Amniotic Fluid and Maternal Serum Metabolic Signatures in the Second Trimester Associated with Preterm Delivery

Virgiliou, Christina; Gika, Helen; Witting, Michael; Bletsou, Anna A.; Athanasiadis, Apostolos; Zafrakas, Μenelaos; Thomaidis, Nikolaos S.; Raikos, Νikolaos; Makrydimas, G.; Theodoridis, Georgios

doi:10.1021/acs.jproteome.6b00845

Cited by 54 publications

(89 citation statements)

References 49 publications

(73 reference statements)

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“…As these metabolites exhibit a wide variety of physicochemical properties, a generic extraction procedure is typically applied for LC-MS based metabolomics. Here the simple protein precipitation method often applied in our studies [31], and three alternate methods were compared. Figure 1 depicts schematically the standard PPT and the tree tested procedures.…”

Section: Resultsmentioning

confidence: 99%

Sample Preparation Strategies for the Effective Quantitation of Hydrophilic Metabolites in Serum by Multi-Targeted HILIC-MS/MS

et al. 2017

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The effect of endogenous interferences of serum in multi-targeted metabolite profiling HILIC-MS/MS analysis was investigated by studying different sample preparation procedures. A modified QuEChERS dispersive SPE protocol, a HybridSPE protocol, and a combination of liquid extraction with protein precipitation were compared to a simple protein precipitation. Evaluation of extraction efficiency and sample clean-up was performed for all methods. SPE sorbent materials tested were found to retain hydrophilic analytes together with endogenous interferences, thus additional elution steps were needed. Liquid extraction was not shown to minimise matrix effects. In general, it was observed that a balance should be reached in terms of recovery, efficient clean-up, and sample treatment time when a wide range of metabolites are analysed. A quick step for removing phospholipids prior to the determination of hydrophilic endogenous metabolites is required, however, based on the results from the applied methods, further studies are needed to achieve high recoveries for all metabolites.

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Section: Resultsmentioning

confidence: 99%

Sample Preparation Strategies for the Effective Quantitation of Hydrophilic Metabolites in Serum by Multi-Targeted HILIC-MS/MS

et al. 2017

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“…86,87 As mentioned, metabolic profiles can be generated from a variety of biological fluids; this methodologic advantage was recently utilized to demonstrate biomarkers present in amniotic fluid and maternal serum collected during women's second trimester that predicted later preterm delivery. 88 Metabolomics has also been used to identify biomarkers produced in mice uterine tissue following pharmacologic stimulation versus inhibition of contractions, revealing new detail on the mechanisms of uterine contractility in rodents. 89 Our study has several strengths.…”

Section: Discussionmentioning

confidence: 99%

Metabolomic Pathways Predicting Labor Dystocia by Maternal Body Mass Index

Carlson

Frediani

Corwin

et al. 2020

AJP Rep

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Objectives The purpose of this study was to evaluate the metabolic pathways activated in the serum of African-American women during late pregnancy that predicted term labor dystocia. Study Design Matched case–control study (n = 97; 48 cases of term labor dystocia and 49 normal labor progression controls) with selection based on body mass index (BMI) at hospital admission and maternal age. Late pregnancy serum samples were analyzed using ultra-high-resolution metabolomics. Differentially expressed metabolic features and pathways between cases experiencing term labor dystocia and normal labor controls were evaluated in the total sample, among women who were obese at the time of labor (BMI ≥ 30 kg/m2), and among women who were not obese. Results Labor dystocia was predicted by different metabolic pathways in late pregnancy serum among obese (androgen/estrogen biosynthesis) versus nonobese African-American women (fatty acid activation, steroid hormone biosynthesis, bile acid biosynthesis, glycosphingolipid metabolism). After adjusting for maternal BMI and age in the total sample, labor dystocia was predicted by tryptophan metabolic pathways in addition to C21 steroid hormone, glycosphingolipid, and androgen/estrogen metabolism. Conclusion Metabolic pathways consistent with lipotoxicity, steroid hormone production, and tryptophan metabolism in late pregnancy serum were significantly associated with term labor dystocia in African-American women.

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“…Randomized trials of total LCPUFA supplementation have been conducted and found a reduction in early PTB (<34 weeks) 10,12,13 . The secondtrimester serum lipid profiling of 35 PTB women and 35 matched term delivery controls were carried out, and increased levels of lipids and changes of key end-point metabolites were found in PTB women 14 . All these discoveries imply that lipids could play an important role in PTB and could be used as potential biomarkers for PTB or interventions to extend the length of pregnancy.…”

Section: Background and Summarymentioning

confidence: 99%

Generation and quality control of maternal plasma lipidomics data associated with preterm birth

Zhong

Huang

et al. 2019

Preprint

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Preterm birth is not only one of the most common causes of infant deaths but also a great risk for them to have severe subsequent health problems. The causes of preterm birth may be due to a combination of genetic and environmental factors, however, it remains largely unknown. Here we report an untargeted lipidomics dataset of plasma specimens from 258 pregnant women at the stage of twelve to twenty-five gestational weeks. Among them, 44 had extremely to very preterm births, 54 had moderate preterm births, 71 had late preterm births and 89 had full-term deliveries. The metabolomic profiling was generated with an UPLC-MS in both the positive and negative mode, and putative identification of all the metabolites was provided by searching against online databases. The quality assessment performed on quality control samples showed that the data is reproducible, robust and reliable. Both the raw data files, the raw and processed data matrix were available on MetaboLights, which may be used as a valuable validation dataset for new findings and a test dataset for novel algorithms.

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Amniotic Fluid and Maternal Serum Metabolic Signatures in the Second Trimester Associated with Preterm Delivery

Cited by 54 publications

References 49 publications

Sample Preparation Strategies for the Effective Quantitation of Hydrophilic Metabolites in Serum by Multi-Targeted HILIC-MS/MS

Sample Preparation Strategies for the Effective Quantitation of Hydrophilic Metabolites in Serum by Multi-Targeted HILIC-MS/MS

Metabolomic Pathways Predicting Labor Dystocia by Maternal Body Mass Index

Generation and quality control of maternal plasma lipidomics data associated with preterm birth

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