Amniotic fluid bubble stability test as a screening procedure for predicting the risk of neonatal respiratory distress

Sproule, W. B.; Greene, M.E.; Whitfield, C. R.

doi:10.1016/0002-9378(74)90127-6

Cited by 13 publications

(1 citation statement)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results were similar to that of a previous publication [12] confirming that the "shake test" is a good screening method, since a positive result practicaUy indicates maturity of the fetal lung. However, the high percentage (30%) of false negatives (samples with negative "shake test" and L/S ratio greater than 2.0) indicates that the "shake test" may not be safe, speciaJIy in high risk pregnancies where a clinical confirmation should be reached soonest.…”

Section: Commentssupporting

confidence: 90%

Determination of fetal lung maturity by L/S ratio, “shake test” and phosphatidylglycerol in amniotic fluid.

Bustos¹,

Giussi²,

Vinacur³

et al. 1979

Journal of Perinatal Medicine

View full text Add to dashboard Cite

Section: Commentssupporting

confidence: 90%

Determination of fetal lung maturity by L/S ratio, “shake test” and phosphatidylglycerol in amniotic fluid.

Bustos¹,

Giussi²,

Vinacur³

et al. 1979

Journal of Perinatal Medicine

View full text Add to dashboard Cite

Lecithin/sphingomyelin Ratio versus Rapid Surfactant Test in Normal and Diabetic Pregnancies

Шарф¹,

Elrad²,

Boxer³

et al. 1976

International Journal of Gynecology & Obstetrics

View full text Add to dashboard Cite

Lecithin/sphingomyelin (L/S) ratio was measured and rapid surfactant test (RST) was performed on amniotic fluid samples drawn from 60 normal and 18 diabetic pregnancies, for determination of fetal lung maturity. The results were compared to the neonatal outcome. Comparison of L/S ratio and RST demonstrates a good correlation (95%) between the two tests in normal pregnancies. In diabetic pregnancies, however, correlation is notably lower (54%). Respiratory distress syndrome (RDS) was predicted in both L/S ratio and RST in 35-40% of normal pregnancies, with 60-65% of false negative results. In diabetic pregnancies the L/S ratio was as reliable as in normal pregnancies, but the RST was less reliable, with an RDS prediction rate of 18%. The assumption is made that increased amniotic fluid volume in diabetic pregnancies, results in dilution of the surfactant giving more false negative results in RST than are seen with L/S ratio, as in the latter, by measuring the ratio between the two constituents of amniotic fluid, the dilution factor is abolished. In diabetic pregnancies, a positive RST is reliable, but with negative results, L/S ratio must be determined for correct assessment of fetal lung maturity.

show abstract

High pressure liquid chromatography of standard and amniotic fluid phospholipids

Hsieh¹,

Turcotte

Waraska

et al. 1980

J. High Resol. Chromatogr.

View full text Add to dashboard Cite

A method for the HPLC separation of phosphatidylglycerol (PG), phosphatidylinositol (PI), phosphatidylcholine (PC), and sphingornyelin (SPH) was achieved using five in-series columns packed with LiChrosorb, Partisil, and p-Porasil adsorbents, a solvent mixture of chloroform/methanol/ammonium hydroxide (50 : 36 : 6.7, by volume), and a Pye LCM2 Moving Wire (FID) detector. The same phospholipid mixture was also separated using four p-Porasil columns with the same eluent and detector. The latter conditions were found to be suitable for the analysis of phospholipids obtained after centrifuging, extraction, and precipitation of surface-active lipid components of patient amniotic fluid collected at amniocentesis section. The lecithin/ sphingomyelin (US) ratios, determined by the HPLC method, correlated well with those determined by the TLC technique in four normal pregnancies, whereas results of shake tests did not correlate too well with L/S ratios determined by the above two chromatographic methods. Besides the lecithin/ sphingomyelin ratio, the present method was able to supply additional information: the concentrations of phosphatidylglycerol and phosphatidylinositol, for prediction of fetal lung maturity, and the palmitic acid content of amniotic fluid phosphatidyicholine. 0344-71 38/80/080400-05$01 .OO 0 1980 Dr. Alfred Huethig Publishers

show abstract

Amniotic fluid bubble stability test as a screening procedure for predicting the risk of neonatal respiratory distress

Cited by 13 publications

References 8 publications

Determination of fetal lung maturity by L/S ratio, “shake test” and phosphatidylglycerol in amniotic fluid.

Determination of fetal lung maturity by L/S ratio, “shake test” and phosphatidylglycerol in amniotic fluid.

Lecithin/sphingomyelin Ratio versus Rapid Surfactant Test in Normal and Diabetic Pregnancies

High pressure liquid chromatography of standard and amniotic fluid phospholipids

Contact Info

Product

Resources

About