Amperometric Flow‐Injection Determination of Phenolic Compounds Using a Biosensor with Immobilized Laccase, Peroxidase and Tyrosinase

Solná, Renáta; Skládal, Petr

doi:10.1002/elan.200403343

Cited by 64 publications

(30 citation statements)

References 47 publications

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“…The limit of detection was determined to be 3.9 nM (S/N=3). This value is in the range of previously published papers with different enzymes [125][126][127][128][129][130].…”

Section: Peroxide-dependent Oxidation Of P-aminophenol and Anilinesupporting

confidence: 52%

Can peroxygenase and microperoxidase substitute cytochrome P450 in biosensors

Yarman

Peng

et al. 2011

Bioanal Rev

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Aromatic peroxygenase (APO) from the basidiomycetous mushroom Agrocybe aegerita (AaeAPO) and microperoxidases (MPs) obtained from cytochrome c exhibit a broad substrate spectrum including hydroxylation of selected aromatic substrates, demethylation and epoxidation by means of hydrogen peroxide. It overlaps with that of cytochrome P450 (P450), making MPs and APOs to alternate recognition elements in biosensors for the detection of typical P450 substrates. Here, we discuss recently developed approaches using microperoxidases and peroxygenases in view of their potential to supplement P450 enzymes as recognition elements in biosensors for aromatic compounds. Starting as early as the 1970s, the direct electron transfer between electrodes and the heme group of heme peptides called microperoxidases has been used as a model of oxidoreductases. These MP-modified electrodes are used as hydrogen peroxide detectors based on the catalytic current generated by electrically contacted microperoxidase molecules. A similar catalytic reaction has been obtained for the electrode-immobilised heme protein AaeAPO. However, up to now, no MP-based sensors for substrates have been described. In this review, we present biosensors which indicate 4-nitrophenol, aniline, naphthalene and p-aminophenol based on the peroxide-dependent substrate conversion by electrode-immobilised MP and AaeAPO. In these enzyme electrodes, the signal is generated by the conversion of all substrates, thus representing in complex media an overall parameter. The performance of these sensors and their further development are discussed in comparison with P450-based electrodes

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“…The limit of detection was determined to be 3.9 nM (S/N=3). This value is in the range of previously published papers with different enzymes [125][126][127][128][129][130].…”

Section: Peroxide-dependent Oxidation Of P-aminophenol and Anilinesupporting

confidence: 52%

Can peroxygenase and microperoxidase substitute cytochrome P450 in biosensors

Yarman

Peng

et al. 2011

Bioanal Rev

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“…The system was successfully applied to the simultaneous determination of glucose and ascorbic acid in an attempt to determine simultaneously glucose plus its typical interferents in biological fluids without any separation stage or barrier membrane. In a very interesting study case [93], Solna and Skladal attempted the determination of various phenolic compounds combining the responses of a multiple screen-printed amperometric enzyme biosensor employing laccase, tyrosinase and peroxidase enzymes. The amperometric flow-injection determination of phenolic compounds at discrete applied potentials and using the multichannel biosensor was demonstrated at the nM level, although the multivariate approach with combination of the multiple responses was not fully developed.…”

Section: Electronic Tongues Employing Biosensorsmentioning

confidence: 99%

Electronic Tongues Employing Electrochemical Sensors

2010

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This review presents recent advances concerning work with electronic tongues employing electroanalytical sensors. This new concept in the electroanalysis sensor field entails the use of chemical sensor arrays coupled with chemometric processing tools, as a mean to improve sensors performance. The revision is organized according to the electroanalytical technique used for transduction, namely: potentiometry, voltammetry/amperometry or electrochemical impedance. The significant use of biosensors, mainly enzyme-based is also presented. Salient applications in real problem solving using electrochemical electronic tongues are commented.

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“…In an interesting work by Skladal, a four-electrode biosensor based on immobilization of laccase (Coriolus hirsutus), peroxidase (horseradish) and tyrosinase (mushroom) in a same screen-printed array was developed for generic monitoring of phenols [57]. The enzymes were immobilized onto a self-assembled monolayer (4-mercapto-1-butanol)-modified gold surface via covalent attachment by epichlorohydrin coupling.…”

Section: Determining Several Substratesmentioning

confidence: 99%

Bioelectronic Tongues Employing Electrochemical Biosensors

Valle

2016

Bioanalytical Reviews

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This chapter presents recent advances concerning work with electronic tongues that employ electrochemical biosensors, that is, bioelectronic tongues. (Bio)electronic tongues represent a new methodological use of (bio)sensors; they start by the use of biosensor arrays and assume the coupling of the obtained complex response with advanced chemometric data treatment; the goal is improving performance of existing sensors. Most of the bioelectronic tongues reported employ enzyme biosensors, essentially based on potentiometric or voltammetric/ amperometric transduction. This report is organized considering the different forms to incorporate biosensors, i.e. considering the number of biosensors in the array, the number of different enzymes used, if the determination is aimed to substrates or inhibitors, etc. Significant applications in real problem-solving, mainly in the food and clinical or environmental fields, are commented.

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Amperometric Flow‐Injection Determination of Phenolic Compounds Using a Biosensor with Immobilized Laccase, Peroxidase and Tyrosinase

Cited by 64 publications

References 47 publications

Can peroxygenase and microperoxidase substitute cytochrome P450 in biosensors

Can peroxygenase and microperoxidase substitute cytochrome P450 in biosensors

Electronic Tongues Employing Electrochemical Sensors

Bioelectronic Tongues Employing Electrochemical Biosensors

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