&amp;lt;italic&amp;gt;Arabidopsis&amp;lt;/italic&amp;gt; inositol 1,3,4-trisphosphate 5/6 kinase 2 is required for seed coat development

Tang, Yong; Tan, Shutang; Hou, Xue

doi:10.1093/abbs/gmt039

Cited by 16 publications

(9 citation statements)

References 32 publications

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“…However, the isolated null-mutant itpk3-1 had identical total phosphorus and phytate concentrations as the wild-type, Col-0, even under Zn-deficiency ( Figure 6 ). This is in agreement with a previous study ( Tang et al, 2013 ). Furthermore, Zn, Fe, Mn, and the RZn were also maintained in the itpk3-1 seeds, despite a minor tendency for reduced iron levels, excluding this gene as a major determinant of seed micronutrients.…”

Section: Discussionsupporting

confidence: 94%

“…RT-PCR confirmed that the ITPK3 expression was lost in the itpk3-1 mutant seeds, both in +Zn and -Zn ( Figure 6B ). The ITPK gene family includes four members ( Tang et al, 2013 ), of which only ITPK1 is also significantly expressed in seeds. Intriguingly, the ITPK1 transcript level was also reduced in the itpk3-1 mutant, especially in -Zn ( Figure 6B ).…”

Section: Resultsmentioning

confidence: 99%

“…The primers used were AT5G16760 ( ITPK1 , 5′-TAGGGATGCCAAAGATGCTAATA-3′ and 5′-GTCCCAGAAGAACTCAGTCAACA-3′); AT4G33770 ( ITPK2 , 5′-CAAGGTATTTGTGGTGGGTGAT-3′ and 5′-GAGGGTCCAAGTCTGCGTTAT-3′); AT4G08170 ( ITPK3 , 5′-ATCGTCGCCGTGTTCGTTAGT-3′ and 5′-AAACGGACCCTGCTCTGAAAGT-3′); AT2G43980 ( ITPK4 , 5′-GCCATCTCGGGTAGAGGACTTT-3′ and 5′-AGCAGTTCAGTTCAATGGACAAGA-3′). The primers of ITPK1 , ITPK2 , and ITPK4 were used previously ( Tang et al, 2013 ). For the PCR procedure, a 15 μl reaction was carried out, containing 6 μl 20X diluted cDNA, 7.5 μl SYBR Green Supermix (KAPA Biosystems, USA), 0.3 μl forward primers, 0.3 μl reverse primers, and 0.9 μl RNase-free H 2 O.…”

Section: Methodsmentioning

confidence: 99%

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Natural Genetic Variation of Seed Micronutrients of Arabidopsis thaliana Grown in Zinc-Deficient and Zinc-Amended Soil

2016

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The quality of edible seeds for human and animal nutrition is crucially dependent on high zinc (Zn) and iron (Fe) seed concentrations. The micronutrient bioavailability is strongly reduced by seed phytate that forms complexes with seed cations. Superior genotypes with increased seed Zn concentrations had been identified, but low micronutrient seed levels often prevail when the plants are grown in Zn-deficient soils, which are globally widespread and correlate with human Zn-deficiency. Here, seed Zn concentrations of Arabidopsis accessions grown in Zn-deficient and Zn-amended conditions were measured together with seed Fe and manganese (Mn), in a panel of 108 accessions. By applying genome-wide association, de novo candidate genes potentially involved in the seed micronutrient accumulation were identified. However, a candidate inositol 1,3,4-trisphosphate 5/6-kinase 3 gene (ITPK3), located close to a significant nucleotide polymorphism associated with relative Zn seed concentrations, was dispensable for seed micronutrients accumulation in Col-0. Loss of this gene in itpk3-1 did neither affect phytate seed levels, nor seed Zn, Fe, and Mn. It is concluded that large natural variance of micronutrient seed levels is identified in the population and several accessions maintain high seed Zn despite growth in Zn-deficient conditions.

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Section: Discussionsupporting

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Natural Genetic Variation of Seed Micronutrients of Arabidopsis thaliana Grown in Zinc-Deficient and Zinc-Amended Soil

2016

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“…A rice MAPK, BWMK1 encoded by an HT salt-responsive DEG, could phosphorylate the OsEREBP1 transcription factor for binding to the GCC box element (AGCCGCC), which is a basic component of several pathogenesis-related gene promoters [ 39 ]. Inositol 1,3,4-trisphosphate 5/6-kinase (IPTK) encoded by two HT salt-responsive DEGs, phosphorylates inositol 1,3,4-trisphosphate to form inositol 1,3,4,5-tetrakisphosphate and inositol 1,3,4,6, tetrakisphosphate, which are ultimately converted to inositol hexaphosphate (IP6) and play roles in plant growth and development [ 40 ]. In rice, the T-DNA mutant of an IPTK gene showed reduced osmolyte accumulation and growth under drought conditions, and some genes involved in osmotic adjustment and reactive oxygen species scavenging were down-regulated.…”

Section: Discussionmentioning

confidence: 99%

Downstream components of the calmodulin signaling pathway in the rice salt stress response revealed by transcriptome profiling and target identification

et al. 2018

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BackgroundCalmodulin (CaM) is an important calcium sensor protein that transduces Ca2+ signals in plant stress signaling pathways. A previous study has revealed that transgenic rice over-expressing the calmodulin gene OsCam1–1 (LOC_Os03g20370) is more tolerant to salt stress than wild type. To elucidate the role of OsCam1–1 in the salt stress response mechanism, downstream components of the OsCam1–1-mediated response were identified and investigated by transcriptome profiling and target identification.ResultsTranscriptome profiling of transgenic ‘Khao Dawk Mali 105’ rice over-expressing OsCam1–1 and wild type rice showed that overexpression of OsCam1–1 widely affected the expression of genes involved in several cellular processes under salt stress, including signaling, hormone-mediated regulation, transcription, lipid metabolism, carbohydrate metabolism, secondary metabolism, photosynthesis, glycolysis, tricarboxylic acid (TCA) cycle and glyoxylate cycle. Under salt stress, the photosynthesis rate in the transgenic rice was slightly lower than in wild type, while sucrose and starch contents were higher, suggesting that energy and carbon metabolism were affected by OsCam1–1 overexpression. Additionally, four known and six novel CaM-interacting proteins were identified by cDNA expression library screening with the recombinant OsCaM1. GO terms enriched in their associated proteins that matched those of the differentially expressed genes affected by OsCam1–1 overexpression revealed various downstream cellular processes that could potentially be regulated by OsCaM1 through their actions.ConclusionsThe diverse cellular processes affected by OsCam1–1 overexpression and possessed by the identified CaM1-interacting proteins corroborate the notion that CaM signal transduction pathways compose a complex network of downstream components involved in several cellular processes. These findings suggest that under salt stress, CaM activity elevates metabolic enzymes involved in central energy pathways, which promote or at least maintain the production of energy under the limitation of photosynthesis.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1538-4) contains supplementary material, which is available to authorized users.

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“…One of the interesting observation was the earliest expression of TaITPK3 and TaITPK4 (at 30 min) followed by TaIPK1 (at 45 min) suggesting the sequential expression cascades for the PA pathway genes. In fact, one of the intermediate pathway genes in Arabidopsis , AtITPK2 was also highly induced upon ABA treatment in seeds [ 52 ]. ABA is also interlinked with the drought tolerance, for example, OsITPK genes also showed differential response against drought stress and ABA signaling [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

Hormonal Regulation and Expression Profiles of Wheat Genes Involved during Phytic Acid Biosynthesis Pathway

Aggarwal

Shukla

Bhati

et al. 2015

Plants

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Phytic acid (PA) biosynthesis pathway genes were reported from multiple crop species. PA accumulation was enhanced during grain filling and at that time, hormones like Abscisic acid (ABA) and Gibberellic acid (GA3) interplay to control the process of seed development. Regulation of wheat PA pathway genes has not yet been reported in seeds. In an attempt to find the clues for the regulation by hormones, the promoter region of wheat PA pathway genes was analyzed for the presence of cis-elements. Multiple cis-elements of those known to be involved for ABA, GA3, salicylic acid (SA), and cAMP sensing were identified in the promoters of PA pathway genes. Eight genes (TaIMP, TaITPK1-4, TaPLC1, TaIPK2 and TaIPK1) involved in the wheat PA biosynthesis pathway were selected for the expression studies. The temporal expression response was studied in seeds treated with ABA and GA3 using quantitative real time PCR. Our results suggested that exogenous application of ABA induces few PA pathway genes in wheat grains. Comparison of expression profiles for PA pathway for GA3 and ABA suggested the antagonistic regulation of certain genes. Additionally, to reveal stress responses of wheat PA pathway genes, expression was also studied in the presence of SA and cAMP. Results suggested SA specific differential expression of few genes, whereas, overall repression of genes was observed in cAMP treated samples. This study is an effort to understand the regulation of PA biosynthesis genes in wheat.

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<italic>Arabidopsis</italic> inositol 1,3,4-trisphosphate 5/6 kinase 2 is required for seed coat development

Cited by 16 publications

References 32 publications

Natural Genetic Variation of Seed Micronutrients of Arabidopsis thaliana Grown in Zinc-Deficient and Zinc-Amended Soil

Natural Genetic Variation of Seed Micronutrients of Arabidopsis thaliana Grown in Zinc-Deficient and Zinc-Amended Soil

Downstream components of the calmodulin signaling pathway in the rice salt stress response revealed by transcriptome profiling and target identification

Hormonal Regulation and Expression Profiles of Wheat Genes Involved during Phytic Acid Biosynthesis Pathway

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