Amniotic membrane as a biological dressing for 5-fluoruracil-induced oral mucositis in rats

Lima, Gabriela de Morais Gouvêa; Severo, M.C.; Santana-Melo, Gabriela de Fátima; Carvalho, Marle Araújo Luz De; Vilela-Goulart, Maria das Graças; Salgado, Miguel Angel Castillo; Gomes, Mônica Fernandes

doi:10.1016/j.ijom.2015.01.007

Cited by 10 publications

(11 citation statements)

References 14 publications

(29 reference statements)

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“…In particular, the AM is a huge source for multipotent mesenchymal stem cells (MSCs) with the ability to differentiate into a wide variety of cells, such as chondroblasts, osteoblasts, adipocytes and fibroblasts, myocytes, endothelial cells, neuronal cells, and hepatocytes, leading to formation of cartilage, bone, connective tissue, muscle, blood vessel, nerve, and liver tissue, respectively [56][57][58]. This membrane acts as a barrier, preventing the entry of pathogens and toxins, preserving the tissue structures, and, consequently, reducing the levels of local proinflammatory cytokines [58,59]. Thus, it could be largely used to selectively guide the tissue regeneration in the periodontium following destructive PD.…”

Section: Alternative Therapy For Periodonticsmentioning

confidence: 99%

Periodontal Diseases in Patients with Special Health Care Needs

Gomes¹,

Marco²,

Giannasi³

et al. 2019

Periodontology and Dental Implantology

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A wide variation of people with an impairment or disability requires a "special care dentistry" once their general manifestations directly act in the oral cavity. This target public is inserted into the following categories: neuromotor disability, sensory disability, mental disorder, infecto-contagious diseases, chronic systemic diseases, and systemic conditions. Among the several oral illnesses found in these groups, periodontal diseases have been the most frequent, becoming a major challenge for the dental practitioners. Thus, we described the microbiological, histopathological, and clinical features of periodontal diseases in each "special health care needs" group. Advances in "Omic" technologies have suggested the application of molecular biology methods to assess the genomics (genes), proteomics (proteins), transcriptomics (mRNA), and metabolomics (metabolites) aspects of periodontal diseases. These researches aim to promote a better understanding of the mechanisms involved in the pathogenesis and in the identification of new biomarkers of periodontal diseases that help in diagnosis of periodontal diseases and in tissue responses after treatments of gingivitis and periodontitis. As an alternative therapy, some bioactive materials and photobiomodulation may be indicated once they strongly stimulate the periodontal tissue regeneration, attenuate the inflammatory processes, and/ or promote the reconstruction of the microstructure of the periodontium.

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Section: Alternative Therapy For Periodonticsmentioning

confidence: 99%

Periodontal Diseases in Patients with Special Health Care Needs

Gomes¹,

Marco²,

Giannasi³

et al. 2019

Periodontology and Dental Implantology

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“…according to Lima et al (2015). On day two, the animals were anesthetized [ketamine hydrochloride 10% (50 mg/ …”

Section: Chemotherapy-induced Oral Mucositismentioning

confidence: 99%

Anti-inflammatory and healing action of oral gel containing borneol monoterpene in chemotherapy-induced mucositis in rats ( Rattus norvegicus )

Júnior

Brito

Barros

et al. 2017

Braz. J. Pharm. Sci.

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The aim of this study was to investigate the effect of gels containing the monoterpene borneol in induced oral mucositis using an animal model. Gels were prepared with borneol at 1.2% and 2.4% (w/w). Oral mucositis was induced by administration of three doses of 5-fluorouracil (30 mg/kg, i.p.) and injury with acetic acid (50%, v/v) soaked in filter paper applied to right cheek mucosa for 60s. Four subgroups comprising 12 animals each were formed. Six animals from each group were sacrificed at days seven and fourteen after oral mucositis induction. Mucous samples were processed and stained with hematoxylineosin and Masson's Trichrome. The semiquantitative evaluation involved observation of inflammatory parameters. ImageJ® software was used in the quantitative evaluation. For statistical analyses, Two-way ANOVA, followed by Tukey's post-test (p <0.05), were employed. Borneol 2.4% gel proved effective in the treatment of oral mucositis with statistically significant differences between groups for angiogenesis control, inflammatory cell count reduction and percentage neoformed collagen increase. The confirmation of anti-inflammatory and healing action of borneol in oral mucositis in rats renders it a good marker for predicting this activity for plant extracts rich in this substance.

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“…AM corresponds to the innermost layer of placenta. It is composed of a single epithelial layer, a basement membrane and an avascular stroma with growth factors, cytokines and some bioactive substances [ 115 , 116 ]. These factors contribute to its anti-inflammatory, anti-fibrotic, anti-microbial, anti-scarring and low immunogenicity properties as well as to promote neovascularization [ 116 ].…”

Section: Introductionmentioning

confidence: 99%

“…It is composed of a single epithelial layer, a basement membrane and an avascular stroma with growth factors, cytokines and some bioactive substances [ 115 , 116 ]. These factors contribute to its anti-inflammatory, anti-fibrotic, anti-microbial, anti-scarring and low immunogenicity properties as well as to promote neovascularization [ 116 ]. Lima and collaborators (2015) used rat models of 5-FU-induced OM to test AM or not, as a natural dressing biomaterial, during 14 or 21 days [ 116 ].…”

Section: Introductionmentioning

confidence: 99%

“…These factors contribute to its anti-inflammatory, anti-fibrotic, anti-microbial, anti-scarring and low immunogenicity properties as well as to promote neovascularization [ 116 ]. Lima and collaborators (2015) used rat models of 5-FU-induced OM to test AM or not, as a natural dressing biomaterial, during 14 or 21 days [ 116 ]. Immunostaining for PCNA and VEGF revealed increased cellular proliferation and neovascularization in the 5-FU-induced OM group treated with AM at days three and seven, the inflammatory and proliferative phases, respectively [ 116 ].…”

Section: Introductionmentioning

confidence: 99%

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Emerging approaches of wound healing in experimental models of high-grade oral mucositis induced by anticancer therapy

et al. 2021

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Clinical guidelines for oral mucositis (OM) still consist in palliative care. Herein, we summarize cellular and molecular mechanisms of OM ulceration in response to chemical therapies in animal models. We discuss evidenced anti-inflammatory and anti-oxidant drugs which have not been ever used for OM, such as synthetic peptides as well as cell therapy with mesenchymal stem cells; amniotic membranes, mucoadhesive polymers loaded with anti-inflammatory agents and natural or synthetic electrospun. These approaches have been promising to allow the production of drugloaded membranes, scaffolds for cells encapsulation or guided tissue regeneration.

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Amniotic membrane as a biological dressing for 5-fluoruracil-induced oral mucositis in rats

Cited by 10 publications

References 14 publications

Periodontal Diseases in Patients with Special Health Care Needs

Periodontal Diseases in Patients with Special Health Care Needs

Anti-inflammatory and healing action of oral gel containing borneol monoterpene in chemotherapy-induced mucositis in rats ( Rattus norvegicus )

Emerging approaches of wound healing in experimental models of high-grade oral mucositis induced by anticancer therapy

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