Amniotic Membrane Transplantation for Persistent Epithelial Defects With Ulceration

Lee, Shwu‐Huey; Tseng, Scheffer C.G.

doi:10.1016/s0002-9394(14)70125-4

Cited by 606 publications

(372 citation statements)

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“…It has been found useful in the treatment of persistent epithelial defect (PED) with ulceration, 16 primary, and recurrent pterygium with symblepharon, 17 for conjunctival surface reconstruction 18 and in combination with limbal grafts in the reconstruction of ocular surface in advanced ocular cicatricial pemphigoid and Steven-Johnson syndrome 19 as well as chemical and thermal burns. 20 Reports regarding the role of AMT in acute ocular burns success are highly variable.…”

Section: Discussionmentioning

confidence: 99%

Amniotic membrane transplantation in acute chemical burns

Arora

Mehta

Jain

2004

Eye

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Section: Discussionmentioning

confidence: 99%

Amniotic membrane transplantation in acute chemical burns

Arora

Mehta

Jain

2004

Eye

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“…Briefly, human placenta was collected at elective cesarean delivery (Heiligenhaus et al, 2001;Lee and Tseng, 1997;Prabhasawat et al, 1997;Tseng et al, 1998). The amniotic membrane was flattened onto nitrocellulose paper (Hybond N+, Amersham, UK), with the epithelium surface up.…”

Section: Preparation Of Preserved Human Amniotic Membranementioning

confidence: 99%

On the influence of neutrophils in corneas with necrotizing HSV-1 keratitis following amniotic membrane transplantation

Bauer

Wasmuth

Hermans

et al. 2007

Experimental Eye Research

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Necrotizing herpetic stromal keratitis (HSK) in mice rapidly improved after amniotic membrane transplantation (AMT). In this study we determined the fate of polymorphonuclear neutrophils (PMN) after AMT. AMT or tarsorrhaphy (T) was performed in BALB/c mice with ulcerative HSK. After 2 days, corneas were studied histologically and by transmission electron microscopy (TEM). CD11b, Gr-1, and TUNEL-positive cells were identified. Macrophages were depleted by subconjunctival injection of dichloromethylene-diphosphonate-liposomes (Cl 2 MDP-LIP) before AMT. Corneas were studied for interleukin (IL)-1α, IL-2, interferon (IFN)-γ, CXCL1, CXCL2, and tumor necrosis factor (TNF)-α production by ELISA. PMN-enriched cell preparations cocultured with amniotic membrane (AM) or with AM and such recombinant (r) cytokines as rIL-1α, rIL-2, and rTNF-α or supernatants from activated lymphocytes were investigated by flow cytometry (Annexin-V/7-AAD and TUNEL), and a dimethylthiazolyl-diphenyltetrazoliumbromide (MTT)-viability assay. Corneas in the AMT mice had less inflammation, fewer PMN-like cells and fewer CD11b+, and Gr-1+ cells (P < 0.01), but a higher ratio of apoptotic to viable PMN-resembling cells (P < 0.01) than the T mice. Phagocytic removal of apoptotic PMN-like cells by macrophages was evident in the AMT group. After Cl 2 MDP-LIP treatment, the corneas had more cell debris and apoptotic cells with PMN-like morphology. The concentrations of IL-1 α, IL-2, CXCL1, and TNF-α were reduced in corneas of the AMT group as compared to that of the T group, while the concentration of CXCL2 was increased. Apoptosis of PMN-resembling cells was detected following cocultivation with AM, even when proinflammatory cytokines were present. Resolution of corneal inflammation in mice with necrotizing HSK after AMT is associated with increased apoptosis of PMN-like cells, reduction of pro-inflammatory cytokines, an increase of CXCL2, and increased removal of apoptotic PMN-like cells by macrophages.

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“…The disorder was presumed to involve primarily the RPE, 1 whereas other investigators proposed that the underlying process in APMPPE is an inflammatory vasculitis affecting the choroidal vessels and that the RPE was secondarily affected. [2][3][4][5][6] This report describes a patient in whom the clinical and fluorescein angiography findings were typical of APMPPE but in whom retinal vasculitis and papillitis were additional features.…”

Section: Commentsmentioning

confidence: 89%

“…Human amniotic membrane was prepared and preserved by the standard method. 4 The human placenta was obtained shortly after elective caesarian delivery. Serological tests were performed to exclude human immunodeficiency virus, hepatitis virus type B, hepatitis virus type C and syphilis.…”

Section: Casementioning

confidence: 99%