Amniotic fluid phospholipid patterns in normal and abnormal pregnancies

Fetal Pulmonary Surfactant in Amniotic Fluid of Ewes

“…Nelson (1969) analyzed lipids in human amniotic fluids from normal and abnormal pregnancies. His data indicated the most abundant phospholipid to be phospha tidylcholine.…”

Effects of Gestational Age, Dexamethasone, and Metopirone on Lecithin Concentration in Fetal Lung Tissue and Amniotic Fluid in Rats and Guinea Pigs

“…The procedure for analysis of lung and amniotic fluid lecithin phosphorus is essentially the same as that previously described for placenta [4] and amniotic fluid [5] except the lung tissue was homogenized in a Virtis blender three times and re covery was determined for both lung tissue and amniotic fluid by adding radio active lecithin to the original tissue or fluid and counting a portion of the reconstituted residue after extraction and evaporation. These recovery data were used in the final calculations.…”

Amniotic Fluid Surface Tension during Pregnancy

“…The concentration of the amniotic fluid lecithin was determined following thin-layer chromatographic separation according to Nelson (2,3). The lecithin level is reported as mg% of lecithin phosphorus.…”

“…The surface tension of the amniotic fluid in relation to gestational age has received little attention since the possibility of predicting foetal pulmonary maturity was obtained with the lecithin/sphingomyelin ratio (1) and with sur face active lecithin determination in the amniotic fluid (2,3). A test based on the indirect measure of the surface tension was devised by Clements et al (4) and has proved to be very useful in predicting foetal plumonary maturity.…”