<i>In vitro</i> evaluation of antioxidant and antibacterial activities of extracts of <i>Hertia cheirifolia’s</i> leaves

Abstract

In this study, our objective is to investigate the antioxidant activity by the means of two methods: the β-carotene bleaching method and DPPH assay as well as testing the antibacterial activity by the Agar-well diffusion method, of the extracts (EEp, EDm, EMe and EAq) from the sheets of Hertia cheirifolia. The quantitative analysis are showed that the highest content of total phenolic was concentrated in the methanolic extract with 30.33 ± 2.82 μg EAG/mg of extracts, in the second level the EAq extract and EDm extract with 25.92 ± 7.19 μg EAG/mg of extracts and 21.25 ± 1.76 μg EAG/mg of extracts respectively. The content of polyphenols was determined specrophotometrically and showed the presence of these compounds in all extracts. The analysis by TLC revealed the presence of quercetin in the EMe extract of this plant. In the β-carotene bleaching test, the EMe of this plant displayed highest antioxidant activity (72.97%), than in the DPPH assay with a radical-scavenging activity (72.74%). Our results of the antibacterial activity showed the inefficiency of the whole extracts against most of the tested strains.

Protective activity of Hertia cheirifolia extracts against DNA damage, lipid peroxidation and protein oxidation

“…Previous study also demonstrated that the methanol extract from the same plant had a strong ability to act as antiradical (Bousselsela et al. 2012 ). In addition, both extracts exhibited chelating capacity, and the activity of the aqueous extract was unexpectedly better than that of methanol extract; in perfect contrast to those found for free radical scavenging.…”

GC-MS analysis, phytochemical composition of Hertia cheirifolia L. essential oil with pharmacological assessments: antioxidant, antibacterial, and antifungal activities

“…22,49 The presence of hydroxylated chemicals, such as monoterpenes (α-pinene, camphene, sabinene, limonene, linalool, and verbenol) and sesquiterpenes (germacrene D, α-muurolene, and spathulenol) in the sample could account for the potent inhibitory ability of the essential oil. 50–53 Compared with the results of previous studies on the same plant, 11,18,21,22,25 we note that there is a difference in the results obtained. However, according to previous studies, these differences in antioxidant capacity could be due to several factors, including the molecular nature of bioactive compounds.…”

“…17 In fact, literature studies indicate that H. cheirifolia possesses biological activities. [18][19][20][21][22][23][24][25] Moreover, it is recommended for use as a mosquito control since it is successful against larval stages. 26 To the best of our knowledge, however, no research has investigated the phytochemical constituents and pharmacological properties of the EO (essential oil) found in the aerial parts of H. cheirifolia growing in the Aures area (Algeria), and, in particular, no prior studies have looked at the antifungal potential of the EO of this species, and its association with the mycelial growth of FOL.…”

Chemical composition, antimicrobial activity and chromosome number of Hertia cheirifolia L. from Algeria

“…The methanolic and chloroformic extracts of the aerial parts of H. pallens contain a large amount of sesquiterpenes [1]. The methanolic extract of H. cheirifolia has significant antioxidant activity [12][13][14][15][16].…”

“…The methanolic extracts of H. cheirifolia have moderate antibacterial activity [15][16][26][27], whereas Bousselsela et al [13] reports that oils of this species are ineffective against most bacterial strains tested. The essential oil of H. maroccana is considered moderately active against gram-negative and those of grampositive [9].…”