AMPA-induced dark cell degeneration is associated with activation of caspases in pyramidal neurons of the rat hippocampus

Coles, Jennifer; Oomman, Sowmini; Henne, W.; Bliss, Richard; Hoffman, Trenton; Strahlendorf, Howard K.; Strahlendorf, Jean C.

doi:10.1016/j.neulet.2008.02.075

Cited by 5 publications

(3 citation statements)

References 24 publications

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“…This enzyme, in turn, start the formation of dark neurons and their next degeneration. When inhibiting caspases with the pancaspase inhibitor FK011, a decrease in the changes characteristic of dark neurons has been achieved [19].…”

Section: Discussionmentioning

confidence: 99%

“…In further studies, it is necessary to study at what postoperative period the p53 protein expression in neurons decreases and the number of dark neurons in the hippocampal formation returns to normal values. Most likely, this should occur after the 14th day after surgery, since it is known that migrating neurons from the dentate gyrus to the hippocampus begin to synthesize neurotransmitters and fully perform their function starting from the 14th day of their appearance in the pyramidal layer of the hippocampus [19,20,32].…”

Section: Discussionmentioning

confidence: 99%

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Expression of the P53 Protein and Morphological Changes in Neurons in the Pyramidal Layer of the Hippocampus After Simulation of Surgical Interventions in the Nasal Cavity in Rats

Кастыро

Mikhalskaia

Khamidulin

et al. 2023

Cell Physiol Biochem

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Background/Aims: to determine the role of surgical stress on the formation of p53-positive and dark neurons (DN) in the hippocampus, and to examine the parallelism of their formation in the pyramidal layer of the hippocampus. Methods: Simulated septoplasty was performed on 20 Wistar rats. The hippocampus and dentate gyrus (DG) were examined, in which the number of DN and p53-positive neurons was determined at 2, 4 and 6 days after surgery. In each rat, 10 brain slices were stained with antibodies to p53 protein with Meyer’s haematoxylin and 10 slices were stained with Nissl toluidine blue. Hippocampal subfields CA1, CA2, CA3 and DG were studied. In the pyramidal subfield layer, the absolute number of neurons that were nuclear antibody-positive to p53 protein was counted, as well as the number of dark neurons. The counting area in each subfield was 20934±1260 µm2. Neurons are counted using the Aperio ImageScope program. For the histological specimen analysis, the ImageJ software was used. The data obtained using cell counting methods were presented as mean ± SE. Then, they were compared between both groups using a t-test SPSS 21software. Results: Compared with the control group (n = 5), the number of DN and p53-positive neurons increased in experimental animals at all periods. A direct relationship was obtained between the increase in the number of DN and p53-positive neurons in the hippocampal formation. Septoplasty simulation in rats results in the pathogenetic cascades onset, which, in its turn, changes the morpho-functional properties of neurons of the pyramidal layer of the hippocampus and contributes to their neuroplasticity. Activation of NMDS receptors of neurons during stress apparently, initiates two ways of neuron life – the beginning of p53 protein expression and the DN formation. Both ways can finally lead to apoptosis. Conclusion: The formation of dark neurons and the expression of the p53 protein in them are most likely to be interconnected and can probably provide neuroprotective mechanisms.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Expression of the P53 Protein and Morphological Changes in Neurons in the Pyramidal Layer of the Hippocampus After Simulation of Surgical Interventions in the Nasal Cavity in Rats

Кастыро

Mikhalskaia

Khamidulin

et al. 2023

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…This enzyme, in its turn, starts the formation of dark neurons and their next degeneration. When inhibiting caspases with the pancaspase inhibitor FK011, a decrease in the changes characteristic of dark neurons has been achieved [19].…”

Section: Discussionmentioning

confidence: 99%

Role of Septoplasty as a Stressor in P53 and Dark Neurons Formation During Septoplasty Simulation in Rats

Кастыро¹,

Mikhalskaia²,

Khamidulin³

et al. 2022

ArveMED

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The aim of this study was to determine the role of surgical stress on the formation of p53-positive and dark neurons (DN) in the hippocampus, and to examine the parallelism of their formation in the pyramidal layer of the hippocampus. Simulated septoplasty was performed on 20 Wistar rats. The hippocampus and dentate gyrus (DG) were examined, in which the number of DN and p53-positive neurons was determined at 2, 4 and 6 days after surgery. Compared with the control group (n = 5), the number of DN and p53-positive neurons increased in experimental animals at all periods. A direct relationship was obtained between the increase in the number of DN and p53-positive neurons in the hippocampal formation. Septoplasty simulation in rats results in the pathogenetic cascades onset, which, in its turn, changes the morpho-functional properties of neurons of the pyramidal layer of the hippocampus and contributes to their neuroplasticity. Activation of NMDS receptors of neurons during stress, apparently, initiates two ways of neuron life – the beginning of p53 protein expression and the DN formation. Both ways can finally lead to apoptosis. The formation of dark neurons and the expression of the p53 protein in them are most likely to be interconnected and can probably provide neuroprotective mechanisms.

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Neuroprotective effects of leptin following kainic acid-induced status epilepticus

Obeid¹,

Frank²,

Médina³

et al. 2010

Epilepsy & Behavior

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AMPA-induced dark cell degeneration is associated with activation of caspases in pyramidal neurons of the rat hippocampus

Cited by 5 publications

References 24 publications

Expression of the P53 Protein and Morphological Changes in Neurons in the Pyramidal Layer of the Hippocampus After Simulation of Surgical Interventions in the Nasal Cavity in Rats

Expression of the P53 Protein and Morphological Changes in Neurons in the Pyramidal Layer of the Hippocampus After Simulation of Surgical Interventions in the Nasal Cavity in Rats

Role of Septoplasty as a Stressor in P53 and Dark Neurons Formation During Septoplasty Simulation in Rats

Neuroprotective effects of leptin following kainic acid-induced status epilepticus

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