&amp;#969;3 and &amp;#969;6 CYP450 Eicosanoid Derivatives: Key Lipid Mediators in the Regulation of Pulmonary Hypertension

Morin, Caroline; Fortin, Samuel; Guibert, Christelle; Rousseau, Éric

doi:10.5772/26561

Cited by 3 publications

(6 citation statements)

References 111 publications

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“…Studies have shown that DHA in the form of monoacylglyceride increases the bioavailability of DHA compared to triglycerides [ 31 ]. Our previous studies have shown that DHA in monoacylglyceride form displayed an increased bioavailability over free fatty acid in both cells and animal models [ 1 , 4 , 32 , 33 ]. In this pilot study, the supplementation was well tolerated by CF patients, despite the omega-3 PUFA supplementation being administered at high doses; MAG-DHA did not modify the body mass index (BMI), platelets and liver enzymes.…”

Section: Discussionmentioning

confidence: 99%

“…Fatty acid composition of erythrocytes using gas chromatography/flame ionization detector (GC/FID): Fatty acid compositions of erythrocytes derived from CF patients were measured using a modified direct transesterification method in which toluene was used instead of benzene and acetyl chloride was replaced by sulfuric acid [ 1 , 2 ]. Fatty acids were chromatographed as methyl esters on a 60-m fused silica column with an internal diameter of 0.25 mm.…”

Section: Methodsmentioning

confidence: 99%

“…A new marine omega-3 compound, a docosahexaenoic acid (DHA) sn1-monoacylglyceride (MAG-DHA), was synthesized in order to evaluate its anti-inflammatory properties in Cystic Fibrosis (CF) patients. This compound showed better absorption of DHA compared with either DHA-triglyceride or DHA-ethyl ester [ 1 ]. In addition, its anti-inflammatory effect on the airways has been demonstrated on 2 in vitro models related to CF [ 2 , 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

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The Efficacy of MAG-DHA for Correcting AA/DHA Imbalance of Cystic Fibrosis Patients

Morin¹,

Cantin

Vézina

et al. 2018

Marine Drugs

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Omega-3 polyunsaturated fatty acid (n-3 PUFA) supplementations are thought to improve essential fatty acid deficiency (EFAD) as well as reduce inflammation in Cystic Fibrosis (CF), but their effectiveness in clinical studies remains unknown. The aim of the study was to determine how the medical food containing docosahexaenoic acid monoglyceride (MAG-DHA) influenced erythrocyte fatty acid profiles and the expression levels of inflammatory circulating mediators. We conducted a randomized, double blind, pilot trial including fifteen outpatients with Cystic Fibrosis, ages 18–48. The patients were divided into 2 groups and received MAG-DHA or a placebo (sunflower oil) for 60 days. Patients took 8 × 625 mg MAG-DHA softgels or 8 × 625 mg placebo softgels every day at bedtime for 60 days. Lipid analyses revealed that MAG-DHA increased docosahexaenoic acid (DHA) levels and decrease arachidonic acid (AA) ratio (AA/DHA) in erythrocytes of CF patients following 1 month of daily supplementation. Data also revealed a reduction in plasma human leukocyte elastase (pHLE) complexes and interleukin-6 (IL-6) expression levels in blood samples of MAG-DHA supplemented CF patients. This pilot study indicates that MAG-DHA supplementation corrects erythrocyte AA/DHA imbalance and may exert anti-inflammatory properties through the reduction of pHLE complexes and IL6 in blood samples of CF patients. Trial registration: Pro-resolving Effect of MAG-DHA in Cystic Fibrosis (PREMDIC), NCT02518672.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Efficacy of MAG-DHA for Correcting AA/DHA Imbalance of Cystic Fibrosis Patients

Morin¹,

Cantin

Vézina

et al. 2018

Marine Drugs

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“…An alternative explanation would be that monoacylglycerides have more potent effects on mitochondrial respiration than other forms of n-3 PUFAs. Indeed, oral supplementation of n-3 PUFA monoacylglycerides have been shown to increase the plasma concentration and bioavailability of these n-3 PUFAs in rodents compared to other forms (Morin et al 2011), but this hypothesis has to be properly tested before being validated.…”

Section: Discussionmentioning

confidence: 99%

Omega-3 Monoacylglyceride Effects on Longevity, Mitochondrial Metabolism and Oxidative Stress: Insights from <em>Drosophila melanogaster</em>

Champigny

Cormier

Simard

et al. 2018

Preprint

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During the last decade, essential polyunsaturated fatty acids (PUFAs) such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) derived from marine sources have been investigated as nonpharmacological dietary supplements to improve different pathological conditions, as well as aging. The aim of this study was to determine the effects of dietary n-3 PUFA monoacylglycerides (MAG, both EPA and DHA) on the mitochondrial metabolism and oxidative stress of a short-lifespan model, Drosophila melanogaster, sampled at five different ages. Our results showed that diets supplemented with MAG-EPA and MAG-DHA increased median lifespan by 14.6% and decreased mitochondrial proton leak resulting in an increase of mitochondrial coupling. The flies fed on MAG-EPA also had higher electron transport system capacity and mitochondrial oxidative capacities. Moreover, both n-3 PUFAs delayed the occurrence of lipid peroxidation, but only flies fed the MAG-EPA diet showed maintenance of superoxide dismutase activity during aging. Our study therefore highlights the potential of n-3 PUFA monoacylglycerides as nutraceutical compounds to delay the onset of senescence by acting directly or indirectly on the mitochondrial metabolism, and suggests that Drosophila could be a relevant model for the study of the fundamental mechanisms linking the effects of n-3 PUFAs to aging.

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“…Although these compounds are a heterogenous mixture of mono- and diacylglycerides, they were chosen in our study based on their high contents in monoacyglycerides (more than 40%). These compounds have been shown to increase the plasma concentration and bioavailability of both EPA and DHA n-3 PUFAs in rodents compared to other forms [ 22 ] and have never been tested in Drosophila. This short-lifespan model has recently emerged as a suitable model to understand the fundamental mechanisms that control metabolism [ 23 , 24 , 25 , 26 , 27 ] and does not possess the Δ 5 and Δ 6 desaturases which participate in the conversion of ALA to EPA and of EPA to DHA [ 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

Omega-3 Monoacylglyceride Effects on Longevity, Mitochondrial Metabolism and Oxidative Stress: Insights from Drosophila melanogaster

et al. 2018

Self Cite

View full text Add to dashboard Cite

During the last decade, essential polyunsaturated fatty acids (PUFAs) such as eicosatetraenoic acid (EPA) and docosahexaenoic acid (DHA) derived from marine sources have been investigated as nonpharmacological dietary supplements to improve different pathological conditions, as well as aging. The aim of this study was to determine the effects of dietary n-3 PUFA monoacylglycerides (MAG, both EPA and DHA) on the mitochondrial metabolism and oxidative stress of a short-lifespan model, Drosophila melanogaster, sampled at five different ages. Our results showed that diets supplemented with MAG-EPA and MAG-DHA increased median lifespan by 14.6% and decreased mitochondrial proton leak resulting in an increase of mitochondrial coupling. The flies fed on MAG-EPA also had higher electron transport system capacity and mitochondrial oxidative capacities. Moreover, both n-3 PUFAs delayed the occurrence of lipid peroxidation but only flies fed the MAG-EPA diet showed maintenance of superoxide dismutase activity during aging. Our study therefore highlights the potential of n-3 PUFA monoacylglycerides as nutraceutical compounds to delay the onset of senescence by acting directly or indirectly on the mitochondrial metabolism and suggests that Drosophila could be a relevant model for the study of the fundamental mechanisms linking the effects of n-3 PUFAs to aging.

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ω3 and ω6 CYP450 Eicosanoid Derivatives: Key Lipid Mediators in the Regulation of Pulmonary Hypertension

Cited by 3 publications

References 111 publications

The Efficacy of MAG-DHA for Correcting AA/DHA Imbalance of Cystic Fibrosis Patients

The Efficacy of MAG-DHA for Correcting AA/DHA Imbalance of Cystic Fibrosis Patients

Omega-3 Monoacylglyceride Effects on Longevity, Mitochondrial Metabolism and Oxidative Stress: Insights from <em>Drosophila melanogaster</em>

Omega-3 Monoacylglyceride Effects on Longevity, Mitochondrial Metabolism and Oxidative Stress: Insights from Drosophila melanogaster

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