Amperometric flow-injection determination of phenolic compounds at self-assembled monolayer-based tyrosinase biosensors

Campuzano, Susana; Serra, B.; Pedrero, Marı́a; Villena, F. Javier Manuel de; Pingarrón, José M.

doi:10.1016/s0003-2670(03)00919-x

Cited by 139 publications

(76 citation statements)

References 23 publications

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“…Sensitivity corresponding to the linear range for catechol was 51.58 mA mM -1 , which is higher than that obtained on Horseradish peroxidase biosensor and tyrosinase biosensors-modified electrodes. [35][36][37] Moreover, the detection limit of catechol was found to be about 1.4 mM according to the formula 3s b /m criteria, which is lower than that obtained in previous report, 38,39 demonstrating that Au/TiO 2 -BDD3 electrode is a better choice for catechol detection.…”

Section: Optimization Of the Experimental Conditionsmentioning

confidence: 87%

Electrochemical Detection of Catechol on Boron‐doped Diamond Electrode Modified with Au/TiO₂ Nanorod Composite

Wei

Liu

et al. 2011

J Chinese Chemical Soc

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Au/TiO 2 nanorod composites with different ratios of [TiO 2 ]: [Au] have been prepared by chemically reducing AuCl 4 -on the positively charged TiO 2 nanorods surface and used to modify boron-doped diamond (BDD) electrodes. The electrochemical behaviors of catechol on the bare and different Au/TiO 2 nanorod composites-modified BDD electrodes are studied. The cyclic voltammetric results indicate that these different Au/TiO 2 nanorod composites-modified BDD electrodes can enhance the electrocatalytic activity toward catechol detection, as compared with the bare BDD electrode. Among these different conditions, the Au/TiO 2 -BDD3 electrode (the ratio of [TiO 2 ]:[Au] is 27:1) is the most choice for catechol detection. The electrochemical response dependences of the Au/TiO 2 -BDD3 electrode on pH of solution and the applied potential are studied. The detection limit of catechol is found to be about 1.4 × 10 -6 M in a linear range from 5 × 10 -6 M to 200 × 10 -6 M on the Au/TiO 2 -BDD3 electrode.

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Section: Optimization Of the Experimental Conditionsmentioning

confidence: 87%

Electrochemical Detection of Catechol on Boron‐doped Diamond Electrode Modified with Au/TiO₂ Nanorod Composite

Wei

Liu

et al. 2011

J Chinese Chemical Soc

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“…Amperometric methods mainly use electrochemical sensors and biosensors as detectors in FIA. Tyrosinase (Tyr) based sensors have been the most widely used amperometric biosensor for determination of phenols and were prepared in a number of matrices and conventional electrodes, including carbon paste [13], conducting polymers [14,15], CNT conducting polymer composites [16,17], self-assembled monolayer [18], and silica sol-gel composite [19]. Laccase based biosensors [20,21] and horseradish peroxidase (HRP) [22] based electrochemical detectors coupled with flow injection system for the determination of phenol and phenol derivatives were also reported.…”

Section: Isrn Analytical Chemistrymentioning

confidence: 99%

Determination of Phenol and Chlorophenols at Single-Wall Carbon Nanotubes/Poly(3,4-ethylenedioxythiophene) Modified Glassy Carbon Electrode Using Flow Injection Amperometry

Negash

Alemu

Tessema

2014

ISRN Analytical Chemistry

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Phenol and chlorophenols were investigated using single-wall carbon nanotubes (SWCNT) and poly(3,4-ethylenedioxythiophene) (PEDOT) composite modified glassy carbon electrode (SWCNT/PEDOT/GCE) as a detector in flow injection system. Optimization of experimental variables such as the detection potential, flow rate, and pH of the carrier solution (0.1 M sodium acetate) for the determination of phenol (P), 4-chlorophenol (CP), 2,4-dichlorophenol (DCP), 2,4,6-trichlorophenol (TCP), and pentachlorophenol (PCP) were performed. Under these conditions, analytical parameters were calculated from the calibration curve of measured amperometric responses as a function of concentrations of phenol and chlorophenols. The designed electrode exhibited very good analytical performance. The designed electrode was tested with 20 repetitive injections of each analyte and showed good operational stability. The analytical performance of the SWCNT/PEDOT/GCE electrode under flow through conditions was tested and was found to be impressive. The electrode showed a wider dynamic range for the detection of phenol and chlorophenols with low limits of detection compared with other enzymatic and nonenzymatic sensors. These results suggest that the method is quite useful for the analysis and monitoring of phenols and chlorophenols.

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“…Electrochemical biosensors based on tyrosinase (Tyr) or polyphenol oxidase enzymes are con-sidered as an alternative to the conventional techniques due to their simplified sample treatment, and the possibility of portable, economic, fast, and sensitive analysis [5][6][7]. Several research groups have investigated Tyr-based bio-sensors for the detection of phenols [8][9][10]. Tyrosinasecatalysed oxidation of tyrosine and other monohydric phenols involves o-hydroxylation followed by oxidation of the resulting dihydric phenol to the corresponding o-qui-none in a single step without the release of the dihydric phenol intermediate.…”

Section: Introductionmentioning

confidence: 99%

“…A key factor in the con-struction of a biosensor is the need to achieve adequate and effective enzyme immobilization. Some of the common approaches that have been used for the immobilization of Tyr onto various substrates include carbon paste immobi-lization [9,13,14], sol-gel immobilization [15], physical adsorption [16] and electrochemical entrapment of the enzyme within a polymer or a composite matrix [17]. However, some of these methods are relatively complex.…”

Section: Introductionmentioning

confidence: 99%

Electroanalysis of urinary l-dopa using tyrosinase immobilized on gold nanoelectrode ensembles

Pinho

Subramanian²,

Ribeiro³

et al. 2012

J Appl Electrochem

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The performance of an amperometric biosensor constructed by associating tyrosinase (Tyr) enzyme with the advantages of a 3D gold nanoelectrode ensemble (GNEE) is evaluated in a flowinjection analysis (FIA) system for the analysis of Levodopa (Ldopa). GNEEs were fabricated by electroless deposition of the metal within the pores of polycarbonate track-etched membranes. A simple solvent etching procedure based on the solubility of polycarbonate membranes is adopted for the fabrication of the 3D GNEE. Afterwards, enzyme was immobilized onto preformed self-assembled monolayers of cysteamine on the 3D GNEEs (GNEE-Tyr) via cross-linking with glutaraldehyde. The experimental conditions of the FIA system, such as the detection potential (-0.200 V (vs. Ag/ AgCl)) and flow rates (1.0 mL min -1 ) were optimized. Analytical responses for L-dopa were obtained in a wide concentration range between 1 9 10 -8 mol L -1 and 1 9 10 -2 mol L -1 . The limit of quantification was found to be 1 9 10 -8 mol L -1 with a resultant % RSD of 7.23% (n = 5). The limit of detection was found to be 1 9 10 -9 mol L -1 (S/N = 3). The common interfering compounds namely, glucose (10 mmol L -1 ), ascorbic acid (10 mmol L -1 ), and urea (10 mmol L -1 ) were studied. The recovery of L-dopa (1 9 10 -7 mol L -1 ) from spiked urine samples was found to be 96%. Therefore, the developed method is adequate to be applied in the clinical analysis.

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Amperometric flow-injection determination of phenolic compounds at self-assembled monolayer-based tyrosinase biosensors

Cited by 139 publications

References 23 publications

Electrochemical Detection of Catechol on Boron‐doped Diamond Electrode Modified with Au/TiO₂ Nanorod Composite

Electrochemical Detection of Catechol on Boron‐doped Diamond Electrode Modified with Au/TiO₂ Nanorod Composite

Determination of Phenol and Chlorophenols at Single-Wall Carbon Nanotubes/Poly(3,4-ethylenedioxythiophene) Modified Glassy Carbon Electrode Using Flow Injection Amperometry

Electroanalysis of urinary l-dopa using tyrosinase immobilized on gold nanoelectrode ensembles

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Amperometric flow-injection determination of phenolic compounds at self-assembled monolayer-based tyrosinase biosensors

Cited by 139 publications

References 23 publications

Electrochemical Detection of Catechol on Boron‐doped Diamond Electrode Modified with Au/TiO2 Nanorod Composite

Electrochemical Detection of Catechol on Boron‐doped Diamond Electrode Modified with Au/TiO2 Nanorod Composite

Determination of Phenol and Chlorophenols at Single-Wall Carbon Nanotubes/Poly(3,4-ethylenedioxythiophene) Modified Glassy Carbon Electrode Using Flow Injection Amperometry

Electroanalysis of urinary l-dopa using tyrosinase immobilized on gold nanoelectrode ensembles

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Electrochemical Detection of Catechol on Boron‐doped Diamond Electrode Modified with Au/TiO₂ Nanorod Composite

Electrochemical Detection of Catechol on Boron‐doped Diamond Electrode Modified with Au/TiO₂ Nanorod Composite