Amniotic membrane as novel scaffold for human iPSC-derived cardiomyogenesis

Parveen, Shagufta; Singh, Shishu Pal; Panicker, Mitradas M.; Gupta, Pawan Kumar

doi:10.1007/s11626-019-00321-y

Cited by 16 publications

(10 citation statements)

References 55 publications

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“…As such, increased cTE construct complexity, e.g., constructs composed of multiple cell types, densely organized into a native tissue‐like architecture, will benefit from a tailored preservation approach. Currently, three classes of cTE constructs can be distinguished: thin (<300 µm) composed of a cell sheet or monolayer [ 112 ] ; thick (>300 µm) achieved by stacking multiple cell sheets or use of scaffolds; and advanced with higher complexity (>300 µm) where the diffusion of molecules is increased by vascularization [ 113–115 ] or forced flow of medium. [ 116 ] Those exhibiting a close‐to‐native thickness (i.e., 5.3 to 9 mm) [ 117 ] may hold the greatest clinical potential, as these are expected to provide the strongest contractile support, although the force produced by a construct does not merely depend on its size, but also on cell density, maturity, and organization.…”

Section: A Roadmap To Cte Construct Preservationmentioning

confidence: 99%

A Roadmap to Cardiac Tissue‐Engineered Construct Preservation: Insights from Cells, Tissues, and Organs

et al. 2021

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Worldwide, over 26 million patients suffer from heart failure (HF). One strategy aspiring to prevent or even to reverse HF is based on the transplantation of cardiac tissue‐engineered (cTE) constructs. These patient‐specific constructs aim to closely resemble the native myocardium and, upon implantation on the diseased tissue, support and restore cardiac function, thereby preventing the development of HF. However, cTE constructs off‐the‐shelf availability in the clinical arena critically depends on the development of efficient preservation methodologies. Short‐ and long‐term preservation of cTE constructs would enable transportation and direct availability. Herein, currently available methods, from normothermic‐ to hypothermic‐ to cryopreservation, for the preservation of cardiomyocytes, whole‐heart, and regenerative materials are reviewed. A theoretical foundation and recommendations for future research on developing cTE construct specific preservation methods are provided. Current research suggests that vitrification can be a promising procedure to ensure long‐term cryopreservation of cTE constructs, despite the need of high doses of cytotoxic cryoprotective agents. Instead, short‐term cTE construct preservation can be achieved at normothermic or hypothermic temperatures by administration of protective additives. With further tuning of these promising methods, it is anticipated that cTE construct therapy can be brought one step closer to the patient.

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Section: A Roadmap To Cte Construct Preservationmentioning

confidence: 99%

A Roadmap to Cardiac Tissue‐Engineered Construct Preservation: Insights from Cells, Tissues, and Organs

et al. 2021

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“…Better interaction and growth of differentiated ESCs were observed on the PLGA/Col scaffolds relative to PLGA-only scaffolds. On the other hand, a cryopreserved amniotic membrane has been shown to direct the differentiation of hiPSC-derived cardiac progenitor cells to CMs in the presence of cytokines [41]. Amniotic membranes increased the expression of cardiac transcription factors and myofibril proteins, accelerated the intracellular calcium transients, and enhanced the mitochondrial complexity formation in CMs.…”

Section: Tissue Engineering For the Differentiation Of Pscs Into Cardiovascular Cellsmentioning

confidence: 99%

“…The low engraftment rate can be improved by combining the technique with cardiac tissue engineering, in which cells are transplanted within a supporting matrix [8]. Biomaterials can provide mechanical support for the stem cells and supply nutrients and oxygen to encapsulated cells [9,10,41,42].…”

Section: Tissue Engineering Using Psc-derived Cardiovascular Cells For the Treatment Of MImentioning

confidence: 99%

Cardiac Tissue Engineering for the Treatment of Myocardial Infarction

Wang

2021

JCDD

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Poor cell engraftment rate is one of the primary factors limiting the effectiveness of cell transfer therapy for cardiac repair. Recent studies have shown that the combination of cell-based therapy and tissue engineering technology can improve stem cell engraftment and promote the therapeutic effects of the treatment for myocardial infarction. This mini-review summarizes the recent progress in cardiac tissue engineering of cardiovascular cells from differentiated human pluripotent stem cells (PSCs), highlights their therapeutic applications for the treatment of myocardial infarction, and discusses the present challenges of cardiac tissue engineering and possible future directions from a clinical perspective.

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“…13 Various cells, including cardiomyocytes, mesenchymal stem cells, broblasts, and limbal stromal cells, have been successfully cultured on placental membrane scaffolds. [14][15][16][17] These exceptional ACM properties can be attributed to the components of its extracellular matrix (ECM) and basement membranes. The basement membrane under the epithelial layer of amnion and the trophoblastic layer of chorion contains collagen type III, type IV and type V, laminin, and bronectin which can act as a suitable bed for vascular growth.…”

Section: Introductionmentioning

confidence: 99%

Developing a Pro-Angiogenic Amniochorionic-Derived Decellularized Scaffold with Two Exposed Basement Membranes for Cultivating Endothelial Cells

Shariatzadeh

Shafiee

Tayebi

et al. 2021

Preprint

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Decellularized placental membrane has widely been used as scaffold and graft in tissue engineering and regenerative medicine. Exceptional pro-angiogenic and biomechanical properties and low immunogenicity have made the amniochorionic membrane a unique scaffold which provides enriched niche for cellular growth. Herein, an optimized combination of enzymatic solutions (based on Streptokinase) with mechanical scrapping is used to remove the amniotic epithelium and chorion trophoblastic layer, which results in exposing the basement membranes of both sides without their separation and subsequent damages to the in-between spongy layer. Biomechanical and biodegradability properties, endothelial proliferation capacity, and in-vivo pro-angiogenic capabilities of the scaffold were also evaluated. Histological staining and scanning electron microscope (SEM) demonstrated that the underlying amniotic and chorionic basement membranes remained intact while the epithelial and trophoblastic layers were entirely removed without considerable damage to basement membranes. The biomechanical evaluation showed that the scaffold is suturable. Proliferation assay and immunohistochemistry demonstrated that both side basement membranes could support growth of endothelial cells without altering endothelial characteristics. The dorsal skinfold chamber animal model indicated that both side basement membranes could promote angiogenesis. This bi-sided decellularized scaffold with two exposed surfaces for cultivating various cells would have potential applications in skin, cardiac, vascularized composite allografts, and microvascular tissue engineering.

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Amniotic membrane as novel scaffold for human iPSC-derived cardiomyogenesis

Cited by 16 publications

References 55 publications

A Roadmap to Cardiac Tissue‐Engineered Construct Preservation: Insights from Cells, Tissues, and Organs

A Roadmap to Cardiac Tissue‐Engineered Construct Preservation: Insights from Cells, Tissues, and Organs

Cardiac Tissue Engineering for the Treatment of Myocardial Infarction

Developing a Pro-Angiogenic Amniochorionic-Derived Decellularized Scaffold with Two Exposed Basement Membranes for Cultivating Endothelial Cells

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