Amniotic Membrane Transplantation for Conjunctival Surface Reconstruction

Tseng, Scheffer C.G.; Prabhasawat, Pinnita; Lee, Shwu‐Huey

doi:10.1016/s0002-9394(14)71693-9

Cited by 386 publications

(255 citation statements)

References 22 publications

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“…It has been found useful in the treatment of persistent epithelial defect (PED) with ulceration, 16 primary, and recurrent pterygium with symblepharon, 17 for conjunctival surface reconstruction 18 and in combination with limbal grafts in the reconstruction of ocular surface in advanced ocular cicatricial pemphigoid and Steven-Johnson syndrome 19 as well as chemical and thermal burns. 20 Reports regarding the role of AMT in acute ocular burns success are highly variable.…”

Section: Discussionmentioning

confidence: 99%

Amniotic membrane transplantation in acute chemical burns

Arora

Mehta

Jain

2004

Eye

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Section: Discussionmentioning

confidence: 99%

Amniotic membrane transplantation in acute chemical burns

Arora

Mehta

Jain

2004

Eye

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“…Amniotic membrane transplantation (AMT) used as a "graft" has been advocated as a basement membrane substitute (Shimazaki et al, 1998;Tseng et al, 1997). It has been reported that even deep corneal ulcers can be successfully treated with AMT (Hanada et al, 2001;Kruse et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

On the influence of neutrophils in corneas with necrotizing HSV-1 keratitis following amniotic membrane transplantation

Bauer

Wasmuth

Hermans

et al. 2007

Experimental Eye Research

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Necrotizing herpetic stromal keratitis (HSK) in mice rapidly improved after amniotic membrane transplantation (AMT). In this study we determined the fate of polymorphonuclear neutrophils (PMN) after AMT. AMT or tarsorrhaphy (T) was performed in BALB/c mice with ulcerative HSK. After 2 days, corneas were studied histologically and by transmission electron microscopy (TEM). CD11b, Gr-1, and TUNEL-positive cells were identified. Macrophages were depleted by subconjunctival injection of dichloromethylene-diphosphonate-liposomes (Cl 2 MDP-LIP) before AMT. Corneas were studied for interleukin (IL)-1α, IL-2, interferon (IFN)-γ, CXCL1, CXCL2, and tumor necrosis factor (TNF)-α production by ELISA. PMN-enriched cell preparations cocultured with amniotic membrane (AM) or with AM and such recombinant (r) cytokines as rIL-1α, rIL-2, and rTNF-α or supernatants from activated lymphocytes were investigated by flow cytometry (Annexin-V/7-AAD and TUNEL), and a dimethylthiazolyl-diphenyltetrazoliumbromide (MTT)-viability assay. Corneas in the AMT mice had less inflammation, fewer PMN-like cells and fewer CD11b+, and Gr-1+ cells (P < 0.01), but a higher ratio of apoptotic to viable PMN-resembling cells (P < 0.01) than the T mice. Phagocytic removal of apoptotic PMN-like cells by macrophages was evident in the AMT group. After Cl 2 MDP-LIP treatment, the corneas had more cell debris and apoptotic cells with PMN-like morphology. The concentrations of IL-1 α, IL-2, CXCL1, and TNF-α were reduced in corneas of the AMT group as compared to that of the T group, while the concentration of CXCL2 was increased. Apoptosis of PMN-resembling cells was detected following cocultivation with AM, even when proinflammatory cytokines were present. Resolution of corneal inflammation in mice with necrotizing HSK after AMT is associated with increased apoptosis of PMN-like cells, reduction of pro-inflammatory cytokines, an increase of CXCL2, and increased removal of apoptotic PMN-like cells by macrophages.

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“…The use of AMT for conjunctival surface reconstruction has been described recently, first in rabbits (Kim & Tseng 1995) and then in humans (Tseng et al 1997). Our clinical result shows that AMT can bring about pain reduction, symblepharon and entropion resolution, and end the continual damage to the ocular surface epithelia that results from recurrent erosions, ulcer formation and secondary bacterial infections.…”

Section: Discussionmentioning

confidence: 99%

Amniotic membrane transplantation elicits goblet cell repopulation after conjunctival reconstruction in a case of severe ocular cicatricial pemphigoid

Barabino

Rolando

2003

Acta Ophthalmologica Scandinavica

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ABSTRACT.Purpose: The surgical procedures used until now to rebuild the conjunctiva in patients affected by ocular cicatricial pemphigoid (OCP) have not demonstrated appreciable anatomical and functional success. This study reports the postoperative clinical and cytological outcomes of a human amniotic membrane transplantation (AMT) to rebuild the conjunctiva in a case of late stage OCP. Methods: We present a 75-year-old woman with a severe form of pemphigoid with entropion, trichiasis, symblepharon and blunting of the fornices, who underwent excision of the scarred and inflamed tissue covering the ocular surface and AMT. Results: Our data show an improvement of the ocular surface condition, with reacquired fornix depth, reduced inflammation and presence of goblet cells at each follow-up. Conclusion: Amniotic membrane transplantation was successful as a first step procedure to reduce inflammation and to rebuild a physiological conjunctival epithelium in late stage OCP.

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Amniotic Membrane Transplantation for Conjunctival Surface Reconstruction

Cited by 386 publications

References 22 publications

Amniotic membrane transplantation in acute chemical burns

Amniotic membrane transplantation in acute chemical burns

On the influence of neutrophils in corneas with necrotizing HSV-1 keratitis following amniotic membrane transplantation

Amniotic membrane transplantation elicits goblet cell repopulation after conjunctival reconstruction in a case of severe ocular cicatricial pemphigoid

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