<i>SIRT1</i> gene polymorphisms associated with carcass traits in Luxi cattle

Abstract

Abstract. SIRT1 is the gene that codes for Sirtuin 1, an NAD (nicotinamide adenine dinucleotide)-dependent class III histone deacetylase. This gene plays a key role in adipose tissue and muscle development in animals. Chinese Luxi cattle (n = 169) were selected to identify SIRT1 SNPs (single nucleotide polymorphisms) and investigate the relationship of these SNPs with carcass traits. Five SNPs (g.-382G > A, g.-274C > G, g.17324T > C, g.17379A > G, and g.17491G > A) were identified by direct sequencing. SNPs g.-382G > A and g.-274C > G were located within the promoter region of this gene. SNP g.-382G > A was significantly associated with dressing percentage, meat percentage, and striploin and ribeye weights, and the g.-274C > G polymorphism had a strong effect on carcass, tenderloin, and high rib weights in Luxi cattle. These findings will provide possible clues for the biological roles of SIRT1 underlying beef cattle carcass traits.

Determination of a possible relationship between a single nucleotide polymorphism (SNP) in the promoter region of the <i>SIRT1</i> gene and production and reproduction traits in the Agerolese cattle breed

“…Milk quality was significantly affected only in terms of FC (3.84 % vs. 3.55 % for CC and GG cows, respectively; P < 0.05), with no difference in protein content. When production data during standard lactation are considered, no differences were found concern- Liu et al (2017) ing the quality of milk. Moreover, CG animals yielded a greater amount of milk when compared to cows belonging to CC genotype (5018.30 kg vs. 4165.67 kg; P < 0.05).…”

“…To date, only few studies have been conducted in order to find a possible relationship between SIRT1 gene polymorphisms and livestock performance traits. Nevertheless, the SIRT1 gene seems to be a potentially useful genetic marker for carcass and body measurement characteristics (Li et al, 2013a, b;Gui et al, 2014;Liu et al, 2017). In particular, Li et al (2013a, b) found a positive association between the c.-274C > G polymorphism and growth traits in Nanyang cattle suggesting that the presence of the G allelic variant could be responsible for a decreased gene promoter activity that, in turn, is responsible for the improved growth traits observed in CG and GG animals.…”

“…The mammalian sirtuin family consists of seven members (SIRT1-7). Each sirtuin is characterized by a conserved 275 amino acid catalytic core domain and peculiar N-terminal and/or C-terminal sequences of variable length (Michan and Sinclair, 2007). Mammalian sirtuins also differ in their subcellular localization.…”

“…SIRT1, SIRT6 and SIRT7 are predominately in the nucleus, but SIRT1 also shows important cytoplasmic functions. SIRT2 resides most prominently in the cytoplasm, while SIRT3-5 are mitochondrial sirtuins (Michan and Sinclair, 2007;Michishita et al, 2005;North et al, 2003;Vaziri et al, 2001).…”

“…Later, Gui et al (2014Gui et al ( , 2015 reported many noncoding mutations in the 3 UTR of the SIRT1 gene (g.25764G > A, g.25846A > G, g.25868T > C, g.25751A > C). Some of the abovementioned SNPs were found to be associated with various growth and carcass traits in Nanyang, Qinchuan and Luxi cattle breeds (Li et al, 2013a, b;Gui et al, 2014Gui et al, , 2015Liu et al, 2017).…”