Amniotic membrane transplantation for the reconstruction of the ocular surface in three cases

Abstract

This paper describes the use of amniotic membrane in the reconstructive surgical repair of generalized keratomalacia, ankyloblepharon, and after fibrous histiocytoma removal in two dogs and a cat. Case 1 was an 11-year-old female Yorkshire terrier with severe bullous keratomalacia in the left eye (OS). A frozen canine amniotic membrane graft and a third eyelid flap were applied. At day 80 postoperatively, only a mild scar and corneal vascularization were present. Case 2 was a 4-year-old female Siamese cat with symblepharon of both eyes after rhinotracheitis. Resection of the conjunctiva and frozen canine amniotic membrane transplant were performed. One month later, there was a little corneal scarring and corneal vascularization. Case 3 was a 6-year-old female terrier with a scleral and corneal mass at the 11-12 o'clock position (OS). Resection of the mass and amniotic membrane transplantation were performed. The mass was a fibrous histiocytoma localized to the sclera and cornea. This eye healed with mild conjunctivalization and no pigmentation. Amniotic membrane transplantation can be used as a method of reconstruction of the ocular surface with good repair of the cornea and minimal scarring in small animals.

Ceratoplastia lamelar em cães usando membrana amniótica equina. Estudo clínico e morfológico

“…A região de reparação apresentou maior número de camadas celulares sem, contudo, restabelecer sua arquitetura normal, sendo o mesmo processo observado por Gris et al (2002) e Godoy (2005. A formação de células epiteliais entre o estroma da córnea e a membrana amniótica, que ocorreu em um dos animais neste experimento, foi relatada anteriormente por Souza (2003); Teixeira, Barros e Martins (2004) e Godoy (2005. Esse fenômeno parece estar associado ao infiltrado de células epiteliais, sob a membrana, no momento da sutura.…”

“…O transplante pode, ainda, promover a proliferação e a diferenciação de células germinativas do limbo e da conjuntiva (MELLER et al, 2000). A membrana fetal é formada por córion, alantoide e amnion, sendo a MA a parte mais interna em contato com o feto (BARROS et al, 2005).…”

Effects of topical 0.2% Cyclosporine A on corneal neovascularization induced by xenologous amniotic membrane implantation into a corneal stroma micropocket of rats

“…In veterinary medicine its utility has been growing. For instance, it has been already used to repair full-thickness defects of the cornea of dogs (Barros et al 1998), generalized keratomalacia, ankyloblepharon, and after fibrous histiocytoma removal in two dogs and a cat (Barros et al 2005), to reconstruct corneal surface after excision of corneolimbal squamous cell carcinomas in nine horses (Olliver et al 2006), and to repair corneal ulceration and keratomalacia in three horses (Lassaline et al 2005).…”

Implante de membrana amniótica en la corrección de úlceras corneales profundas de caninos y felinos

“…Según estos estudios, la membrana amniótica provoca una rápida cicatrización del epitelio y la disminución de la estimulación nerviosa sensitiva, de forma como se evidencian en medicina humana (12,27,62,63). Una rápida epitelización se produce debido a que la membrana basal y la matriz estromal avascular actúan como el nuevo sustrato, lo que permite la reparación de queratomalasias graves en perros (62).…”