AMP-activated protein kinase is a biomarker of energetic status in freshwater mussels exposed to municipal effluents

Goodchild, Christopher G.; Frederich, Markus; Zeeman, Stephan I.

doi:10.1016/j.scitotenv.2015.01.065

Cited by 23 publications

(14 citation statements)

References 70 publications

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“…In our case, we used AMPK because it has been mechanistically identified as an upstream regulator of macromolecule metabolism and is directly activated by slight decreases in ATP concentrations . Therefore, we propose that AMPK may be a valuable energetic biomarker for use in ecotoxicological studies because it is sensitive to low concentrations of pollutants in both controlled and field scenarios (see Goodchild et al, 2015), though further comparative studies are needed to evaluate which methods are most informative.…”

Section: Discussionmentioning

confidence: 99%

“…Alien RNA (Agilent, La Jolla, CA, USA) was added as an external control for alerting reverse transcription inhibition and 10 normalizing PCR efficiency (Huggett et al, 2005). Gene targets were amplified with primers developed by Goodchild et al (2015 ; Table 1) and an Agilent Brilliant SYBR Green qPCR Kit (Agilent, La Jolla, CA, USA) on a Stratagene MX3005s instrument. After 40 cycles with an annealing temperature of 53º C for AMPKγ and HSP70, or 55º C for SOD and GST, a melting curve analysis confirmed that only one DNA product was amplified per gene target.…”

Section: Mrna Expressionmentioning

confidence: 99%

“…Although the structure of AMPK has not been validated in mussels, AMPK is highly conserved between different eukaryotes (Hardie, 2007), and we have therefore assumed this kinase has a heterotrimeric structure in mussels as well (Guevelou et al, 2013;Jost et al, 2015). Recently, we found evidence for AMPK as a sensitive energetic biomarker to municipal effluent exposure in situ (Goodchild et al, 2015), but it is unknown whether this is associated with effects at higher levels of biological organization.…”

Section: Introductionmentioning

confidence: 98%

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Is altered behavior linked to cellular energy regulation in a freshwater mussel (Elliptio complanata) exposed to triclosan?

Goodchild

Frederich

Zeeman

2016

Comparative Biochemistry and Physiology Part C: Toxicology &

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Environmental stress may alter the bioenergetic balance of organisms by resulting in greater energy investment into detoxification processes, which diverts energy from other biological functions. Here, we examine responses to triclosan (TCS) exposure in a freshwater mussel across multiple biological levels: behavioral (e.g., burrowing and movement activity), organismal (e.g., metabolic rate and heart rate), and subcellular (e.g., gene expression and protein abundance/activity). At the subcellular level, we employed both energetic (i.e., AMP-activated protein kinase (AMPK)) and traditional (i.e., heat shock protein (HSP70), superoxide dismutase (SOD), glutathione-S-transferase (GST)) biomarkers. We found a significant reduction in burrowing and movement behaviors, a 1.8-fold increase in total-AMPK protein abundance, and a 2.8-fold increase in AMPK activity after 21d. GST activity increased after 4d, but not after 21d. Our findings suggest that TCS exposure results in an energetic tradeoff between detoxification at the cellular level and whole-animal activity.

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Section: Discussionmentioning

confidence: 99%

Section: Mrna Expressionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Is altered behavior linked to cellular energy regulation in a freshwater mussel (Elliptio complanata) exposed to triclosan?

Goodchild

Frederich

Zeeman

2016

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…Gust et al (2013) detected immunotoxic effects (at functional and genomic level) on the fresh water mollusc Lymnaea stagnalis exposed to an urban effluent containing estrogenic and pharmaceutical compounds. Moreover, Goodchild et al (2015) detected a negative impact on the energy metabolism of the freshwater mussel Elliptio complanata exposed to municipal effluents, namely a decrease on AMP-activated protein kinase and an increase in the antioxidant system (SOD and GST) indicating increase in energy consumption.…”

Section: Discussionmentioning

confidence: 99%

Proteomic changes in Corbicula fluminea exposed to wastewater from a psychiatric hospital

Bebianno

Sroda

Gomes

et al. 2015

Environ Sci Pollut Res

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The increase use of pharmaceutical compounds in veterinary practice and human population results in the ubiquitous presence of these compounds in aquatic ecosystems. Because pharmaceuticals are highly bioactive, there is concern about their toxicological effects in aquatic organisms. Therefore, the aim of this study was to assess the effects of an effluent from a psychiatric hospital (containing a complex mixture of 25 pharmaceutical compounds from eleven therapeutic classes) on the freshwater clam Corbicula fluminea using a proteomic approach. The exposure of C. fluminea to this complex effluent containing anxiolytics, analgesics, lipid regulators, beta blockers, antidepressants, antiepileptics, antihistamines, antihypertensives, antiplatelets and antiarrhythmics induced protein changes after 1 day of exposure in clam gills and digestive gland more evident in the digestive gland. These changes included increase in the abundance of proteins associated with structural (actin and tubulin), cellular functions (calreticulin, proliferating cell nuclear antigen (PCNA), T complex protein 1 (TCP1)) and metabolism (aldehyde dehydrogenase (ALDH), alcohol dehydrogenase, 6 phosphogluconate dehydrogenase). Results from this study indicate that calreticulin, PCNA, ALDH and alcohol dehydrogenase in the digestive gland and T complex protein 1 (TCP1)) and 6 phosphogluconate dehydrogenase in the gills represent useful biomarkers for the ecotoxicological characterization of psychiatric hospital effluents in this species.

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“…Municipal wastewater effluents. Goodchild et al, (2015) investigated sublethal effects of exposure to wastewater treatment facility effluent in freshwater mussels in situ, thereby capturing ecologically relevant exposure conditions. This is the first study to use energetic biomarker AMP-activated protein kinase (AMPK) to evaluate the effects of contamination in situ, and our results suggest that energetic biomarkers, like AMPK, complement traditional biomarkers and may help establish functional links between cellular and whole-animal effects.…”

Section: Wastewatermentioning

confidence: 99%

Effects of Pollution on Freshwater Organisms

Choudri

Baawain

2016

Water Environment Research

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This review includes works published in the general scientific literature during 2015 on the effects of anthropogenic pollutants on freshwater organisms. It begins with two broad sections: research reviews and broad field studies and surveys. This is followed by reviews of research categorized in sections to reflect the pollutant class. These sections include wastewater, stormwater and non-point source pollution, nutrients, sediment cap materials and suspended clays, botanical extracts, surfactants, metals, persistent organic pollutants, pharmaceuticals, endocrine disruptors, pesticides, petroleum hydrocarbons and polycyclic aromatic hydrocarbons (PAHs), ionic liquids, and nanomaterials. The final section includes works describing innovations in the field of freshwater pollution research.

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AMP-activated protein kinase is a biomarker of energetic status in freshwater mussels exposed to municipal effluents

Cited by 23 publications

References 70 publications

Is altered behavior linked to cellular energy regulation in a freshwater mussel (Elliptio complanata) exposed to triclosan?

Is altered behavior linked to cellular energy regulation in a freshwater mussel (Elliptio complanata) exposed to triclosan?

Proteomic changes in Corbicula fluminea exposed to wastewater from a psychiatric hospital

Effects of Pollution on Freshwater Organisms

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