Amperometric Determination of Hydrogen Peroxide and its Mathematical Simulation for Horseradish Peroxidase Immobilized on a Sonogel Carbon Electrode

Bensana, Amira; Achi, Fethi; Bouguettoucha, Abdallah; Chebli, Derradji

doi:10.1080/00032719.2018.1528614

Cited by 6 publications

(1 citation statement)

References 55 publications

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“…The cumulative release of HRP was monitored timely using ultraviolet spectrophotometer at 403 nm. Furthermore, the concentration change of hydrogen peroxide in the presence of HRP‐loaded microcapsules was measured using electrochemical methods (Bensana, Achi, Bouguettoucha, & Chebli, 2018). In the method, ketjen black and chitosan were used as conductive agent and binder, respectively, both substances were used to modify electrode together with HRP.…”

Section: Methodsmentioning

confidence: 99%

Reactive oxygen species‐responsive silk sericin microcapsules used for antioxidative stress damage

Wang

Sun

et al. 2020

Microscopy Res & Technique

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Silk sericin microcapsules loaded with horseradish peroxidase (HRP) are prepared through protein self-assembly in a green environment containing enzymes to protect liver cells from alcohol damage. Load content and release dynamics of HRP in sericin microcapsules are investigated. The role of HRP-loaded microcapsules in hydrogen peroxide (H 2 O 2) degradation is demonstrated using electrochemical method. Furthermore, the effect of the HRP-loaded microcapsules on cells and intracellular reactive oxygen species (ROS) level is evaluated using an alcohol damage model in vitro. Results show that HRP can be loaded effectively in the sericin microcapsules and can be released ROS-responsively from microcapsules. Cell survival rate increases after suffering from alcohol damage due to the presence of HRP-loaded microcapsule, and the active oxygen content in cells is maintained at a stable level even when it remained in an environment with high alcohol concentration. We believe that the internalized sericin microcapsules maintain HRP activity intracellularly, allow controlled HRP release within a host cell, and show excellent ability in antioxidative stress injury.

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Section: Methodsmentioning

confidence: 99%

Reactive oxygen species‐responsive silk sericin microcapsules used for antioxidative stress damage

Wang

Sun

et al. 2020

Microscopy Res & Technique

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Eco-friendly spark-generated CoxOy nanoparticle-modified graphite screen-printed sensing surfaces for the determination of H2O2 in energy drinks

Siampani,

Lazanas,

Spyrou

et al. 2024

Microchim Acta

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The modification of graphite screen-printed electrodes (SPEs) is reported using an eco-friendly and extremely fast method based on the direct cobalt pin electrode-to-SPE spark discharge at ambient conditions. This approach does not utilize any liquids or chemical templates, does not produce any waste, and allows the in-situ generation of CoxOy nanoparticles onto the electrode surface and the development of efficient electrocatalytic sensing surfaces for the determination of H2O2. Co-spark SPEs were characterized using scanning electron microscopy, energy-dispersive X-ray spectroscopy and x-ray photoelectron spectroscopy (XPS), revealing the formation of surface confined CoxOy nanoparticles and the diverse oxidation states of cobalt species. Co-spark SPEs were also characterized with cyclic voltammetry and electrochemical impedance spectroscopy. Redox transitions of the surface confined electrocatalysts are demonstrated by electrochemical polarization studies, showing the formation of different oxides (CoxOy), varying the XPS results. Amperometric measurements at 0.3 V vs. Ag/AgCl revealed a linear relationship between the current response and the concentration of H2O2 over the range 1 − 102 μM, achieving a limit of detection (3σ/m) of 0.6 μM. The interference effect of various electroactive species was effectively addressed by employing dual measurements in the absence and presence of the enzyme catalase. The analytical utility of the method was evaluated in antioxidant rich real-world samples, such as energy drinks, demonstrating sufficient recovery. Graphical Abstract

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Electrochemical Detection of Lead in Real Samples Using Carbon Nanostructure and Inactivated E. coli as Low-Cost Sensitive Biosensor with High Electrocatalytic Performance

Sabah

Achi

2022

Electrocatalysis

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Amperometric Determination of Hydrogen Peroxide and its Mathematical Simulation for Horseradish Peroxidase Immobilized on a Sonogel Carbon Electrode

Cited by 6 publications

References 55 publications

Reactive oxygen species‐responsive silk sericin microcapsules used for antioxidative stress damage

Reactive oxygen species‐responsive silk sericin microcapsules used for antioxidative stress damage

Eco-friendly spark-generated CoxOy nanoparticle-modified graphite screen-printed sensing surfaces for the determination of H2O2 in energy drinks

Electrochemical Detection of Lead in Real Samples Using Carbon Nanostructure and Inactivated E. coli as Low-Cost Sensitive Biosensor with High Electrocatalytic Performance

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