Amniotic fluid metabolomic and lipidomic alterations associated with hemoglobin Bart’s diseases

Chen, Xiaohang; Chen, Hongyan; Nie, Haimei; Li, Gaochi; Su, Jinjiang; Cao, Xianzhen; Cao, Yongli; Wei, Fengxiang

doi:10.1007/s11306-021-01834-y

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…Urine could be rapidly and easily obtained by the patients in a noninvasive manner. Moreover, the metabolite components and concentrations in urine are good indicators of metabolic fluctuations [8][9][10][11][12]. Thus, urinary metabolomic markers could collaborate to establish a more efficacious, cheap, and safe screening method for diagnosis of many diseases.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, urine is an optimal specimen to develop biomarkers to diagnose and monitor DAI survival noninvasively. Metabolomics, which focuses on providing an unbiased view of changes in endogenous metabolites, has been successfully applied for identifying diagnostic biomarkers of many diseases [9][10][11][12]. At present, one of the most powerful analytical technologies for nontargeted metabonomic mapping is ultraperformance liquid chromatography quadrupole-time-of-flight hybrid mass spectrometry (UPLC/Q-TOF MS) technology, which could accurately quantify and discover the remarkably altered metabolites in biofluids or tissues.…”

Section: Introductionmentioning

confidence: 99%

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UPLC/Q-TOF MS-Based Urine Metabonomics Study to Identify Diffuse Axonal Injury Biomarkers in Rat

et al. 2022

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Diffuse axonal injury (DAI) represents a frequent traumatic brain injury (TBI) type, significantly contributing to the dismal neurological prognosis and high mortality in TBI patients. The increase in mortality can be associated with delayed and nonspecific initial symptoms in DAI patients. Additionally, the existing approaches for diagnosis and monitoring are either low sensitivity or high cost. Therefore, novel, reliable, and objective diagnostic markers should be developed to diagnose and monitor DAI prognosis. Urine is an optimal sample to detect biomarkers for DAI noninvasively. Therefore, the DAI rat model was established in this work. Meanwhile, the ultraperformance liquid chromatography quadrupole-time-of-flight hybrid mass spectrometry- (UPLC/Q-TOF MS-) untargeted metabolomics approach was utilized to identify the features of urine metabolomics to diagnose DAI. This work included 57 metabolites with significant alterations and 21 abnormal metabolic pathways from the injury groups. Three metabolites, viz., urea, butyric acid, and taurine, were identified as possible biomarkers to diagnose DAI based on the great fold changes (FCs) and biological functions during DAI. The present study detected several novel biomarkers for noninvasively diagnosing and monitoring DAI and helped understand the DAI-associated metabolic events.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

UPLC/Q-TOF MS-Based Urine Metabonomics Study to Identify Diffuse Axonal Injury Biomarkers in Rat

et al. 2022

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Kidney and plasma metabolomics provide insights into the molecular mechanisms of urate nephropathy in a mouse model of hyperuricemia

Zhang

Yan

et al. 2022

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

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Cyclophosphamide Induces Lipid and Metabolite Perturbation in Amniotic Fluid during Rat Embryonic Development

et al. 2022

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Cyclophosphamide (CP) has been proven to be an embryo-fetal toxic. However, the mechanism responsible for the toxicity of the teratogenic agent has not been fully explored. This study aimed to examine the teratogenicity of CP when administered in the sensitive period of pregnant rats. The effect of CP on the lipid and metabolic profiles of amniotic fluid was evaluated using a UHPLC-Q-Exactive Orbitrap MS-based method. Metabolome analysis was performed using the MS-DIAL software with LipidBlast and NIST. Initially, we identified 636 and 154 lipid compounds in the positive and negative ion modes and 118 metabolites for differential analysis. Mainly 4 types of oxidized lipids in the amniotic fluid were found to accumulate most significantly after CP treatment, including very-long-chain unsaturated fatty acids (VLCUFAs), polyunsaturated fatty acid (PUFA)-containing triglycerides (TGs), oxidized phosphatidylcholine (PC), and sphingomyelin (SM). Tryptophan and some long-chain saturated fatty acids were lowered pronouncedly after CP treatment. These findings suggest that CP may exert teratogenic toxicity on pregnant rats through maternal and fetal oxidative stress. The UHPLC-Q-Exactive Orbitrap MS-based lipidomics approach is worthy of wider application for evaluating the potential toxicity of other agents (toxicants) during embryonic development.

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Amniotic fluid metabolomic and lipidomic alterations associated with hemoglobin Bart’s diseases

Cited by 3 publications

References 29 publications

UPLC/Q-TOF MS-Based Urine Metabonomics Study to Identify Diffuse Axonal Injury Biomarkers in Rat

UPLC/Q-TOF MS-Based Urine Metabonomics Study to Identify Diffuse Axonal Injury Biomarkers in Rat

Kidney and plasma metabolomics provide insights into the molecular mechanisms of urate nephropathy in a mouse model of hyperuricemia

Cyclophosphamide Induces Lipid and Metabolite Perturbation in Amniotic Fluid during Rat Embryonic Development

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