Amperometric Biosensor for Hydrogen Peroxide Based on Direct Electrocatalysis by Hemoglobin Immobilized on Gold Nanoparticles/ 1,6-Diaminohexane Modified Glassy Carbon Electrode

Tang, Mingyu; Chen, Shihong; Yuan, Ruo; Gao, Fengxian; Xie, Yi

doi:10.2116/analsci.24.487

Cited by 9 publications

(5 citation statements)

References 21 publications

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“…Hemoglobin (Hb) has been extensively studied as a redox protein for direct electron transfer to AuNP-modified electrodes. Hb has very slow electron transfer rate to bulk electrodes, however, AuNPs can greatly enhance the electron transfer between Hb and electrodes with or without redox mediator. − Yuan has used this system for amperometric sensing of H 2 O 2 using Hb immobilized on multiwall carbon nanotubes (MWCNT)/AuNPs and onto AuNPs/MWCNT/chitosan composite matrices on GCE surface. Amperometric nitrite sensors have used Hb immobilized on AuNP-modified screen printed electrode and onto one-dimensional AuNP assemblies .…”

Section: Electrical and Electrochemical Sensingmentioning

confidence: 99%

Gold Nanoparticles in Chemical and Biological Sensing

et al. 2012

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Section: Electrical and Electrochemical Sensingmentioning

confidence: 99%

Gold Nanoparticles in Chemical and Biological Sensing

et al. 2012

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“…In order to ensure sensitivity and stability of an immunosensor, the antibodies must be suitably immobilized on the transducer and maintain their biological activity after the immobilization process [27]. Procedures that satisfy these requirements include the use of self-assembled monolayers on gold [28,29] and carbon surfaces [23,30], functionalized nanoparticles [31,32], and polymeric films [33,34]. It is also possible to perform the immobilization of a biomolecule by means of groups generated directly on the glassy carbon electrode surface, instead of using additional reagents to fulfil this role in the assay.…”

Section: Introductionmentioning

confidence: 99%

Label-free impedimetric immunosensor for detection of the textile azo dye Disperse Red 1 in treated water

Rocha

Ferreira

Yamanaka

2016

Sensors and Actuators B: Chemical

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The development of methodologies to detect and quantify azo dyes in treated water is required because some of these dyes exhibit genotoxic activity, even at low concentrations. Here, the construction and characterization of an impedimetric immunosensor for determination of the textile azo dye Disperse Red 1 (DR1) by means of a label-free assay is reported. Anti-DR1 antibodies (Ab anti-DR1) were successfully coupled to glassy carbon electrode (GCE) surface by bonding using electrochemically generated activated carboxylic groups. The concentration of DR1 was measured by the increase in charge transfer resistance (R CT) when the immune reaction occurred, using Fe(CN) 6 3−/4− as a probe. Electrochemical characterizations of the sensor by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were performed, and infra-red spectra were obtained to complement the electrochemical results showing immobilization of the antibodies. Under optimal conditions, the change in R CT presented a linear relationship with DR1 concentration in the range from 8.40 to 100 nM, and the sensor provided low limit of detection (LOD = 2.52 nM) and quantification (LOQ = 8.40 nM). No significant interference from other azo dyes was observed. The performance of the immunosensor was evaluated using tap water spiked with 20.0 nM DR1 and a satisfactory recovery of 98.5% was found. The proposed immunosensor is simple, relatively inexpensive, and shows good sensitivity for this application.

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“…Many techniques have been developed to quantify nitric oxide levels in biological settings. Two common approaches are the use of small-molecule labeling dyes − and specially coated electrodes. − Dyes are usually delivered intracellularly or in the interstitia where they bind reactive species and indicate species concentration through either fluorescence or chemiluminescence. However, dyes such as the widely used 4,5-diaminofluorescein respond to all species that nitrosate the substrate, including many NO decomposition products.…”

Section: Swcnt Uptake and Colocalization In Melanoma Cellsmentioning

confidence: 99%

Spatiotemporal Intracellular Nitric Oxide Signaling Captured Using Internalized, Near-Infrared Fluorescent Carbon Nanotube Nanosensors

et al. 2014

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Fluorescent nanosensor probes have suffered from limited molecular recognition and a dearth of strategies for spatial-temporal operation in cell culture. In this work, we spatially imaged the dynamics of nitric oxide (NO) signaling, important in numerous pathologies and physiological functions, using intracellular near-infrared fluorescent single-walled carbon nanotubes. The observed spatial-temporal NO signaling gradients clarify and refine the existing paradigm of NO signaling based on averaged local concentrations. This work enables the study of transient intracellular phenomena associated with signaling and therapeutics.

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Amperometric Biosensor for Hydrogen Peroxide Based on Direct Electrocatalysis by Hemoglobin Immobilized on Gold Nanoparticles/ 1,6-Diaminohexane Modified Glassy Carbon Electrode

Cited by 9 publications

References 21 publications

Gold Nanoparticles in Chemical and Biological Sensing

Gold Nanoparticles in Chemical and Biological Sensing

Label-free impedimetric immunosensor for detection of the textile azo dye Disperse Red 1 in treated water

Spatiotemporal Intracellular Nitric Oxide Signaling Captured Using Internalized, Near-Infrared Fluorescent Carbon Nanotube Nanosensors

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