Amperometric aptasensor for carcinoembryonic antigen based on a reduced graphene oxide/gold nanoparticles modified electrode

Villalonga, Anabel; Vegas, Borja; Paniagua, Gonzalo; Eguílaz, Marcos; Mayol, Beatriz; Parrado, Concepción; Rivas, Gustavo A.; Díez, Paula; Villalonga, Reynaldo

doi:10.1016/j.jelechem.2020.114511

Cited by 20 publications

(9 citation statements)

References 35 publications

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“…The bare working electrode ( Figure 1 A) was first coated with GO through electrodeposition of the nanomaterial by cyclic voltammetry [ 17 ]. As confirmed by SEM analysis ( Figure 1 B), the electrode surface was homogenously coated with crumpled nanosheets of GO.…”

Section: Resultsmentioning

confidence: 99%

“…Before immobilization, the Apt-ADA molecules were folded to the hairpin structure [ 14 , 17 ]. Briefly, 75 µL of 67 µM Apt-ADA solution was heated at 95 °C during 5 min followed by slow cooling to room temperature.…”

Section: Methodsmentioning

confidence: 99%

“…The mixture was stirred for 1 h at room temperature and further on for 16 h at 4 • C. The solution was then dialyzed vs. 50 mM sodium phosphate buffer, pH 6.0, and concentrated by 3K Amicon Ultra-05 centrifugal filter to a final concentration of 100 µM Apt-ADA. Before using, the Apt-ADA molecule was folded to the hairpin structure as described [14,17].…”

Section: Synthesis Of Chemically Modified Biomoleculesmentioning

confidence: 99%

“…2.3. Preparation of Aptamer-Functionalized Electrodes (Apt-ADA/AuNF/GO/SPE) SPE were first coated with electrodeposited GO by dropping 50 µL of 1.0 mg/mL GO dispersion in 0.1 M sodium carbonate buffer, pH 9.0 on the electrode surface and then, applying 5 scans of cyclic voltammetry between −1.5 V and 0.6 V at 25 mV/s [17]. The electrode was then washed with MilliQ water, dried under N 2 , and coated with 60 µL of a freshly prepared aqueous solution of 0.1 M HAuCl 4 in 1.0 M NH 4 Cl.…”

Section: Synthesis Of Chemically Modified Biomoleculesmentioning

confidence: 99%

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Supramolecular Enzymatic Labeling for Aptamer Switch-Based Electrochemical Biosensor

et al. 2022

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Here we report a novel labeling strategy for electrochemical aptasensors based on enzymatic marking via supramolecular host–guest interactions. This approach relies on the use of an adamantane-modified target-responsive hairpin DNA aptamer as an affinity bioreceptor, and a neoglycoconjugate of β-cyclodextin (CD) covalently attached to a redox enzyme as a labeling element. As a proof of concept, an amperometric aptasensor for a carcinoembryonic antigen was assembled on screen-printed carbon electrodes modified with electrodeposited fern-like gold nanoparticles/graphene oxide and, by using a horseradish peroxidase-CD neoglycoenzyme as a biocatalytic redox label. This aptasensor was able to detect the biomarker in the concentration range from 10 pg/mL to 1 ng/mL with a high selectivity and a low detection limit of 3.1 pg/mL in human serum samples.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Synthesis Of Chemically Modified Biomoleculesmentioning

confidence: 99%

Section: Synthesis Of Chemically Modified Biomoleculesmentioning

confidence: 99%

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Supramolecular Enzymatic Labeling for Aptamer Switch-Based Electrochemical Biosensor

et al. 2022

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“…Highly sensitive and reliable determination of biological analytes plays a significant role in the early diagnosis, real-time monitoring, and treatment of disease. − Organic field-effect transistor (OFET)-based biosensors − that emerged in recent years have attracted more and more attention from researchers. The OFET-based biosensors have many advantages such as easy large-area preparation, miniaturization, integration, small size, low cost, and fast response speed.…”

Section: Introductionmentioning

confidence: 99%

Organic Field-Effect Transistor-Based Biosensors with Enhanced Sensitivity and Reliability under Illumination for Carcinoembryonic Antigen Bioassay

et al. 2021

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Achieving biosensors of high sensitivity and reliability is extremely significant for early diagnosis and treatment of tumor diseases. Herein, a novel organic field-effect transistor (OFET)-based biosensor was developed and applied for carcinoembryonic antigen (CEA) bioassay. This OFET-based biosensor can respond sensitively to the antigen−antibody immune-recognition reaction under illumination and darkness, respectively, thereby generating electrical signal changes of source-drain current (I DS ) and threshold voltage (V th ). The OFET-based biosensor exhibits detection limits for CEA detection of 0.5 and 0.2 pM, respectively, using I DS and V th as the response signals under darkness. When a specific intensity of light is applied, light will influence the charge-carrier transport process in the conductive channel, thus causing biosignals to turn into higher electrical signal changes of photocurrent and threshold voltage under illumination. Compared with the detection results in the dark, the biosensor exhibits higher sensitivity for CEA detection under illumination with detection limits of 13.5 and 16.9 fM. Also, multisignal outputs effectively improve the reliability of the biosensor for CEA detection. Consequently, with powerful detection functions, this OFET-based biosensor is expected to become a high-performance biosensing platform for the detection of various biological substances in the future.

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Electrochemical Biosensors in Agricultural and Veterinary Applications

Bazzana

Assis

Martins

et al. 2023

Biomaterials-Based Sensors

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Amperometric aptasensor for carcinoembryonic antigen based on a reduced graphene oxide/gold nanoparticles modified electrode

Cited by 20 publications

References 35 publications

Supramolecular Enzymatic Labeling for Aptamer Switch-Based Electrochemical Biosensor

Supramolecular Enzymatic Labeling for Aptamer Switch-Based Electrochemical Biosensor

Organic Field-Effect Transistor-Based Biosensors with Enhanced Sensitivity and Reliability under Illumination for Carcinoembryonic Antigen Bioassay

Electrochemical Biosensors in Agricultural and Veterinary Applications

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