Ampelopsin Prevents Apoptosis Induced by H₂O₂ in MT-4 Lymphocytes

Abstract

Human immunodeficiency virus (HIV) infection can result in oxidative stress through production of reactive oxygen species (ROS). Simultaneously, oxidative stress is able to activate the replication of virus and lead to the apoptosis of T lymphocytes which is the defense of the immune function. Ampelopsin, belonging to the flavonoids, is a purified component from the root of a Chinese medicinal herb. Our previous studies revealed that ampelopsin could protect sensitive cells against HIV-1 infection and reduce HIV-1 antigen P24 expression. In this study, we determined whether ampelopsin, as an antioxidant, has protective effects on oxidant stress-induced apoptosis in MT-4 cells, a CD4 T lymphocyte cell line. The results indicate that ampelopsin scavenged hydroxyl radicals (.OH) and superoxide radicals (O(2).-) in a concentration-dependent manner. It significantly increased MT-4 cells viability after treatment with H(2)O(2) and inhibited H(2)O(2)-induced DNA laddering. The data from flow cytometry analysis showed that ampelopsin remarkably decreased the percentage of apoptotic cells induced by H(2)O(2). In addition, activation of caspase-3 was detected during the course of apoptosis induction. Western blot analysis showed that ampelopsin inhibited the cleavage of caspase-3 induced by H(2)O(2). All these findings might shed new light on the understanding of the anti-AIDS functions of ampelopsin by protecting T cells of persons infected with HIV.

Dihydromyricetin ameliorates the oxidative stress response induced by methylglyoxal via the AMPK/GLUT4 signaling pathway in PC12 cells

“…DMY displays many pharmacological activities, such as anti-inflammatory, cough relieving, antimicrobial, anti-hypertensive, antioxidative, hepatoprotective, and anti-carcinogenic effects (Daoud et al, 2014;Guo et al, 2014;Radhakrishnakartha et al, 2014;Zhu et al, 2014). Recently published data support the notion that DMY, as an antioxidant, protects PC12 and MT-4 cells against oxidative injury (Ye et al, 2008;Zou et al, 2014). The existing literature confirmed that DMY upregulates the level of phosphorylated AMPK (p-AMPK) in a doseand time-dependent manner (Xia et al, 2014).…”

Dihydromyricetin Reduced Bcl-2 Expression via p53 in Human Hepatoma HepG2 Cells

“…It was reported that the DHM can reach more than 30% in the tender stem and leaves of vine tea [1]. DHM has numerous pharmacological activities, such as anti-inflammatory, relieving cough, antimicrobial activity, anti-hypertension, anti-oxidation, hepatoprotective effect and anti-carcinogenic effect [2]–[5]. Recently, plenty of data supported that DHM could inhibit the growth and metastasis of prostate cancer in vitro and in vivo [6].…”

Antioxidant Flavonoids from the Twigs of Stewartia koreana

“…Among six isolated compounds, compound 1 exhibited higher activity with IC 50 value of 2.6 μg/ml than vitamin C, used as a positive control (IC50 value, 6.4 μg/ml) (Table IV). It was reported that compound 1, ampelopsin has protective effect on oxidant stress-induced apoptosis induced by H2O2 in MT-4 cells, antitumor effects, inhibitory effects on angiogenesis, and hepatoprotective activity (Murakami et al, 2004;Zeng et al, 2004;Luo et al, 2006;Ye et al, 2008). The results from free radical scavenging systems revealed that the ethyl acetate soluble fraction of S. koreana, and isolated compounds 1-3 may be useful for the treatment of various oxidative damage.…”