Amperometric flow-injection determination of sucrose with a mediated tri-enzyme electrode based on sucrose phosphorylase and electrocatalytic oxidation of NADH

Maestre, Estrella; Katakis, Ioanis; Domı́nguez, Elena

doi:10.1016/s0956-5663(00)00139-1

Cited by 28 publications

(8 citation statements)

References 30 publications

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“…At-site sampling is probably more suited to liquids (e.g., beer, wine, and fruit juices) where (generally) the target analyte of interest is more readily available. Similarly, sampling from fresh produce is usually not as challenging as for other more heterogeneous foodstuffs as many of the potential target analytes are in solution when tissue is disrupted/decompartmentalized and, thus, available for measurement in extracted fruit juice (11,28,29,46). This said, significant variation in the spatial and temporal distribution of target analytes does exist in all fresh produce; a fact that reconfirms that sampling procedures must be optimized.…”

Section: Biosensor Optimization For Food Analysismentioning

confidence: 99%

The Application of Biosensors to Fresh Produce and the Wider Food Industry

Terry

White

Tigwell

2005

J. Agric. Food Chem.

258

128

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The inherent specificity, selectivity, and adaptability of biosensors make them ideal candidates for use throughout the food industry. Potential applications within the supply chain range from testing of foodstuffs for maximum pesticide residue verification through to the routine analysis of analyte(s) concentrations, such as, glucose, sucrose, alcohol, etc., which may be indicators of food quality/acceptability. Biosensor formats include simple "one-shot" disposable devices that can be used either in the field or integrated into more sophisticated laboratory instruments. Until now, the main impact of these devices has been in the medical diagnostics field. However, with ongoing technical development, the food industry will be one of the prime beneficiaries of biosensor technology in the future. This report assesses the current and future trends in the application of biosensors to fresh produce and the wider food industry, focusing on both potential and current target analytes that are fundamental to fresh produce quality, traceability, and safety.

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Section: Biosensor Optimization For Food Analysismentioning

confidence: 99%

The Application of Biosensors to Fresh Produce and the Wider Food Industry

Terry

White

Tigwell

2005

J. Agric. Food Chem.

258

128

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“…As mentioned previously, all catalytic reactions are based on NAD + /NADH dependent dehydrogenases, with the exception of fructose dehydrogenase which is a PQQ (pyrroloquinoline quinone) dependent enzyme (Matsumoto et al, 1988). The transduction chemistry of all these reactions is based on osmium complexes and their electrochemical behaviour has been previously described (Hedenmo et al, 1996;Paredes et al, 1997;Popescu et al, 1999;Maestre et al, 2001). It is worth noting that the electrocatalytic oxidation of NADH is mediated by 1,10-phenanthroline-5,6-dione stabilised by complex formation with Os (4,4 -dimethyl 2,2 -bipyridine) 2 as ligand.…”

Section: Application Of the Mafec To The Simultaneous Determination Omentioning

confidence: 94%

“…In the case of sucrose determination, the interference of glucose can be suppressed by a catalytic route based on sucrose phosphorylase (Kogure et al, 1997;Maestre et al, 2001). The pH profile, however, of this enzyme shows low activity at pH 5 (Maestre et al, 2001). Consequently and in search of simultaneous analysis including All the experiments were performed in 0.1 M acetate buffer pH 5.…”

Section: Application Of the Mafec To The Simultaneous Determination Omentioning

confidence: 99%

“…The analytical performance of the cell is demonstrated through the simultaneous bioelectrocatalytic detection of five sugars in food samples using dehydrogenase-based carbon paste electrodes. Our previous transducing schemes for the selective detection of mono (Hedenmo et al, 1996;Paredes et al, 1997) and disaccharides (Maestre et al, 2001) are incorporated into the multianalyte flow electrochemical system. It will be shown that after calibration of the system and following the injection of a single sample, five sugars can be simultaneously quantified in food samples that have not been pre-treated.…”

Section: Introductionmentioning

confidence: 99%

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A multianalyte flow electrochemical cell: application to the simultaneous determination of carbohydrates based on bioelectrocatalytic detection

Maestre

Katakis

Narváez

et al. 2005

Biosensors and Bioelectronics

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“…In this section, we review the main characteristics of molecular receptors, natural and synthetic, capable of selectively binding sucrose. Enzymes and proteins include: invertase (INV) [ 68 , 69 , 70 , 71 , 72 , 73 , 74 ], sucrose phosphorylase (SP) [ 75 , 76 ], Fluorescent Indicator Proteins (FLIP) [ 14 , 90 ], and lectins [ 91 , 92 , 93 , 94 , 95 ].…”

Section: Sucrose Quantification Using Molecular Recognition Methodsmentioning

confidence: 99%

Molecular Level Sucrose Quantification: A Critical Review

Lara-Cruz

Jaramillo-Botero

2022

Sensors

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Sucrose is a primary metabolite in plants, a source of energy, a source of carbon atoms for growth and development, and a regulator of biochemical processes. Most of the traditional analytical chemistry methods for sucrose quantification in plants require sample treatment (with consequent tissue destruction) and complex facilities, that do not allow real-time sucrose quantification at ultra-low concentrations (nM to pM range) under in vivo conditions, limiting our understanding of sucrose roles in plant physiology across different plant tissues and cellular compartments. Some of the above-mentioned problems may be circumvented with the use of bio-compatible ligands for molecular recognition of sucrose. Nevertheless, problems such as the signal-noise ratio, stability, and selectivity are some of the main challenges limiting the use of molecular recognition methods for the in vivo quantification of sucrose. In this review, we provide a critical analysis of the existing analytical chemistry tools, biosensors, and synthetic ligands, for sucrose quantification and discuss the most promising paths to improve upon its limits of detection. Our goal is to highlight the criteria design need for real-time, in vivo, highly sensitive and selective sucrose sensing capabilities to enable further our understanding of living organisms, the development of new plant breeding strategies for increased crop productivity and sustainability, and ultimately to contribute to the overarching need for food security.

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Amperometric flow-injection determination of sucrose with a mediated tri-enzyme electrode based on sucrose phosphorylase and electrocatalytic oxidation of NADH

Cited by 28 publications

References 30 publications

The Application of Biosensors to Fresh Produce and the Wider Food Industry

The Application of Biosensors to Fresh Produce and the Wider Food Industry

A multianalyte flow electrochemical cell: application to the simultaneous determination of carbohydrates based on bioelectrocatalytic detection

Molecular Level Sucrose Quantification: A Critical Review

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