<i>PSAP</i> gene variants and haplotypes reveal significant effects on carcass and meat quality traits in Chinese Simmental-cross cattle

Abstract

Abstract. Prosaposin (PSAP) is the precursor protein of four small lysosomal glycoproteins and plays vital roles in muscle growth and development of beef cattle. The aim of this study is to evaluate the association between PSAP gene polymorphisms with carcass composition and meat quality traits of Chinese Simmental-cross cattle. In the present study, six SNPs were identified within intron 10 and exon 11 of the PSAP gene from 380 beef cattle by direct DNA sequencing, and 47 traits of carcass composition and meat quality were measured in the studied population. Then, the relationship between variants of PSAP gene with economic traits was analyzed using statistical methods. The result revealed that I10-65G > A, I10-313C > T, E11-87C > T, and E11-93C > G were significantly associated with carcass traits, such as dressing percentage, fat coverage rate, carcass depth, and hind leg width, and I10-162C > T and I10-274C > T were significantly associated with marbling score traits. In addition, the individuals with GCCCGC haplotype showed significant higher dressed weight, dressing percentage and rib eye area (p < 0.01), and the individuals with ACCTCC haplotype showed a significant association with the pH of beef and backfat thickness in the cattle population (p < 0.05). Our results indicate that these SNPs and haplotypes of the PSAP gene are possible important genetic factors that influence carcass composition and meat quality traits, and they may be useful markers in future marker-assisted selection (MAS) programs in beef cattle breeding and production.

Individual and combined effects of &lt;i&gt;CAPN1&lt;/i&gt;, &lt;i&gt;CAST&lt;/i&gt;, &lt;i&gt;LEP&lt;/i&gt; and &lt;i&gt;GHR&lt;/i&gt; gene polymorphisms on carcass characteristics and meat quality in Holstein bulls

“…Recently, the trend of improving these programmes has gradually changed from traditional phenotypical selection methods to genotypic selection by utilizing molecular markers and a better understanding of DNA polymorphisms that have an effect on carcass and meat quality may lead to important applications through marker-assisted selection (MAS) programmes (Guo et al, 2016). However, genetic variations regarding both the quantity and quality of beef already exist among breeds and even among different populations of the same breed (Burrow et al, 2001).…”

Expression analysis and single-nucleotide polymorphisms of MLPH and PMEL17 genes associated with melanin deposition in Xuefeng black-bone chickens

“…Missense mutation in exon could lead to amino acid sequences changes, which affect the structure and function of proteins, thereby causing phenotype differences between individuals (Cai et al 2019;Bai et al 2021). Meanwhile, the intron is involved in the splicing process of genes, which changes the coding of the amino acid through influencing the splicing efficiency or accuracy of gene translation, thereby altering the phenotype of individuals (Mayo 2008; Guo et al 2016). This indicated that phenotype may be affected by multiple mutation locus Santos et al 2017;Dudásová et al 2021).…”

Functional Analysis of Haplotypes in Bovine PSAP Gene and Their Relationship with Beef Cattle Production Traits

“…PSAP has an important function in the Caspase, MAPK, PI3K/Akt, and TGF-β pathways, which have potential function in animal breeding [ 17 ]. For example, Guo found six SNPs in the 10th intron and 11th exon of PSAP that influenced carcass and meat quality traits [ 18 ]. In this study, 13 variations belonging to the 14th intron, 15th exon, 3’ UTR, and downstream region of PSAP were found in four cattle breeds.…”

“…At the same time, PSAP regulates caspase, mitogen-activated protein kinase (MAPK), phosphatidylinositol 3’-kinase/AKT serine/threonine kinase 1 (PI3K/Akt), and transforming growth factor-β (TGF-β) pathways and has an important relationship with animal growth and development [ 17 ]. PSAP has been reported as a potential target gene in cattle breeding with functional single-nucleotide polymorphism (SNP) and insertion/deletion (InDels) loci associated with cattle morphological traits [ 18 , 19 ]. Considering their location, we speculate that the cattle PSAP gene also has other functional variations.…”