Amperometric determination of cholesterol in serum with use of a renewable surface peroxidase electrode

Charpentier, Linda J.; Murr, Nabil El

doi:10.1016/0003-2670(95)00311-8

Cited by 81 publications

(42 citation statements)

References 21 publications

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“…Triton X-100 also plays an important role in stabilizing the activity of cholesterol oxidase [2]. However at high concentrations, it inhibits the activity of cholesterol oxidase [29]. Especially, when the cholesterol oxidase electrode is repeated used, a decrease of response current is observed [30].…”

Section: Buffer Compositionmentioning

confidence: 99%

An Electrochemical Biosensor with Cholesterol Oxidase/ Sol‐Gel Film on a Nanoplatinum/Carbon Nanotube Electrode

et al. 2005

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The carbon nanotubes decorated nanoplatinum (CNT-Pt) were prepared using a chemical reduction method and a novel base electrode was constructed by intercalating CNT-Pt on the surface of a waxed graphite electrode. The results showed that the nano-particles of platinum at a waxed graphite electrode exhibits high catalytic activity for the reduction of hydrogen peroxide. The cholesterol oxidase (ChOx), chosen as a model enzyme, was immobilized with sol-gel on the CNT-Pt base electrode to construct a biosensor. The current response of the biosensor for cholesterol was very rapid (< 20 s). The linear range for cholesterol measurement was 4.0 Â 10 À6 mol/L À 1.0 Â 10 À4 mol/L with a detection limit of 1.4 Â 10 À6 mol/L. The experiments also showed that the ChOx/sol-gel/CNT-Pt biosensor was sensitive and stable in detecting cholesterol in serum samples.

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Section: Buffer Compositionmentioning

confidence: 99%

An Electrochemical Biosensor with Cholesterol Oxidase/ Sol‐Gel Film on a Nanoplatinum/Carbon Nanotube Electrode

et al. 2005

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“…The polymer lipid membrane provided a highly stable microenvironment for the immobilization of the enzyme (cholesterol oxidase), and the polymer lipid membrane has a tendency to carry high concentration of the analyte (cholesterol) molecules, due to their similar chemistry. During the experiment, a large number of cholesterol molecules undergo the oxidation on the surface of highly stabilized polymer lipid membrane containing cholesterol oxidase molecules, as shown in the following chemical reaction [22]:…”

Section: Mechanism Of Signal Generationmentioning

confidence: 99%

Biosensors Based on Lipid Modified Graphene Microelectrodes

Nikoleli

Siontorou

Nikolelis

et al. 2017

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Graphene is one of the new materials which has shown a large impact on the electronic industry due to its versatile properties, such as high specific surface area, high electrical conductivity, chemical stability, and large spectrum of electrochemical properties. The graphene material-based electronic industry has provided flexible devices which are inexpensive, simple and low power-consuming sensor tools, therefore opening an outstanding new door in the field of portable electronic devices. All these attractive advantages of graphene give a platform for the development of a new generation of devices in both food and environmental applications. Lipid-based sensors have proven to be a good route to the construction of novel devices with improved characteristics, such as fast response times, increased sensitivity and selectivity, and the possibility of miniaturization for the construction of portable biosensors. Therefore, the incorporation of a lipid substrate on graphene electrodes has provided a route to the construction of a highly sensitive and selective class of biosensors with fast response times and portability of field applications for the rapid detection of toxicants in the environment and food products.

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“…2). Electrochemical transducers have been effectively utilized for the estimation of cholesterol in the system (Charpentier & Murr, 1995;Singh et al, 2006;Zhou et al, 2006;Arya et al, 2007). Based on number and reliability of optical methods, a variety of optical transducers have been employed for cholesterol sensing, namely monitoring: luminescence, change in color of dye, fluorescence and others (Arya et al, 2008).…”

Section: Biosensors In Cardiovascular Diseasementioning

confidence: 99%

Biosensors for health applications

Gouvêa¹

2011

Biosensors for Health, Environment and Biosecurity

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Amperometric determination of cholesterol in serum with use of a renewable surface peroxidase electrode

Cited by 81 publications

References 21 publications

An Electrochemical Biosensor with Cholesterol Oxidase/ Sol‐Gel Film on a Nanoplatinum/Carbon Nanotube Electrode

An Electrochemical Biosensor with Cholesterol Oxidase/ Sol‐Gel Film on a Nanoplatinum/Carbon Nanotube Electrode

Biosensors Based on Lipid Modified Graphene Microelectrodes

Biosensors for health applications

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