&amp;lt;italic&amp;gt;In vitro&amp;lt;/italic&amp;gt; identification of DNA-binding motif for the new zinc finger protein AtYY1

Wu, Xueping; Cheng, Yong; Tian, Li; Wang, Zhao; Liu, Jinyuan

doi:10.1093/abbs/gms020

Cited by 6 publications

(9 citation statements)

References 27 publications

(24 reference statements)

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“…Besides their role in sister chromatid cohesion, cohesins are very important for various meiosis‐specific events including chromosomal axis formation, synaptonemal complex formation and reciprocal recombination (Ding et al., 2016; Ishiguro, Kim, Fujiyama‐Nakamura, Shigeaki Kato, & Watanabe, 2011). Ying Yang 1 is an architectural protein with a critical role in connecting higher‐order chromatin folding in animals (Beagan et al., 2017) and in Arabidopsis (Wu et al., 2012). HMG proteins have been suggested to be involved in initial interactions between homologues, prior to proper pairing, through their potential interaction with AT‐rich sites (Subirana & Messeguer, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, the impact of these axis structures on initial chromosome interactions for specific recognition and pairing remains to be elucidated. Other architectural proteins like CTCF and Ying Yang 1, which have a critical function connecting higher-order chromatin folding in animals, could also contribute to facilitate initial homologous chromosome interactions (Beagan et al, 2017;Wu, Cheng, Li, Wang, & Liu, 2012) and might play a putative role in meiosis. In fact, CTCF colocalizes with cohesin subunits at many chromosomal positions and form DNA loops (Hirano, 2006;Nasmyth & Haering, 2005;Rubio et al, 2008;Wendt et al, 2008).…”

Section: Core Ideasmentioning

confidence: 99%

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Sequence analysis of wheat subtelomeres reveals a high polymorphism among homoeologous chromosomes

Aguilar

Prieto

2020

The Plant Genome

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Bread wheat, Triticum aestivum L., is one of the most important crops in the world. Understanding its genome organization (allohexaploid; AABBDD; 2n = 6x = 42) is essential for geneticists and plant breeders. Particularly, the knowledge of how homologous chromosomes (equivalent chromosomes from the same genome) specifically recognize each other to pair at the beginning of meiosis, the cellular process to generate gametes in sexually reproducing organisms, is fundamental for plant breeding and has a big influence on the fertility of wheat plants. Initial homologous chromosome interactions contribute to specific recognition and pairing between homologues at the onset of meiosis. Understanding the molecular basis of these critical processes can help to develop genetic tools in a breeding context to promote interspecific chromosome associations in hybrids or interspecific genetic crosses to facilitate the transfer of desirable agronomic traits from related species into a crop like wheat. The terminal regions of chromosomes, which include telomeres and subtelomeres, participate in chromosome recognition and pairing. We present a detailed molecular analysis of subtelomeres of wheat chromosome arms 1AS, 4AS, 7AS, 7BS and 7DS. Results showed a high polymorphism in the subtelomeric region among homoeologues (equivalent chromosomes from related genomes) for all the features analyzed, including genes, transposable elements, repeats, GC content, predicted CpG islands, recombination hotspots and targeted sequence motifs for relevant DNA-binding proteins. These polymorphisms might be the molecular basis for the specificity of homologous recognition and pairing in initial chromosome interactions at the beginning of meiosis in wheat. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Section: Discussionmentioning

confidence: 99%

Section: Core Ideasmentioning

confidence: 99%

Sequence analysis of wheat subtelomeres reveals a high polymorphism among homoeologous chromosomes

Aguilar

Prieto

2020

The Plant Genome

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“…TRM1 can specifically bind a YY1-like DNA site located within the rbcS-m3 3 0 untranslated region (Xu et al, 2001). Through an in vitro DNA-binding sequence discovery approach, Arabidopsis YY1 (AtYY1) was shown to bind an 11 bp G-rich DNA motif (Wu et al, 2012). More recently, Lai et al (2014) reported that AtYY1 can interact with MED18 to suppress the expression of some disease susceptibility genes and enhance resistance to fungal infection.…”

Section: Introductionmentioning

confidence: 99%

A Dual-Function Transcription Factor, AtYY1, Is a Novel Negative Regulator of the Arabidopsis ABA Response Network

et al. 2016

Molecular Plant

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Abscisic acid (ABA) plays crucial roles in plant growth and development, as well as in response to various environmental stresses. To date, many regulatory genes involved in the ABA response network have been identified; however, their roles have remained to be fully elucidated. In this study, we identified AtYY1, an Arabidopsis homolog of the mammalian C2H2 zinc-finger transcription factor Yin Yang 1 (YY1), as a novel negative regulator of the ABA response. AtYY1 is a dual-function transcription factor with both repression and activation domains. The expression of AtYY1 was induced by ABA and stress conditions including high salt and dehydration. The yy1 mutant was more sensitive to ABA and NaCl than the wild-type, while overexpressing AtYY1 plants were less sensitive. AtYY1 loss also enhanced ABA-induced stomatal closing and drought resistance. Moreover, AtYY1 can bind the ABA REPRESSOR1 (ABR1) promoter and directly upregulate ABR1 expression, as well as negatively regulate ABA- and salt-responsive gene expression. Additional analysis indicated that ABA INSENSITIVE4 (ABI4) might positively regulate AtYY1 expression and that ABR1 can antagonize this regulation. Our findings provide direct evidence that AtYY1 is a novel negative regulator of the ABA response network and that the ABI4-AtYY1-ABR1 regulatory pathway may fine-tune ABA-responsive gene expression in Arabidopsis.

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“…The zinc finger transcription factor Yin Yang 1 (YY1, At4g06634) becomes hyper-acetylated upon KDAC inhibition ( Hartl et al, 2017 ). YY1 plays important roles in the abscisic acid (ABA) response and was recently identified as regulated by phosphorylation ( Wu et al, 2012 ; Wu and Li, 2017 ). Hence, it will be interesting to investigate the interplay between phosphorylation and acetylation on YY1.…”

Section: Transcription Factors and Chromatin Remodeling Complexesmentioning

confidence: 99%

Beyond Histones: New Substrate Proteins of Lysine Deacetylases in Arabidopsis Nuclei

et al. 2018

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The reversible acetylation of lysine residues is catalyzed by the antagonistic action of lysine acetyltransferases and deacetylases, which can be considered as master regulators of their substrate proteins. Lysine deacetylases, historically referred to as histone deacetylases, have profound functions in regulating stress defenses and development in plants. Lysine acetylation of the N-terminal histone tails promotes gene transcription and decondensation of chromatin, rendering the DNA more accessible to the transcription machinery. In plants, the classical lysine deacetylases from the RPD3/HDA1-family have thus far mainly been studied in the context of their deacetylating activities on histones, and their versatility in molecular activities is still largely unexplored. Here we discuss the potential impact of lysine acetylation on the recently identified nuclear substrate proteins of lysine deacetylases from the Arabidopsis RPD3/HDA1-family. Among the deacetylase substrate proteins, many interesting candidates involved in nuclear protein import, transcriptional regulation, and chromatin remodeling have been identified. These candidate proteins represent key starting points for unraveling new molecular functions of the Arabidopsis lysine deacetylases. Site-directed engineering of lysine acetylation sites on these target proteins might even represent a new approach for optimizing plant growth under climate change conditions.

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<italic>In vitro</italic> identification of DNA-binding motif for the new zinc finger protein AtYY1

Cited by 6 publications

References 27 publications

Sequence analysis of wheat subtelomeres reveals a high polymorphism among homoeologous chromosomes

Sequence analysis of wheat subtelomeres reveals a high polymorphism among homoeologous chromosomes

A Dual-Function Transcription Factor, AtYY1, Is a Novel Negative Regulator of the Arabidopsis ABA Response Network

Beyond Histones: New Substrate Proteins of Lysine Deacetylases in Arabidopsis Nuclei

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