Amoebicidal efficacy of a novel multi-purpose disinfecting solution: First findings

Üstüntürk, Miray; Zeybek, Zuhal

doi:10.1016/j.exppara.2014.05.011

Cited by 15 publications

(10 citation statements)

References 22 publications

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“…However, none of the contact lens disinfectants completely destroyed A. castellanii trophozoites and cyst. These findings are consistent with several recent studies indicating that marketed contact lens disinfectants are ineffective in completely destroying amoebae (Kolar et al, 2015;Padzik et al, 2014;Polat et al, 2007;Ustüntürk and Zeybek, 2014). In particular, there show limited efficacy against cysts.…”

Section: Discussionsupporting

confidence: 82%

Status of the effectiveness of contact lens disinfectants in Malaysia against keratitis-causing pathogens

Abjani

Khan

Jung

et al. 2017

Experimental Parasitology

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The aim of this study was (i) to assess the antimicrobial effects of contact lens disinfecting solutions marketed in Malaysia against common bacterial eye pathogens and as well as eye parasite, Acanthamoeba castellanii, and (ii) to determine whether targeting cyst wall would improve the efficacy of contact lens disinfectants. Using ISO 14729 Stand-Alone Test for disinfecting solutions, bactericidal and amoebicidal assays of six different contact lens solutions including Oxysept ® , AO SEPT PLUS, OPTI-FREE ® pure moist ® , Renu ® fresh™, FreshKon ® CLEAR and COMPLETE RevitaLens™ were performed using Manufacturers Minimum recommended disinfection time (MRDT). The efficacy of contact lens solutions was determined against keratitis-causing microbes, namely: Pseudomonas aeruginosa, Methicillin-resistant Staphylococcus aureus, Streptococcus pyogenes, Streptococcus pneumoniae, and Acanthamoeba castellanii. In addition, using chlorhexidine as an antiamoebic compound and cellulase enzyme to disrupt cyst wall structure, we determined whether combination of both agents can enhance efficacy of marketed contact lens disinfectants against A. castellanii trophozoites and cysts, in vitro. The results revealed that all contact lens disinfectants tested showed potent bactericidal effects exhibiting 100% kill against all bacterial species tested. In contrast, none of the contact lens disinfectants had potent effects against Acanthamoeba cysts viability. When tested against trophozoites, two disinfectants, Oxysept Multipurpose and AO-sept Multipurpose showed partial amoebicidal effects. Using chlorhexidine as an antiamoebic compound and cellulase enzyme to disrupt cyst wall structure, the findings revealed that combination of both agents in contact lens disinfectants abolished viability of A. castellanii cysts and trophozoites. Given the inefficacy of contact lens disinfectants tested in this study, these findings present a significant concern to public health. These findings revealed that targeting cyst wall by using cyst wall degrading molecules in contact lens disinfecting solutions will enhance their efficacy against this devastating eye infection.

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Section: Discussionsupporting

confidence: 82%

Status of the effectiveness of contact lens disinfectants in Malaysia against keratitis-causing pathogens

Abjani

Khan

Jung

et al. 2017

Experimental Parasitology

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“…Unfortunately, many commercial solutions for lens hygiene containing PHMB were ineffective against Acanthamoeba contamination in contact lenses, as demonstrated by a study conducted in Korea [11]. Similar findings have been observed for many multipurpose cleansing solutions in other studies [12][13][14][15][16].…”

Section: Introductionsupporting

confidence: 69%

In Vitro Evaluation of the Combination of Melaleuca alternifolia (Tea Tree) Oil and Dimethyl Sulfoxide (DMSO) against Trophozoites and Cysts of Acanthamoeba Strains. Oxygen Consumption Rate (OCR) Assay as a Method for Drug Screening

et al. 2021

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Ameobae belonging to the genus Acanthamoeba are responsible for the human diseases Acanthamoeba keratitis (AK) and granulomatous amoebic encephalitis (GAE). The treatment of these illnesses is hampered by the existence of a resistance stage (cysts). In an attempt to add new agents that are effective against trophozoites and cysts, tea tree oil (TTO) and dimethyl sulfoxide (DMSO), separately and in combination, were tested In Vitro against two Acanthamoeba isolates, T3 and T4 genotypes. The oxygen consumption rate (OCR) assay was used as a drug screening method, which is to some extent useful in amoebicide drug screening; however, evaluation of lethal effects may be misleading when testing products that promote encystment. Trophozoite viability analysis showed that the effectiveness of the combination of both compounds is higher than when either compound is used alone. Therefore, the TTO alone or TTO + DMSO in combination were an amoebicide, but most of the amoebicidal activity in the combination’s treatments seemed to be caused mainly by the TTO effect. In fact, DMSO alone seems to be a non-amoebicide, triggering encystment. Regarding cytotoxicity, these compounds showed toxicity in human corneal epithelial cells (HCEpiC), even at low concentrations when tested in combination. In conclusion, the use of TTO and DMSO, in combination or alone, cannot be recommended as an alternative for AK treatment until more cytotoxicity and cyst adhesion tests are performed.

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“…While there is interest in incorporating the testing of care solutions against Acanthamoeba for regulatory purposes, there are important issues that need to be solved, such as standardisation of the testing procedure [276,[280][281][282]. Although some care solutions might be effective against Acanthamoeba trophozoites, many are not against the dormant highly resistant cyst stage [277,278,[283][284][285][286][287][288]. The long-term survival and resistance of cysts to disinfectants adds complexity with regards to developing safe and effective contact care disinfecting solutions against Acanthamoeba [289].…”

Section: Disinfectantsmentioning

confidence: 99%

BCLA CLEAR - Contact lens wettability, cleaning, disinfection and interactions with tears

Willcox

Keir

Maseedupally

et al. 2021

Contact Lens and Anterior Eye

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Contact lens materials have undergone significant changes over the past 20 years, particularly with respect to the introduction of silicone hydrogel materials. Whilst this development addressed hypoxic issues, other important areas relating to contact lens success, notably comfort, require further research.Contact lens wettability remains a crucially important part of biocompatibility. Contact lenses can be made more wettable by incorporation of surfactants into blister packs, internal wetting agents, surface treatments or care solutions. However, there remains no clear association between contact lens wettability and comfort, making it challenging to determine the potential for these approaches to be of significant clinical benefit. Most contact lenses are used on a daily wear, reusable basis, which requires them to be disinfected when not worn. The ideal disinfecting solution would also improve comfort during wear. However, balancing these requirements with other factors, including biocompatibility, remains a challenge. Soft lens materials invariably take up and subsequently release certain components of disinfecting solutions onto the ocular surface. This may affect tear film stability and the normal ocular microbiome, and further research is needed in this area to determine whether this has any affect on comfort. Finally, contact lens materials sorb components of the tear film, and these interactions are complex and may change the biochemistry of the tear film, which in turn may affect their comfort.In conclusion, the interaction between lens materials, tear film and disinfection solution plays an important role in the biocompatibility of lenses. However, the exact role and whether this can be altered to improve biocompatibility and comfort during wear remains debatable. This report summarises the best available evidence to examine this complex relationship and the opportunities for practitioners to enhance in-eye comfort of contemporary lenses, along with providing suggestions for areas of study that may provide further information on this topic.

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Amoebicidal efficacy of a novel multi-purpose disinfecting solution: First findings

Cited by 15 publications

References 22 publications

Status of the effectiveness of contact lens disinfectants in Malaysia against keratitis-causing pathogens

Status of the effectiveness of contact lens disinfectants in Malaysia against keratitis-causing pathogens

In Vitro Evaluation of the Combination of Melaleuca alternifolia (Tea Tree) Oil and Dimethyl Sulfoxide (DMSO) against Trophozoites and Cysts of Acanthamoeba Strains. Oxygen Consumption Rate (OCR) Assay as a Method for Drug Screening

BCLA CLEAR - Contact lens wettability, cleaning, disinfection and interactions with tears

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