Amorphous Calcium Carbonate Granules Form Within an Intracellular Compartment in Calcifying Cyanobacteria

Blondeau, Marine; Sachse, Martin; Boulogne, Claire; Gillet, Cynthia; Guigner, Jean‐Michel; Skouri‐Panet, Fériel; Poinsot, Mélanie; Férard, Céline; Miot, Jennyfer; Benzerara, Karim

doi:10.3389/fmicb.2018.01768

Cited by 41 publications

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“…[ 149 ] The PNCs for calcium carbonate are held together by the ionic bond between a calcium cation and carbonate anion instead of covalent linkage. [ 150 ] The PNCs in microbe‐mediated calcium carbonate precipitation produce amorphous calcium carbonate (ACC) [ 151 ] and in microbe‐mediated calcium phosphate precipitation produce amorphous calcium phosphate (ACP). [ 152 ] Extracellular organic material in the EPS matrix can serve as nucleation sites to facilitate precipitation of ACC.…”

Section: Mechanisms Of Microbe‐mediated Mineralizationmentioning

confidence: 99%

Microbe‐Mediated Extracellular and Intracellular Mineralization: Environmental, Industrial, and Biotechnological Applications

et al. 2020

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Bacteria play an important role in the calcification process of natural minerals and within organisms ( Table 1). It is Microbe-mediated mineralization is ubiquitous in nature, involving bacteria, fungi, viruses, and algae. These mineralization processes comprise calcification, silicification, and iron mineralization. The mechanisms for mineral formation include extracellular and intracellular biomineralization. The mineral precipitating capability of microbes is often harnessed for green synthesis of metal nanoparticles, which are relatively less toxic compared with those synthesized through physical or chemical methods. Microbe-mediated mineralization has important applications ranging from pollutant removal and nonreactive carriers, to other industrial and biomedical applications. Herein, the different types of microbe-mediated biomineralization that occur in nature, their mechanisms, as well as their applications are elucidated to create a backdrop for future research.

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Section: Mechanisms Of Microbe‐mediated Mineralizationmentioning

confidence: 99%

Microbe‐Mediated Extracellular and Intracellular Mineralization: Environmental, Industrial, and Biotechnological Applications

et al. 2020

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“…The outflux of Ca is usually assumed to occur actively through transporters. Outflux may proceed from the cytosol toward the extracellular solution or from the cytosol toward an intracellular compartment as the one delimitating intracellular ACC (Blondeau, Sachse, et al., ). Here, we identified in all ACC+ cyanobacteria at least one gene coding for a Ca 2+ /H + transporter and at least one gene coding for a putative calcium exchanger of the UPF0016 family.…”

Section: Discussionmentioning

confidence: 99%

“…PCC 6312 and the two other ACC+ strains: While Synechococcus sp . PCC 6312 forms intracellular ACC granules in connection with cell division and between the cytoplasmic membrane and the outermost thylakoids, the two other strains form ACC granules in the center of the cells with no apparent connection with cell division (Benzerara et al., ; Blondeau, Sachse, et al., ). Overall, intracellular carbonatogenesis likely follows different pathways in the clade of Synechococcus sp.…”

Section: Discussionmentioning

confidence: 99%

“…Blondeau, Sachse, et al. () showed by using cryo‐electron microscopy of vitreous sections that intracellular ACC were systematically enclosed within an envelope which could be a protein shell or a lipid monolayer, suggesting that chemical conditions (e.g., Ca 2+ concentration) within these vesicles might be different from those in the cytosol and more suitable to ACC precipitation.…”

Section: Introductionmentioning

confidence: 99%

“…Based on the size distribution and spatial location of intracellular Ca-carbonates in diverse strains, several nucleation sites have been suggested for these precipitates, including (a) carboxysomes for cyanobacteria showing ACC inclusions throughout their cells or (b) cytoskeletal proteins for cyanobacteria with ACC inclusions located at their septum and their poles . Blondeau, Sachse, et al (2018) showed by using cryo-electron microscopy of vitreous sections that intracellular ACC were systematically enclosed within an envelope which could be a protein shell or a lipid monolayer, suggesting that chemical conditions (e.g., Ca 2+ concentration) within these vesicles might be different from those in the cytosol and more suitable to ACC precipitation. However, differences in ability to manage Ca between cyanobacterial strains forming intracellular ACC and other strains of cyanobacteria are still unclear.…”

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Evidence of high Ca uptake by cyanobacteria forming intracellular CaCO₃ and impact on their growth

et al. 2019

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Several species of cyanobacteria biomineralizing intracellular amorphous calcium carbonates (ACC) were recently discovered. However, the mechanisms involved in this biomineralization process and the determinants discriminating species forming intracellular ACC from those not forming intracellular ACC remain unknown. Recently, it was hypothesized that the intensity of Ca uptake (i.e., how much Ca was scavenged from the extracellular solution) might be a major parameter controlling the capability of a cyanobacterium to form intracellular ACC. Here, we tested this hypothesis by systematically measuring the Ca uptake by a set of 52 cyanobacterial strains cultured in the same growth medium. The results evidenced a dichotomy among cyanobacteria regarding Ca sequestration capabilities, with all strains forming intracellular ACC incorporating significantly more calcium than strains not forming ACC. Moreover, Ca provided at a concentration of 50 μM in BG‐11 was shown to be limiting for the growth of some of the strains forming intracellular ACC, suggesting an overlooked quantitative role of Ca for these strains. All cyanobacteria forming intracellular ACC contained at least one gene coding for a mechanosensitive channel, which might be involved in Ca influx, as well as at least one gene coding for a Ca2+/H+ exchanger and membrane proteins of the UPF0016 family, which might be involved in active Ca transport either from the cytosol to the extracellular solution or the cytosol toward an intracellular compartment. Overall, massive Ca sequestration may have an indirect role by allowing the formation of intracellular ACC. The latter may be beneficial to the growth of the cells as a storage of inorganic C and/or a buffer of intracellular pH. Moreover, high Ca scavenging by cyanobacteria biomineralizing intracellular ACC, a trait shared with endolithic cyanobacteria, suggests that these cyanobacteria should be considered as potentially significant geochemical reservoirs of Ca.

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Indigenous microbial communities as catalysts for early marine cements: An in vitro study

Diaz

Eberli

Weger

2022

The Depositional Record

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Early marine cementation is a fundamental process for many characteristics of carbonates, like the stabilisation of steep slopes. The genesis of early cements is often attributed to physicochemical processes but there is evidence for microbial mediation. To elucidate the role of microbes and associated organic material, in vitro experiments were undertaken in the presence and absence of indigenous microbiota in ooids from Schooner Cays, Bahamas and compared with native grapestones from Joulter Cays, Bahamas. Microscopic examinations by stereomicroscopy, scanning electron microscopy and thin section analysis of in vitro incubations with native flora document rapid grain fusion, resulting in the formation of grapestones within 30-60 days. The initial binding of the grains is primarily facilitated by exudates of extracellular polymeric substances and microbial communities acting as catalysts in the formation of micritic bridges, cements and encrusted aggregates. In vitro grapestones are similar to native grapestones from Joulter Cays with intergranular areas infested with extracellular polymeric substances, microbes, micritic cements, amorphous calcium carbonate nanograins and micritised outer surfaces. These similarities suggest that incubations with native flora follow similar mineralisation mechanisms as in the natural environment. In contrast, sterilised grains remain loose with little crystal formation after 60 days and are devoid of microbes and organic exudates. Owing to the near absence of precipitates, abiotic precipitation is not the driving force promoting early cements. In contrast, grain fusion is microbially mediated via both a passive mechanism, where extracellular polymeric substances and cell surfaces function as templates for crystal nucleation and generation of micritic cements, and through an active mechanism by which biofilm heterotrophs and autotrophs induce chemical alterations of a local environment, facilitating precipitation. This study underscores that microbially mediated cementation can occur at fast rates and that firmground to hardgrounds and slope stabilisation take place shortly after deposition of carbonate grains.

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Amorphous Calcium Carbonate Granules Form Within an Intracellular Compartment in Calcifying Cyanobacteria

Cited by 41 publications

References 63 publications

Microbe‐Mediated Extracellular and Intracellular Mineralization: Environmental, Industrial, and Biotechnological Applications

Microbe‐Mediated Extracellular and Intracellular Mineralization: Environmental, Industrial, and Biotechnological Applications

Evidence of high Ca uptake by cyanobacteria forming intracellular CaCO₃ and impact on their growth

Indigenous microbial communities as catalysts for early marine cements: An in vitro study

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Amorphous Calcium Carbonate Granules Form Within an Intracellular Compartment in Calcifying Cyanobacteria

Cited by 41 publications

References 63 publications

Microbe‐Mediated Extracellular and Intracellular Mineralization: Environmental, Industrial, and Biotechnological Applications

Microbe‐Mediated Extracellular and Intracellular Mineralization: Environmental, Industrial, and Biotechnological Applications

Evidence of high Ca uptake by cyanobacteria forming intracellular CaCO3 and impact on their growth

Indigenous microbial communities as catalysts for early marine cements: An in vitro study

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Evidence of high Ca uptake by cyanobacteria forming intracellular CaCO₃ and impact on their growth