&amp;lt;i&amp;gt;Bacillus subtilis Strains&amp;lt;/i&amp;gt; with Antifungal Activity against the Phytopathogenic Fungi

Mardanova, Ayslu Mirkasimovna; Hadieva, Guzel Fanisovna; Lutfullin, Marat; Khilyas, Irina V.; Миннуллина, Л. Ф.; Gilyazeva, Adelya Gadelevna; Bogomolnaya, Lidiya Mikhailovna; Шарипова, М. Р.

doi:10.4236/as.2017.81001

Cited by 54 publications

(44 citation statements)

References 40 publications

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“…alone (kumari et al, 2017). Many B. subtilis strains have been reported for their effective biocontrol activity by producing antifungal compounds (Silo-Suh et al, 1994;Souto et al, 2004;Cazorla et al, 2007;Islam et al, 2012 andMardanova et al, 2017).…”

Section: Screening Of Bacterial Isolates For Antagonistic Activity Agmentioning

confidence: 99%

Isolation, screening and growth optimization of antagonistic Bacillus subtilis MS21 from Thengapattanam estuary against plant fungal pathogens

Anjhana¹,

Sasikala²

2017

Int. J. Adv. Res. Biol. Sci.

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In the present work marine sediment samples were collected from Thengapattanam estuary on the south west coast of India for the isolation and screening of bacteria for biocidal potency. The density of the bacterial load in the sediment samples was ranged from 5.43x10 4 to 2.8x10 6 CFU/g. Screening of bacterial isolates for antagonistic activity revealed that the isolate MS21 had the highest zone of clearance among against all the 10 plant fungal pathogens tested. Among 10 fungal pathogens, the highest antagonistic activity was observed against Gleosporium gleosporioide (36mm) and the lowest against Colletotrichum lamella (11mm). The potential isolate was identified as Bacillus subtilis using biochemical methods and it was designated as Bacillus subtilis MS21. Effect of various physicochemical parameters on growth of B. subtilis MS21 showed that 36 hrs of incubation period, 150 rpm agitation, pH-8.0, temperature-35 o C, salinity-1.0%, 2% sucrose as carbon source and 1% beef extract as nitrogen source were found to be the ideal conditions for maximum growth. Maximum growth of 1.92 OD was observed in mass scale culture with the optimized ideal conditions in the shake flask.

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Section: Screening Of Bacterial Isolates For Antagonistic Activity Agmentioning

confidence: 99%

Isolation, screening and growth optimization of antagonistic Bacillus subtilis MS21 from Thengapattanam estuary against plant fungal pathogens

Anjhana¹,

Sasikala²

2017

Int. J. Adv. Res. Biol. Sci.

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“…In this study, the strongest and broadest antagonistic activity against all tested fungi was exhibited by isolates of B. subtilis. High genetic heterogeneity of different Bacillus species, particularly B. subtilis allows to suggest that search and identification of new strains from different sources may expand the number of practically important strains and improve our understanding of mechanisms involved in antagonistic interactions (Mardanova et al 2017).…”

Section: Resultsmentioning

confidence: 99%

Antifungal activity of indigenous Bacillus spp. isolated from soil

Bjelić

Ignjatov

Marinković

et al. 2017

Zb Mat srp prir nauk

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Biocontrol using plant growth-promoting rhizobacteria (PGPR) represents an alternative approach to disease management, since PGPR are known to promote growth and reduce diseases in various crops. Among the different PGPR, members of the genus Bacillus are prefered for most biotechnological uses due to their capability to form extremely resistant spores and produce a wide variety of metabolites with antimicrobial activity. The objective of this research was to identify antagonistic bacteria for management of the plant diseases. Eleven isolates of Bacillus spp. were obtained from the soil samples collected from different localities in the Province of Vojvodina. The antifungal activity of bacterial isolates against five fungal species was examined using a dual plate assay. Bacillus isolates exhibited the highest antifungal activity against Fusarium proliferatum, Fusarium oxysporum f. sp. cepae and Alternaria padwickii, while they had the least antagonistic effect on Fusarium verticillioides and Fusarium graminearum. Molecular identification showed that effective bacterial isolates were identified as Bacillus safensis (B2), Bacillus pumilus (B3, B11), Bacillus subtilis (B5, B7) and Bacillus megaterium (B8, B9). The highest antagonistic activity was exhibited by isolates B5 (from 39% to 62% reduction in fungal growth) and B7 (from 40% to 71% reduction in fungal growth). These isolates of B. subtilis could be used as potential biocontrol agents of plant diseases.

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“…1), dried at 105°C for 24 h and weighted. Also, to study temperature stability of metabolites with inhibitory activity, yeast cell-free supernatant was autoclaved for 30 min at 121°C and tested (Mardanova et al 2016). The per cent of the weight reduction of A. flavus was calculated.…”

Section: Sem Imagingmentioning

confidence: 99%

The action mechanism and biocontrol potentiality of novel isolates of Saccharomyces cerevisiae against the aflatoxigenic Aspergillus flavus

Abdel-Kareem

Rasmey

Zohri

2019

Letters in Applied Microbiology

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Inhibition of Aspergillus flavus growth and its aflatoxins production using the biocontrol agent Saccharomyces cerevisiae as well as to explore its mode of action was studied. Eight strains of S. cerevisiae strains were able to suppress the growth of A. flavus Z103. The maximum growth inhibition of A. flavus Z103 was obtained by living cells of S. cerevisiae EBF101 and S. cerevisiae 117 with 85 and 83%, respectively. The sporulation inhibition and hyphae deterioration of A. flavus Z103 by S. cerevisiae cells adhesion were observed under SEM; up to 99·8% inhibition of aflatoxins biosynthesis by A. flavus Z103 was resulted when the fungus was treated by autoclaved extracellular crude of S. cerevisiae. Also, the tested strains are potential to produce exo‐chitinase which could be suggested as another mode of action for its antifungal activity. GC‐MS analysis of S. cerevisiae 117 extracellular secondary metabolites revealed the existence of 4‐Hydroxyphenethyl alcohol (46·32%), 4, 4‐Dimethyloxazole (9·14%) and 1,2‐Benzenedicarboxylic acid dioctyl ester (2·8%). Significance and Impact of the Study: The use of Saccharomyces cerevisiae instead of chemical preservatives in fermented food, animal and fish feed and storage cereal grains could encourage the food industry to produce organic food free of chemical additives. Overall, our data suggest the possibility of using S. cerevisiae as an alternative treatment in the food industries to control the dispersion and aflatoxins production by Aspergillus flavus during storage. This method could provide an additional probiotic effect in the digestive tract of consumers after ingestion of the treated food. So, our study clarifies the exact mechanisms responsible for the reduction of the aflatoxin contents by S. cerevisiae

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<i>Bacillus subtilis Strains</i> with Antifungal Activity against the Phytopathogenic Fungi

Cited by 54 publications

References 40 publications

Isolation, screening and growth optimization of antagonistic Bacillus subtilis MS21 from Thengapattanam estuary against plant fungal pathogens

Isolation, screening and growth optimization of antagonistic Bacillus subtilis MS21 from Thengapattanam estuary against plant fungal pathogens

Antifungal activity of indigenous Bacillus spp. isolated from soil

The action mechanism and biocontrol potentiality of novel isolates of Saccharomyces cerevisiae against the aflatoxigenic Aspergillus flavus

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