“…Cell culture: Rat A7r5 ASM cells (ATCC, Manassas, VA) were cultured under sterile conditions in Dulbecco's Modified Eagles Medium (DMEM) supplemented with fetal bovine serum (FBS, 0.2-10%), 2 mM L-glutamine, a 1:500 dilution of 50 µg/mL Primocin (Invivogen) at 37°C in 95% air/5% CO 2 and were serially split and used through passage 6 as described [11,13,22].…”