Amniotic Membrane Transplantation for the Treatment of Corneal Ulceration in Infectious Keratitis

Heiligenhaus, Arnd; Heinz, Carsten; Schmitz, Klaus; Tappeiner, Christoph; Bauer, Dirk; Meller, Daniel

doi:10.1007/978-3-540-33681-5_2

Cited by 5 publications

(34 citation statements)

References 84 publications

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“…It has structural integrity, transparency and elasticity and is known to promote epithelial cell migration, adhesion and differentiation. It has in common with basement membranes of normal cornea and conjunctiva, types IV, V and VII collagen, fibronectin and laminin 1 and 5 which facilitate corneal epithelial cell adhesion and anchorage to stroma 1,7,22 . It is a superior substrate for the growth and proliferation of epithelial cells and their progenitors.…”

Section: Scientific Basis Of Clinical Applicationmentioning

confidence: 99%

“…This previously described anti‐inflammatory action is one way in which amnion decreases corneal neovascularization. Another is via the release of soluble anti‐angiogenic factors from the epithelial and mesenchymal cells of amniotic membrane, including interleukin‐1 receptor antagonist, all four types of tissue inhibitors of metalloproteinase (TIMP), collagen 18, IL‐10, thrombospondin‐1, and pigment epithelium‐derived factor (PEDF) 7,26 . These factors decrease vascular endothelial cell proliferation.…”

Section: Scientific Basis Of Clinical Applicationmentioning

confidence: 99%

“…There is evidence that amniotic membrane has diverse properties against bacterial and viral infections 7 . The fact that the chorioamnion prevents the spread of bacteria from the maternal to the fetal suggests that it may function as a physical barrier against infection.…”

Section: Scientific Basis Of Clinical Applicationmentioning

confidence: 99%

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The use of amniotic membrane transplantation for ocular surface reconstruction: a review and series of 58 equine clinical cases (2002–2008)

Plummer

2009

Veterinary Ophthalmology

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Amniotic membrane transplantation (AMT) is an effective clinical therapy for reconstruction of the ocular surface in human and veterinary patients. Amnion is avascular and strong, contains antiangiogenic and antiinflammatory properties and growth factors, and has properties that prevent or decrease fibrosis in healing tissue. Indications for its use are steadily growing and include grafting to replace diseased, missing or excised tissue, patching to support diseased tissue during the healing process and as a substrate for the expansion of epithelial cells for transplantation to the cornea. AMT through a combination of mechanical and biologic factors can preserve the integrity of the globe, optimize the visual outcome, and minimize scarring in severely diseased corneas.

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Section: Scientific Basis Of Clinical Applicationmentioning

confidence: 99%

Section: Scientific Basis Of Clinical Applicationmentioning

confidence: 99%

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The use of amniotic membrane transplantation for ocular surface reconstruction: a review and series of 58 equine clinical cases (2002–2008)

Plummer

2009

Veterinary Ophthalmology

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“…Imediatamente após a cauterização, 0,05 ml (1,25 mg) factors responsible for the inducement of inflammation include vascular endothelial growth factor (VEGF), transforming growth factors, platelet-activating factor, basic fibroblast growth factor, and tumor necrosis factor-α (5)(6)(7) . It has previously been reported that VEGF is upregulated in corneas with inflammation and vascularisation and that it is a significant angiogenic factor in corneal neovascularization (8,9) .…”

Section: Métodos: Córneas Direitas De 24 Ratos Wistar-albino Foram Camentioning

confidence: 99%

Comparison of the effects of subconjunctival and topical anti-VEGF therapy (bevacizumab) on experimental corneal neovascularization

Özdemir¹,

Altıntaş²,

Altıntaş³

et al. 2014

Arquivos Brasileiros De Oftalmologia

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Purpose: To compare the effects of bevacizumab applied either subconjunctivally or topically, in a rat model of corneal neovascularization induced by alkali burn. Methods: The right corneas of 24 Wistar-Albino rats were cauterized with silver nitrate sticks. The rats were divided randomly and equally into three groups: no treatment control (n=8), subconjunctival bevacizumab treatment (n=8), and topical bevacizumab treatment (n=8). Immediately following cauterization, the subconjunctival group was treated with a 0.05 ml (1.25 mg) bevacizumab subconjunctival injection. The topical group was treated with 10 mg/ml bevacizumab twice daily, and the control group received subconjunctival saline injections twice daily. The burn stimulus and neovascularization scores were evaluated using a technique previously described by Mahoney and Waterbury. Digital photographs were obtained before the eyes were enucleated and corneal sections were then analyzed by histopathology. Results: The mean burn stimulus score was 1.86 ± 0.6 and there was no statistical difference between the groups (p=0.730). The mean neovascularization scores in the subconjunctival and topical bevacizumab groups were statistically lower than the control group (p<0.05). The mean percentage area of corneal neovascularization was 82.5 ± 22.1 in the control group, 42.7 ± 15.0 in the subconjunctival group, and 55.8 ± 18.2 in the topical group. The differences between the control and treatment groups were statistically significant (p<0.05). Histopathology showed that the treatment groups presented less neovascularization, inflammation, and fibroblast activity than the control group (p<0.05). Conclusions: This study demonstrates that both subconjunctival and topical administrations of bevacizumab inhibit corneal neovascularization and decrease inflammation and fibroblast activity in a rat model of corneal neovascularization induced by alkali burn.

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“…It has been accepted as an antiangiogenic pharmaceutical for the treatment of certain types of cancers and it has also been used to treat ocular neovascularization [10]. In numerous studies it has been reported that topical or subconjunctival Bevacizumab application results in the inhibition or regression of corneal neovascularization [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Therapeutic Effects of Extracts from Spirulina platensis versus Bevacizumab on Inflammation-Associated Corneal Neovascularization

El-Shazly¹,

Ahmed²,

Elzankalony³

et al. 2017

J Med Surg Pathol

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Aim: The aim of this work was to study histological and immunohistochemical changes resulting from corneal alkali burn and to assess these changes after treatment with either polysaccharide extract from Spirulina platensis (PSP) or Bevacizumab. Methods:Alkali burn was induced in corneas by direct application of a filter paper ring (2.5 mm diameter) saturated with 1 N NaOH and applied to the center of the rat corneas for 30 seconds. PSP (extracted from dry powder of Spirulina platensis) and avastin were administered topically on the corneas 4 times daily for 7 days starting 7 days after induction of corneal alkali burn. The therapeutic effects of PSP and avastin were evaluated daily using slit-lamp. At the end of the therapy, corneas were harvested for H and E staining, Masson trichrome staining, immunohistochemical study for assessment of inflammation and corneal neovascularization (CorNV).Results: Topical application of either PSP extract or Avastin had significant therapeutic effects on corneal injuries induced by alkali burn. This was demonstrated by decreasing the CorNV, total and differential corneal thickness and inflammatory cellular responses. They increased the epithelial thickness. PSP showed better results than Avastin in this regards. Conclusion:The naturally occurring Spirulina platensis (PSP) is more cost effective in treatment of corneal neovascularization and decrease inflammation and fibroblast activity in a rat model of corneal neovascularization induced by alkali burn as compared to Avastin.

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Amniotic Membrane Transplantation for the Treatment of Corneal Ulceration in Infectious Keratitis

Cited by 5 publications

References 84 publications

The use of amniotic membrane transplantation for ocular surface reconstruction: a review and series of 58 equine clinical cases (2002–2008)

The use of amniotic membrane transplantation for ocular surface reconstruction: a review and series of 58 equine clinical cases (2002–2008)

Comparison of the effects of subconjunctival and topical anti-VEGF therapy (bevacizumab) on experimental corneal neovascularization

Therapeutic Effects of Extracts from Spirulina platensis versus Bevacizumab on Inflammation-Associated Corneal Neovascularization

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