&amp;lt;i&amp;gt;In Vitro&amp;lt;/i&amp;gt; Micropropagation of a Valuable  Medicinal Plant, &amp;lt;i&amp;gt;Plectranthus amboinicus&amp;lt;/i&amp;gt;

Rahman, Zuraida Ab; Noor, Erny Sabrina Mohd; Ali, Mohd Shukuri Mohamad; Mirad, Razali; Othman, Ayu Nazreena

doi:10.4236/ajps.2015.68113

Cited by 19 publications

(15 citation statements)

References 6 publications

(3 reference statements)

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“…MS basal media (1962) is the most commonly used plant culture media. As presented in Table 4, it has a very high salt content, nevertheless, it is the preferred medium for the growth of several species (Alvarenga et al 2015;Grzegorczyk-Karolak et al 2015;Rahman et al 2015). It has been demonstrated that different MS salt concentrations influence growth in several species (Assis et al 2012;Martins et al 2015;Shekhawat et al 2015;Singh et al 2015).…”

Section: Improvements In Traditional Culturing Techniquesmentioning

confidence: 99%

In vitro plant tissue culture: means for production of biological active compounds

Espinosa-Leal

Puente-Garza

García‐Lara

2018

Planta

418

206

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Plant tissue culture as an important tool for the continuous production of active compounds including secondary metabolites and engineered molecules. Novel methods (gene editing, abiotic stress) can improve the technique. Humans have a long history of reliance on plants for a supply of food, shelter and, most importantly, medicine. Current-day pharmaceuticals are typically based on plant-derived metabolites, with new products being discovered constantly. Nevertheless, the consistent and uniform supply of plant pharmaceuticals has often been compromised. One alternative for the production of important plant active compounds is in vitro plant tissue culture, as it assures independence from geographical conditions by eliminating the need to rely on wild plants. Plant transformation also allows the further use of plants for the production of engineered compounds, such as vaccines and multiple pharmaceuticals. This review summarizes the important bioactive compounds currently produced by plant tissue culture and the fundamental methods and plants employed for their production.

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Section: Improvements In Traditional Culturing Techniquesmentioning

confidence: 99%

In vitro plant tissue culture: means for production of biological active compounds

Espinosa-Leal

Puente-Garza

García‐Lara

2018

Planta

418

206

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“…e lack or slow shooting in PGR-free MS media is observed by many researchers in several Aloe species [15,19,21,22,[25][26][27][28][29]. e use of combination of auxins and cytokinins with MS media is known to be effective for optimal in vitro shoot proliferation in many species including aloes as compared to the use of either group of PGRs [11,25,30,31]. But establishing the best combinations and concentrations of auxins and cytokines for better shooting of explants is often tricky.…”

Section: Shooting Responsesmentioning

confidence: 99%

In VitroMicropropagation ofAloe adigratanaReynolds Using Offshoot Cuttings

Niguse

Sbhatu

Abraha

2020

The Scientific World Journal

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is study aimed at developing a suitable and reproducible protocol for in vitro micropropagation of Aloe adigratana Reynolds using explants from offshoots with the help of the most commonly used plant growth regulators (PGRs). Explants were initiated in full-strength Murashige and Skoog (MS) media enriched with 0.2 mg/L benzylaminopurine (BAP) + 0.2 mg/L naphthaleneacetic acid (NAA). Shooting experiment was conducted in full-strength MS media enriched with 0/0, 0.5/0.5, 1.0/0.5, 1.5/0.5, and 2.0/ 0.5 mg/L BAP/NAA. Likewise, rooting experiment was carried out in half-strength MS media enriched with NAA at 0.5, 1.0, and 1.5 mg/L and indol-3-butyric acid (IBA) at 0.5, 1.0, and 1.5 mg/L. Finally, acclimatization study was conducted in greenhouse, nursery, and open field. e shooting response of the plant was best in MS media enriched with 1.0 mg/L BAP + 0.5 mg/L NAA.is media formulation resulted in the shortest mean number of days to shooting (14.00 ± 2.30 days), the highest mean shoot number (4.00 ± 3.40), and the second highest mean shoot length (8.60 ± 2.10 cm). IBA enhanced rooting at higher concentrations (1.0 and 1.5 mg/L), but NAA did the same at lower concentrations (0.5 and 1.0 mg/L). All plantlets (n � 62) survived primary acclimatization. Secondary acclimatization in composted and matured soil media under nursery and open field (sun light) condition produced 88 to 93% survival rate. e death of plantlets in the secondary acclimatization is accounted to mechanical damage. is study demonstrated that the tested PGRs were useful in enhancing the in vitro micropropagation of the plant. Future studies need to focus on optimizing the protocol for large-scale, commercial micropropagation.

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“…1i). The superiority of IBA over other auxins on in vitro rooting has been reported in Plectranthus ambonicus (Rhaman et al 2015) and Glinus lotoides (Teshome and Feyissa 2015). The number of roots per shoot was increased with increasing concentration of IBA from 0.1 to 1.0 mg/l, whereas subsequent concentrations decreased the frequency of rooting.…”

Section: Rooting Of Regenerated Shootsmentioning

confidence: 72%

Mass propagation of Plectranthus bourneae Gamble through indirect organogenesis from leaf and internode explants

Thaniarasu

Kumar

Rao

2016

Physiol Mol Biol Plants

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The present study describes the plant propagation via indirect organogenesis from in vitro derived leaf and internode explants of Plectranthus bourneae, an endemic plant to south India. Leaf and internodal explants successfully callused on Murashige and Skoog medium (MS) supplemented with different concentrations of auxins [2,4-D (2, 4-dichlorophenoxyacetic acid), NAA (α-naphthalene acetic acid), IAA (indole-3 acetic acid), IBA (indole-3-butyric acid) and PIC (Picloram); 0.1-2.0 mg/l] in combination with BA (6-benzyladenine) (0.5 mg/l). Maximum callus induction (98 %) was achieved from leaf explant followed by internodal explant (89 %) at 1.0 mg/l NAA, 0.5 mg/l BA. Leaf derived callus showed better shoot regeneration (29.71 shoots) on MS medium containing 1.0 mg/l KN (kinetin), 0.7 mg/l NAA, and 50 mg/l CH (casein hydrolysate) followed by internodal callus (19.71). A maximum of 19.14 roots/shoot was observed at 1.0 mg/l IBA. The rooted plantlets were successfully hardened and transferred to greenhouse condition with 80 % survival. This system could be utilized for large-scale multiplication of P. bourneae by tissue culture.

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<i>In Vitro</i> Micropropagation of a Valuable Medicinal Plant, <i>Plectranthus amboinicus</i>

Cited by 19 publications

References 6 publications

In vitro plant tissue culture: means for production of biological active compounds

In vitro plant tissue culture: means for production of biological active compounds

In VitroMicropropagation ofAloe adigratanaReynolds Using Offshoot Cuttings

Mass propagation of Plectranthus bourneae Gamble through indirect organogenesis from leaf and internode explants

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