Amoebicidal activity of curcumin on <i>Entamoeba histolytica</i> trophozoites

Rangel-Castañeda, Itzia Azucena; Pérez-Rangel, Armando; González-Pozos, Sirenia; Carranza-Rosales, Pilar; Charles-Niño, Claudia; Tapia-Pastrana, Gabriela; Ramírez-Herrera, Mario Alberto; Castillo-Romero, Araceli

doi:10.1111/jphp.12867

Cited by 13 publications

(14 citation statements)

References 30 publications

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“…CUR, the active component of turmeric, has received considerable interest as a potential therapeutic agent for the prevention or treatment of various diseases, it possesses a wide range of pharmacological properties, for example anticancer [ 30,31 ] and antiparasitic activity. A recent work shows that CUR reduces growth in E. histolytica [ 10 ] and in G. lamblia. [ 11,32 ] However, although CUR shows excellent pharmacological qualities, its clinical use is limited, due to the low solubility in water [ 12,13 ] ; thus, limiting their bioavailability and poor systemic absorption in the digestive tract, this promotes poor pharmacokinetics in vivo , which affects their pharmacological effect.…”

Section: Discussionmentioning

confidence: 99%

“…Several works have shown that CUR exhibits also anticancer activity, in Caco‐2 cells CUR affects the growth and cell viability. [ 8,9 ] Recently, Rangel‐Castañeda and coworkers demonstrated that 100–300 µ m of CUR has amoebicidal activity, affecting Entamoeba histolytica trophozoites, growth, and morphology, in a dose‐dependent behaviour, [ 10 ] in Giardia lamblia CUR inhibits the growth by affecting the microtubules; dramatic changes on cell morphology and loss of attachment were observed. [ 11 ]…”

Section: Introductionmentioning

confidence: 99%

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Encapsulation of curcumin into layered double hydroxides improve their anticancer and antiparasitic activity

Gutiérrez-Gutiérrez

Sánchez-Jiménez

Rangel-Castañeda³

et al. 2020

Journal of Pharmacy and Pharmacology

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Objectives Curcumin (CUR) has well-known activity against cancer cells and parasites; however, its applications are limited since this is an unstable molecule, which may suffer degradation by light and temperature, also, the low water solubility reduce its bioavailability. Layered double hydroxides (LDH) are well-known materials owing to the excellent anion exchange capacity, good biocompatibility and low toxicity. Methods Layered double hydroxides nanoparticles prepared with zinc and magnesium cations were used as a vehicle for CUR in Caco-2, Giardia lamblia and Entamoeba histolytica cultures. The physicochemical properties of Mg-LDH-CUR and Zn-LDH-CUR were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectrometry (FTIR) and X-ray powder diffraction (XRD). Additionally, the load efficiency, release profiles and photostability of CUR were quantified by high-performance liquid chromatography (HPLC) and UV-Vis spectrometry. Then, Mg-LDH-CUR and Zn-LDH-CUR were tested on Caco-2, G. lamblia and E. histolytica cultures. Key findings The experiments demonstrated that Zn-LDH-CUR protects better against photodegradation by UV light, while Mg-LDH-CUR showed increased toxicity against Caco-2 cell, G. lamblia and E. histolytica, in comparison with free CUR.Conclusions Layered double hydroxides are good vehicles to improve stability, resistance to degradation of CUR, also they are useful to improve solubility, provide a controlled release and improve the cytotoxic activity. Additionally, it was shown that the composition of the M +2 cation of LDH affects its properties and structure and that this directly influences its biological activity. The findings are important to select the composition of the encapsulation vehicle for a specific activity. Research PaperEncapsulation of curcumin in LDH as antiparasitic Filiberto Guti errez-Guti errez et al.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Encapsulation of curcumin into layered double hydroxides improve their anticancer and antiparasitic activity

Gutiérrez-Gutiérrez

Sánchez-Jiménez

Rangel-Castañeda³

et al. 2020

Journal of Pharmacy and Pharmacology

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“…Likewise, curcumin has been shown to possess antiparasitic activity against malaria and Giardia lamblia [32, 33]. Recently, it was determined by trypan blue exclusion test that curcumin affects the growth and cell viability of E. histolytica , in a time- and dose-dependent manner [34]. In the present work, we studied the antiamoebic activity of curcumin in the ALA model in hamster, and our results suggest that curcumin could help to greatly decrease the incidence of this type of infection.…”

Section: Discussionmentioning

confidence: 99%

Curcumin Provides Hepatoprotection against Amoebic Liver Abscess Induced by Entamoeba histolytica in Hamster: Involvement of Nrf2/HO-1 and NF-κB/IL-1β Signaling Pathways

Macías‐Pérez

Aldaba‐Muruato

Martínez-Hernández

et al. 2019

Journal of Immunology Research

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Amoebic liver abscess (ALA) is the most common extraintestinal amoebiasis caused by Entamoeba histolytica (E. histolytica). However, despite current knowledge and scientific advances about this infection, there are no effective treatments to prevent it. Herein, the antiamoebic capacity of curcumin in a hamster model was evaluated. Curcumin (150 mg/kg, p.o., daily during 10 days before infection) considerably prevents liver damage induced at 12 and 48 h post-intrahepatic inoculation of trophozoites and decreases ALT, ALP, and γ-GTP activities, and macroscopic and microscopic observations were consistent with these results. On the other hand, after one week of intraportal inoculation, liver damage was prevented by curcumin (150 mg/kg, p.o., daily, 20 days before amoebic inoculation and during the week of infection); liver/body weight ratios and tissue and histological stains showed normal appearance; in addition, the increases in ALT, ALP, and γ-GTP activities were prevented; the depletion of glycogen content induced by the amoebic damage was partially but significantly prevented, while NF-κB activity was inhibited and the expression of IL-1β was reduced; Nrf2 production showed a tendency to increase it, and HO-1 protein was overexpressed. These results suggest for the first time that curcumin can be a compound with antiamoebic effect in the liver, suggesting that its daily use could help greatly decrease the incidence of this type of infection.

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“…Dye exclusion assay was used to determine viability of trophozoites as previously described. 43,44 Briefly, after incubating neutrophils with E. histolytica trophozoites for 3 h as described in the NET formation assay, cells were collected in a 1.5 ml Eppendorf tube and centrifuged at 5000 rpm in a microcentrifuge. Cell pellet was resuspended in 40 l PBS and then 10 l of 0.4% trypan blue (Gibco R ) was added.…”

Section: Cell Viability Assaymentioning

confidence: 99%

Pathogenic Entamoeba histolytica, but not Entamoeba dispar, induce neutrophil extracellular trap (NET) formation

Fonseca

Uribe‐Querol

Díaz-Godínez

et al. 2019

Journal of Leukocyte Biology

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Amoebiasis is an infection of global importance, caused by the eukaryotic parasite Entamoeba histolytica. Pathogenic E. histolytica is associated worldwide with over a million cases of amoebic dysentery, colitis, and amoebic liver abscess. In contrast, the nonpathogenic Entamoeba dispar does not cause these diseases, although it is commonly found in the same areas as pathogenic amoeba. Entamoeba histolytica infection is usually associated with infiltrating neutrophils. These neutrophils appear to play a defensive role against this parasite, by mechanisms not completely understood. Recently, our group reported that neutrophil extracellular traps (NET) are produced in response to E. histolytica trophozoites. But, there is no information on whether nonpathogenic E. dispar can also induce NET formation. In this report, we explored the possibility that E. dispar leads to NET formation. Neutrophils were stimulated by E. histolytica trophozoites or by E. dispar trophozoites, and NET formation was assessed by video microscopy. NET induced by E. histolytica were important for trapping and killing amoebas. In contrast, E. dispar did not induce NET formation in any condition. Also E. dispar did not induce neutrophil degranulation or reactive oxygen species production. In addition, E. histolytica‐induced NET formation required alive amoebas and it was inhibited by galactose, N‐acetylgalactosamine, and lactose. These data show that only alive pathogenic E. histolytica activates neutrophils to produce NET, and suggest that recognition of the parasite involves a carbohydrate with an axial HO‐ group at carbon 4 of a hexose.

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Amoebicidal activity of curcumin on Entamoeba histolytica trophozoites

Abstract: This is the first report evaluating the effectiveness of curcumin against E. histolytica. Our results suggest that CUR could be considered for evaluation in future pharmacological studies as a promising amoebicidal agent or as complementary therapy.

Cited by 13 publications

References 30 publications

Encapsulation of curcumin into layered double hydroxides improve their anticancer and antiparasitic activity

Encapsulation of curcumin into layered double hydroxides improve their anticancer and antiparasitic activity

Curcumin Provides Hepatoprotection against Amoebic Liver Abscess Induced by Entamoeba histolytica in Hamster: Involvement of Nrf2/HO-1 and NF-κB/IL-1β Signaling Pathways

Pathogenic Entamoeba histolytica, but not Entamoeba dispar, induce neutrophil extracellular trap (NET) formation

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