Amorphus suaedae sp. nov., isolated from the root of a tidal flat plant, Suaeda maritima

Abstract

Amorphus suaedae sp. nov., isolated from the root of a tidal flat plant, Suaeda maritima A novel bacterial strain, YC6899 T , was isolated from the root of Suaedae maritima growing on a tidal flat of Namhae Island, Korea. Cells were Gram-reaction-negative, rod-shaped, non-motile, slightly halophilic and heterotrophic. Strain YC6899 T grew optimally at a salinity of 2-4 %, at 25-37 6C and at pH 6.5-8.0. Phylogenetic analysis of 16S rRNA gene sequences demonstrated that strain YC6899 T was closely related to Amorphus orientalis YIM D10 T (96.1 % similarity) and Amorphus coralli RS.Sph.026 T (95.9 %). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, unknown aminolipids, an unknown aminophospholipid, an unknown aminoglycolipid, unknown glycolipids and unknown lipids. The major fatty acids of strain YC6899 T were C 19 : 0 cyclo v8c and C 18 : 1 v7c. The G+C content of the genomic DNA was 61.3 mol%. Strain YC6899 T contained ubiquinone-10 (Q-10) as the major respiratory quinone system. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain YC6899 T represents a novel species within the genus Amorphus, for which the name Amorphus suaedae sp. nov. is proposed. The type strain is YC6899 T (5KACC 14912 T 5NBRC 107845 T ).