&amp;cestchinlong;Visibly stressed: the role of eIF2, TIA-1, and stress granules in protein translation

Anderson, Paul J.; Kedersha, Nancy

doi:10.1379/1466-1268(2002)007<0213:vstroe>2.0.co;2

Cited by 237 publications

(217 citation statements)

References 43 publications

Supporting

Mentioning

212

Contrasting

Order By: Relevance

“…Under stressful conditions, TIA or TIAR proteins dramatically change their subcellular distribution to associate with SGs or P-bodies. 19 In higher plants, proteins of the oligouridylate-binding protein 1 family (UBP1s) are known as TIA-1/TIAR-related proteins, which were found to bind to a polyuridylate tract to enhance intron recognition, splicing, and mRNA accumulation. UBP1s are assembled into SGs under various stressful conditions.…”

Section: Discussionmentioning

confidence: 99%

Vascular plant one-zinc-finger protein 2 is localized both to the nucleus and stress granules under heat stress in Arabidopsis

Koguchi

Yamasaki

Hirano

et al. 2017

Plant Signaling & Behavior

View full text Add to dashboard Cite

VASCULAR PLANT ONE-ZINC FINGER (VOZ)1/and VOZ2 have an ability to bind to the specific cis-element in the AVP1 promoter of Arabidopsis, which function on the PhyB-dependent flowering and possibly in various stress responses as potential transcription factors, although nuclear localization of VOZ proteins is still unclear. In this study, we found that VOZ2 is dispersed throughout the cytoplasm under normal growth conditions, whereas VOZ2 is transferred not only to the nucleus but also to the cytoplasmic foci under heat stress conditions. The VOZ2 foci predominantly co-localized with a marker of stress granules (SGs), which were cytoplasmic granular structures for mRNA storage and decay under abiotic stress conditions. We also demonstrated that GFP-VOZ2 with a nuclear localization signal was rapidly degraded via the ubiquitin/proteasome pathway under the heat stress conditions. Also, stress-related expression of DREB2A in the voz1voz2 mutant was significantly upregulated by heat stress as compared with that in the wild-type Arabidopsis. Our results suggest that VOZ2 is localized to SGs and nucleus under heat stress conditions, and functions as a transcriptional repressor of DREB2A in Arabidopsis.

show abstract

Section: Discussionmentioning

confidence: 99%

Vascular plant one-zinc-finger protein 2 is localized both to the nucleus and stress granules under heat stress in Arabidopsis

Koguchi

Yamasaki

Hirano

et al. 2017

Plant Signaling & Behavior

View full text Add to dashboard Cite

show abstract

“…However, TIA-1, which is essential for SG assembly (for review, see Anderson and Kedersha, 2002b) and one of its key components, displayed an altered localization in the respective cells. This finding is fairly interesting and clearly deserves further investigations.…”

Section: Discussionmentioning

confidence: 99%

Ataxin-2 Interacts with the DEAD/H-Box RNA Helicase DDX6 and Interferes with P-Bodies and Stress Granules

Nonhoff

Ralser

Welzel

et al. 2007

MBoC

309

320

View full text Add to dashboard Cite

Tight control of translation is fundamental for eukaryotic cells, and deregulation of proteins implicated contributes to numerous human diseases. The neurodegenerative disorder spinocerebellar ataxia type 2 is caused by a trinucleotide expansion in the SCA2 gene encoding a lengthened polyglutamine stretch in the gene product ataxin-2, which seems to be implicated in cellular RNA-processing pathways and translational regulation. Here, we substantiate a function of ataxin-2 in such pathways by demonstrating that ataxin-2 interacts with the DEAD/H-box RNA helicase DDX6, a component of P-bodies and stress granules, representing cellular structures of mRNA triage. We discovered that altered ataxin-2 levels interfere with the assembly of stress granules and cellular P-body structures. Moreover, ataxin-2 regulates the intracellular concentration of its interaction partner, the poly(A)-binding protein, another stress granule component and a key factor for translational control. Thus, our data imply that the cellular ataxin-2 concentration is important for the assembly of stress granules and P-bodies, which are main compartments for regulating and controlling mRNA degradation, stability, and translation. INTRODUCTIONAn essential step in the control of global gene expression in eukaryotes is the regulation of mRNA translation and degradation. Shortening of the poly(A) tail is the initial step to trigger mRNA for decay in two major mRNA degradation pathways in eukaryotic cells (Bernstein and Ross, 1989;Peltz et al., 1991;Decker and Parker, 1994;Beelman and Parker, 1995). In one pathway, the deadenylated mRNA is degraded by a cytoplasmic protein complex, the exosome, consisting of a number of exonucleases with 3Ј-5Ј activity (Butler, 2002). In the other pathway, shortening of the poly(A) tail leads to the removal of the cap structure of the mRNA by the decapping proteins DCP1 and DCP2 facilitating 5Ј-3Ј degradation of the mRNA through the exoribonuclease XRN1 (Beelman and Parker, 1995;Butler, 2002). These proteins colocalize in discrete cytoplasmic foci in mammalian cells, termed processing bodies or P-bodies (also known as DCP1-or GW182-bodies), indicating that mRNA decay is restricted to distinct cytoplasmic compartments in mammalian cells (van Dijk et al., 2002;Cougot et al., 2004). The human protein CCR4, which is involved in mRNA deadenylation, the decapping stimulating LSm proteins LSm1-7, the DEAD/Hbox RNA helicase DDX6 (also known as RCK/p54), GW182, and Ge-1 are components of P-bodies (Bouveret et al., 2000;Eystathioy et al., 2002;Ingelfinger et al., 2002; LykkeAndersen, 2002;Cougot et al., 2004;Yu et al., 2005). Moreover, the RNA-associated protein 55, hEDC3, Hedls as well as factors of the RISC complex localize to P-bodies in mammalian cells (Fenger-Gron et al., 2005;Liu et al., 2005;Sen and Blau, 2005;Yang et al., 2006). P-bodies represent dynamic structures that are in an equilibrium with polysomes and contain nontranslating mRNA . Remarkably, recent work demonstrated that mRNA molecules entering P-bodies can a...

show abstract

“…GCN2 is activated by conditions of amino acid starvation and regulates protein synthesis through phosphorylation of eukaryotic translation initiation factor 2α (eIF2α). The phosphorylation of eIF2α shuts down translation of mRNA and polysome formation and induces the formation of stress granules, which are small cytoplasmic vesicles that contain untranslated mRNA (56). Despite its key role in nutritional sensing and the stress response, GCN2's role in mediating immune response is poorly understood.…”

Section: Vaccines For Which There Are No Knownmentioning

confidence: 99%

“…The metabolic system represents one of the most ancient homeostatic systems that are capable of responding to the most primal of environmental perturbations: changes in nutrient or oxygen conditions or other such perturbations. Mammals have evolved sensors of various such environmental stress signals-for example GCN2, protein kinase R-like endoplasmic reticulum kinase, and heme-regulated inhibitor kinasethat can sense such changes in environmental conditions and orchestrate responses like the integrated stress response (56). Subsequently, in evolution when cells evolved pathogensensing mechanisms, it is possible that some of the sensors and mechanisms that had evolved to detect metabolic changes were co-opted into the pathogen-sensing mechanism.…”

Section: Vaccines For Which There Are No Knownmentioning

confidence: 99%

Systems vaccinology: Probing humanity’s diverse immune systems with vaccines

Pulendran

2014

Proc. Natl. Acad. Sci. U.S.A.

169

123

View full text Add to dashboard Cite

Homo sapiens are genetically diverse, but dramatic demographic and socioeconomic changes during the past century have created further diversification with respect to age, nutritional status, and the incidence of associated chronic inflammatory disorders and chronic infections. These shifting demographics pose new challenges for vaccination, as emerging evidence suggests that age, the metabolic state, and chronic infections can exert major influences on the immune system. Thus, a key public health challenge is learning how to reprogram suboptimal immune systems to induce effective vaccine immunity. Recent advances have applied systems biological analysis to define molecular signatures induced early after vaccination that correlate with and predict the later adaptive immune responses in humans. Such "systems vaccinology" approaches offer an integrated picture of the molecular networks driving vaccine immunity, and are beginning to yield novel insights about the immune system. Here we discuss the promise of systems vaccinology in probing humanity's diverse immune systems, and in delineating the impact of genes, the environment, and the microbiome on protective immunity induced by vaccination. Such insights will be critical in reengineering suboptimal immune systems in immunocompromised populations.

show abstract

&cestchinlong;Visibly stressed: the role of eIF2, TIA-1, and stress granules in protein translation

Cited by 237 publications

References 43 publications

Vascular plant one-zinc-finger protein 2 is localized both to the nucleus and stress granules under heat stress in Arabidopsis

Vascular plant one-zinc-finger protein 2 is localized both to the nucleus and stress granules under heat stress in Arabidopsis

Ataxin-2 Interacts with the DEAD/H-Box RNA Helicase DDX6 and Interferes with P-Bodies and Stress Granules

Systems vaccinology: Probing humanity’s diverse immune systems with vaccines

Contact Info

Product

Resources

About