Amniotic membrane as a potent source of stem cells and a matrix for engineering heart tissue

Francisco, Júlio César; Cunha, Ricardo; Simeoni, Rossana Baggio; Guarita-Souza, Luiz César; Ferreira, Reginaldo Justino; Irioda, Ana Carolina; Souza, Carolina Maria Costa Oliveira de; Gadicherla, Srikanth; Nityanand, Soniya; Chachques, Juan Carlos; Carvalho, Katherine Athayde Teixeira de

doi:10.4236/jbise.2013.612147

Cited by 14 publications

(8 citation statements)

References 55 publications

(94 reference statements)

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“…epithelial cells are removed from the AM and recellularized with tenocytes to prepare a tissue-engineered tendon. When AM is decellularized, it is left with the matrix that rich in ECM proteins such as collagen from various type including type I and type IV, fibronectin, laminin, and ground substances such as proteoglycans, that provide important cues for cellular proliferation, growth, signaling and communication [84]. Decellularization AM has been reported to promote cell adhesion, proliferation, differentiation and stratification as well as more uniform cell growth compared to intact AM [85].…”

Section: Human Amniotic Membrane As a Scaffold For Tendon/ligament Timentioning

confidence: 99%

Current Progress in Tendon and Ligament Tissue Engineering

Lim

Liau

et al. 2019

Tissue Eng Regen Med

154

146

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BACKGROUND: Tendon and ligament injuries accounted for 30% of all musculoskeletal consultations with 4 million new incidences worldwide each year and thus imposed a significant burden to the society and the economy. Damaged tendon and ligament can severely affect the normal body movement and might lead to many complications if not treated promptly and adequately. Current conventional treatment through surgical repair and tissue graft are ineffective with a high rate of recurrence. METHODS: In this review, we first discussed the anatomy, physiology and pathophysiology of tendon and ligament injuries and its current treatment. Secondly, we explored the current role of tendon and ligament tissue engineering, describing its recent advances. After that, we also described stem cell and cell secreted product approaches in tendon and ligament injuries. Lastly, we examined the role of the bioreactor and mechanical loading in in vitro maturation of engineered tendon and ligament. RESULTS: Tissue engineering offers various alternative ways of treatment from biological tissue constructs to stem cell therapy and cell secreted products. Bioreactor with mechanical stimulation is instrumental in preparing mature engineered tendon and ligament substitutes in vitro. CONCLUSIONS: Tissue engineering showed great promise in replacing the damaged tendon and ligament. However, more study is needed to develop ideal engineered tendon and ligament.

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Section: Human Amniotic Membrane As a Scaffold For Tendon/ligament Timentioning

confidence: 99%

Current Progress in Tendon and Ligament Tissue Engineering

Lim

Liau

et al. 2019

Tissue Eng Regen Med

154

146

View full text Add to dashboard Cite

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“…It is believed that the conditioned medium (CM) of stem cells might provide more functional capabilities (Ahmadi et al., 2017) owing to paracrine secretion of cytokines (Keyhanmanesh, Rahbarghazi, & Ahmadi, 2018). Previous studies have shown that human amniotic membrane (hAM) is an MSC‐rich tissue with considerable anti‐inflammatory, antifibrotic and antioxidant properties (Francisco et al., 2013; Magatti et al., 2015; Riedel et al., 2019), and its CM has similar immunomodulatory properties (Fu, Ohnishi, & Sakamoto, 2018; Magatti et al., 2015; Pan et al., 2019). In this study, we aimed to examine the effect of hAM‐MSC‐CM for treatment of a murine model of ovalbumin (OVA)‐induced subacute allergic asthma.…”

Section: Introductionmentioning

confidence: 99%

Human amniotic membrane mesenchymal stem cell‐conditioned medium reduces inflammatory factors and fibrosis in ovalbumin‐induced asthma in mice

Dalouchi

Falak

Bakhshesh³

et al. 2021

Experimental Physiology

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New Findings What is the central question of this study?Is mesenchymal stem cell‐conditioned medium capable of improving the pathological alterations of ovalbumin‐induced asthma in mice? What is the main finding and its importance?Our study indicated that human amniotic membrane mesenchymal stem cell‐conditioned medium is capable of modulating inflammation, fibrosis, oxidative stress and the pathological consequences of ovalbumin‐induced allergic asthma in mice. Abstract Paracrine factors secreted by mesenchymal stem cells (MSCs) have immunomodulatory, anti‐inflammatory and antifibrotic properties, and the conditioned medium (CM) of these cells might have functional capabilities. We examined the effects of human amniotic membrane MSC‐CM (hAM‐MSC‐CM) on ovalbumin (OVA)‐induced asthma. Forty male Balb/c mice were randomly divided into the following four groups: control; OVA (sensitized and challenged with OVA); OVA+CM (sensitized and challenged with OVA and treated with hAM‐MSC‐CM); and OVA+Placebo (sensitized and challenged with OVA and treated with placebo). Forty‐eight hours after the last challenge, serum and bronchoalveolar lavage fluid samples were collected and used for evaluation of inflammatory factors and cells, respectively. Lung tissue sections were stained with Haematoxylin and Eosin or Masson's Trichrome to evaluate pathological changes, and oxidative stress was assessed in fresh lung tissues. Treatment with hAM‐MSC‐CM significantly hindered histopathological changes and fibrosis and reduced the total cell count and the percentage of eosinophils and neutrophils in bronchoalveolar lavage fluid. Furthermore, it reduced serum levels of immunoglobulin E, interleukin‐4, transforming growth factor‐β and lung malondialdehyde. It also increased serum levels of interferon‐γ and interleukin‐10, in addition to the enzymatic activity of glutathione peroxidase, catalase and superoxide dismutase in lung tissue in comparison to the OVA and OVA+Placebo groups. This study showed that administration of hAM‐MSC‐CM can improve pathological conditions, such as inflammation, fibrosis and oxidative stress, in OVA‐induced allergic asthma.

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“…Bone marrow MSCs were taken from the femur and tibia of rats and co-cultured indirectly with cardiomyocytes taken from the ventricular wall at the 1:10 ratio, separated with a semipermeable membrane, and then cultivated for 1-3 weeks. It was shown that the resulting cells expressed SERCA2 and RyR genes, and, when implanted in rats, cardiac troponin T, cardiac troponin I, α-actinin, and desmin were expressed in the sections [42].…”

Section: Figure 4 Gene Expression In the 3-d Co-cultures Of The Haesc...mentioning

confidence: 99%

Co-culture of human cardiomyocyte and human amnion epithelial stem cells in amnion bilayer matrix for cardiomyogenesis

Putra¹,

Sandora²,

Kusuma³

et al. 2022

CTT

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Our study developed a culture system to deliver cells for the regeneration of infarcted myocardial tissue. Human cardiomyocytes (hCardio) and human amnion epithelial stem cells (hAESC) were co-cultured in a biological scaffold forming a 3-D matrix, prepared as a heart patch candidate. The current investigation was aimed for assessment of hCardio-to-hAESC seeding ratio for the best conditions to provide cardiomyogenesis, i.e., 1:5 or the 1:6. This ratio corresponded to optimal cell number of 500,000 cells/cm 2 , thus providing a 12-cm 2 heart patch. This cell ratio was also published in our previous study using human adipose stem cells and hCardio. The hCardio was isolated from the heart tissue taken during surgical correction performed in patients with right ventricular hypertrophy. The cell isolates expressed cTnT (10.7%), cKit/CD117 (16%), ICAM (94%), and PECAM+/VCAM-33%. The hAESC was isolated from an elected donor at Caesarean section. It showed expression of TRA-1-60 (82.4%), SSEA-4 (28.2%), Oct-3/4 (2.9%), Nanog (11.4%), without expression of immune antigens, e.g., HLA-DR (0%), HLA-ABC (0.2%) as well as low-level expression of mesenchymal stem cell (MSC) markers, i.e., CD73 (20.2%), CD90 (0.4%), CD105 (59.2%). These cells did not exhibit Lin phenotype (CD3, CD14, CD16, CD19, CD20, CD56).At both seeding ratios, the co-culture matrix also released TNF-α at low levels (<1 pg/ml) throughout cultivation from day 1 to day 8. Both groups had shown a consistent growth of cells over time. Confocal images showed that the cell population has expanded and migrated to the deeper plane up to 140 µm after 5 days of incubation in both 1:5 and 1:6 groups. The cells seemed to connect and form projections, starting by day 5, being more obvious among the 1:5 group. They proliferated rapidly until no solitary cells were observed after day 8 of co-culture. Expression of the cardiomyogenesis genes, e.g., cTnT and ACTN2 in the 1:6 group were expressed, similarly to normal cardiomyocytes after eight days of cultivation. MHC genes in the 1:6 group were also expressed, although less than 1-fold to normal cardiomyocytes. This study indicated that hCardio and hAESC at 1:6 ratio seeded on amnion bilayer could support cardiomyogenesis derivation from the progenitor cells.

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Amniotic membrane as a potent source of stem cells and a matrix for engineering heart tissue

Cited by 14 publications

References 55 publications

Current Progress in Tendon and Ligament Tissue Engineering

Current Progress in Tendon and Ligament Tissue Engineering

Human amniotic membrane mesenchymal stem cell‐conditioned medium reduces inflammatory factors and fibrosis in ovalbumin‐induced asthma in mice

Co-culture of human cardiomyocyte and human amnion epithelial stem cells in amnion bilayer matrix for cardiomyogenesis

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