox reaction between Os(bpy) 2 Cl 2 and dissolved O 2 can influence the rate of ENN redox cycling. However, metal complexes such as Os(bpy) 2 Cl 2 mainly undergo outer-sphere reactions and they react more slowly with dissolved O 2 than organic electron mediators do.…”

Section: Effect Of Dissolved O 2 and Specificity Of The Sensormentioning

confidence: 99%

Specific and Rapid Glucose Detection Using NAD‐dependent Glucose Dehydrogenase, Diaphorase, and Osmium Complex

2019

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Selective glucose measurement in serum and blood and rapid glucose measurement using nicotinamide adenine dinucleotide (NAD)‐dependent glucose dehydrogenase (NAD‐GDH) are still very challenging. Here, we report a selective and rapid glucose sensor, based on electrochemical‐enzymatic‐enzymatic (ENN) redox cycling involving bis(2,2‐bipyridyl)dichloroosmium(II) [Os(bpy)2Cl2], diaphorase (DI), NAD+, NAD‐GDH, and glucose. DI and Os(bpy)2Cl2 are used to obtain fast mediated oxidation of NADH that is generated as a result of glucose oxidation by NAD‐GDH. DI and NAD‐GDH are co‐immobilized via affinity binding on an avidin‐modified indium tin oxide electrode to obtain fast and stable ENN redox cycling. Two enzymes (DI and NAD‐GDH) and two electron mediators [Os(bpy)2Cl2 and NAD+] are insensitive to oxygen. The applied potential (0.0 V vs Ag/AgCl) is low enough to minimize interfering electrochemical reactions, and the redox reactions of Os(bpy)2Cl2 with interfering species are slow. NAD‐GDH is much less reactive to problematic monosaccharides such as xylose, fructose, galactose, and mannose than glucose. Artificial serum containing 5 % (w/v) human serum albumin shows a similar electrochemical background level in serum. All results enable us to obtain selective and reproducible glucose detection. The fast ENN redox cycling allows sensitive glucose detection with a wide range of concentrations in artificial serum with a short measuring time (5 s) without an incubation period.

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“…The constant biasing potential acts as the stimulus that drives the output current of the test strip. The details of the potentials required for different test strips were obtained from the literature [5][6][7][8] and verified by independent characterisation.…”

Section: B Potentiostatmentioning

confidence: 99%

Simulation of a multi-strip blood glucometer

Anoop

Mohan

Guruvayurappan

2014

TENCON 2014 - 2014 IEEE Region 10 Conference

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Normal blood glucose levels are tightly regulated in the human body, in the range of 70-150 mg/dL. With the increasing awareness that uncontrolled diabetes plays a significant role in the onset and progress of other diseases, severely diabetic patients are being advised to closely monitor their haemoglucose levels, as often as four times every day. This is done in a home environment, by means of a glucometer and enzyme-coated test strips, having high specificity for glucose. The test strips are expensive and specific to the glucometer of a particular manufacturer, which implies that a patient is tied to one glucometer, making it economically unviable, especially in a developing country like India. Efforts are being made to develop a portable glucometer, which would accommodate, if not all, most of the commercial glucose test strips, available in the market today. As a first step, three of the most widely used test strips have been characterised and a circuit has been designed to obtain their current responses. This paper describes the simulation of the circuit and discusses the results obtained.

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Amperometric Glucose Sensors for Whole Blood Measurement Based on Dehydrogenase Enzymes

Cited by 15 publications

References 77 publications

Enzyme Biosensors for Biomedical Applications: Strategies for Safeguarding Analytical Performances in Biological Fluids

Enzyme Biosensors for Biomedical Applications: Strategies for Safeguarding Analytical Performances in Biological Fluids

Specific and Rapid Glucose Detection Using NAD‐dependent Glucose Dehydrogenase, Diaphorase, and Osmium Complex

Simulation of a multi-strip blood glucometer

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