Amniotic Fluid and Bone Marrow Derived Mesenchymal Stem Cells Can be Converted to Smooth Muscle Cells in the Cryo-Injured Rat Bladder and Prevent Compensatory Hypertrophy of Surviving Smooth Muscle Cells

Coppi, Paolo De; Callegari, A.; Chiavegato, Angela; Gasparotto, Lisa; Piccoli, Martina; Taiani, Jenny; Pozzobon, Michela; Boldrin, Luisa; Okabe, Masaru; Cozzi, Emanuele; Atala, Anthony; Gamba, Piergiorgio; Sartore, Saverio

doi:10.1016/j.juro.2006.09.103

Cited by 174 publications

(99 citation statements)

References 12 publications

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“…[26][27][28][29][30] Alternatively, broad potential for differentiation and high proliferation rates make AFSC well suited for use in regenerative medicine therapies such as engineered skeletal/cardiac muscles, tendons, heart valves, and blood vessels. 11,17,[31][32][33][34][35] This study confirms our previous work on the capacity for VEGF-mediated differentiation of c-kit + AFSC into endothelial-like cells, 14 as well as illustrates the vasculogenic and perivasculogenic potential of AFSC within a threedimensional fibrin/PEG scaffold in vitro.…”

Section: Discussionsupporting

confidence: 86%

Capillary-Like Network Formation by Human Amniotic Fluid-Derived Stem Cells Within Fibrin/Poly(Ethylene Glycol) Hydrogels

Benavides

Quinn

Pok

et al. 2015

Tissue Engineering Part A

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A major limitation in tissue engineering strategies for congenital birth defects is the inability to provide a significant source of oxygen, nutrient, and waste transport in an avascular scaffold. Successful vascularization requires a reliable method to generate vascular cells and a scaffold capable of supporting vessel formation. The broad potential for differentiation, high proliferation rates, and autologous availability for neonatal surgeries make amniotic fluid-derived stem cells (AFSC) well suited for regenerative medicine strategies. AFSC-derived endothelial cells (AFSC-EC) express key proteins and functional phenotypes associated with endothelial cells. Fibrin-based hydrogels were shown to stimulate AFSC-derived network formation in vitro but were limited by rapid degradation. Incorporation of poly(ethylene glycol) (PEG) provided mechanical stability (65% -9% weight retention vs. 0% for fibrin-only at day 14) while retaining key benefits of fibrin-based scaffolds-quick formation (10 -3 s), biocompatibility (88% -5% viability), and vasculogenic stimulation. To determine the feasibility of AFSC-derived microvasculature, we compared AFSC-EC as a vascular cell source and AFSC as a perivascular cell source to established sources of these cell types-human umbilical vein endothelial cells (HUVEC) and mesenchymal stem cells (MSC), respectively. Cocultures were seeded at a 4:1 endothelial-toperivascular cell ratio, and gels were incubated at 37°C for 2 weeks. Mechanical testing was performed using a stress-controlled rheometer (G¢ = 95 -10 Pa), and cell-seeded hydrogels were assessed based on morphology. Network formation was analyzed based on key parameters such as vessel thickness, length, and area, as well as the degree of branching. There was no statistical difference between individual cultures of AFSC-EC and HUVEC in regard to these parameters, suggesting the vasculogenic potential of AFSC-EC; however, the development of robust vessels required the presence of both an endothelial and a perivascular cell source and was seen in AFSC cocultures (70% -20% vessel length, 90% -10% vessel area, and 105% -10% vessel thickness compared to HUVEC/MSC). At a fixed seeding density, the coculture of AFSC with AFSC-EC resulted in a synergistic effect on network parameters similar to MSC (150% vessel length, 147% vessel area, 150% vessel thickness, and 155% branching). These results suggest that AFSC-EC and AFSC have significant vasculogenic and perivasculogenic potential, respectively, and are suited for in vivo evaluation.

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Section: Discussionsupporting

confidence: 86%

Capillary-Like Network Formation by Human Amniotic Fluid-Derived Stem Cells Within Fibrin/Poly(Ethylene Glycol) Hydrogels

Benavides

Quinn

Pok

et al. 2015

Tissue Engineering Part A

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“…Huang et al transplanted adipose-derived stem cells by intrabladder or intravenous injection resulting in improved tissue parameters and urodynamics in a rat model of overactive bladder [55]. Interestingly, De Coppi et al showed that intrabladder transplantation of amniotic fluid or bone marrow stem cells promoted post-injury bladder remodeling by a paracrine mechanism [56]. According to Hallman et al, the repair of injured renal epithelium is thought to be mediated by surviving renal proximal tubular cells that must dedifferentiate to allow for proliferation and migration necessary for epithelial regeneration.…”

Section: Bladder Dysfunction and Glomerular Injurymentioning

confidence: 99%

Perspectives of purinergic signaling in stem cell differentiation and tissue regeneration

Glaser

Cappellari

Pillat

et al. 2011

Purinergic Signalling

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Replacement of lost or dysfunctional tissues by stem cells has recently raised many investigations on therapeutic applications. Purinergic signaling has been shown to regulate proliferation, differentiation, cell death, and successful engraftment of stem cells originated from diverse origins. Adenosine triphosphate release occurs in a controlled way by exocytosis, transporters, and lysosomes or in large amounts from damaged cells, which is then subsequently degraded into adenosine. Paracrine and autocrine mechanisms induced by immune responses present critical factors for the success of stem cell therapy. While P1 receptors generally exert beneficial effects including anti-inflammatory activity, P2 receptor-mediated actions depend on the subtype of stimulated receptors and localization of tissue repair. Pro-inflammatory actions and excitatory tissue damages mainly result from P2X7 receptor activation, while other purinergic receptor subtypes participate in proliferation and differentiation, thereby providing adequate niches for stem cell engraftment and novel mechanisms for cell therapy and endogenous tissue repair. Therapeutic applications based on regulation of purinergic signaling are foreseen for kidney and heart muscle regeneration, Clara-like cell replacement for pulmonary and bronchial epithelial cells as well as for induction of neurogenesis in case of neurodegenerative diseases.

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“…Amniotic fluid stem (AFS) cells are broadly multipotent [1] and are able to repopulate the bone marrow (BM) when transplanted into immunocompromised animals [3], while differing from pluripotent stem cells because of their inability to form teratomas [1]. AFS cells have been tested in various disease models and have been shown to contribute to lung [4], kidney [5,6], cardiac [7], and smooth muscle regeneration [8]. They can also generate myotubes in vitro [1], but very little is known about their potential to functionally engraft into regenerating skeletal muscle [9].…”

Section: Introductionmentioning

confidence: 99%

Amniotic Fluid Stem Cells Restore the Muscle Cell Niche in a HSA‐Cre , Smn ^F7/F7 Mouse Model

et al. 2012

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Mutations in the survival of motor neuron gene (SMN1) are responsible for spinal muscular atrophy, a fatal neuromuscular disorder. Mice carrying a homozygous deletion of Smn exon 7 directed to skeletal muscle (HSA-Cre, Smn F7/F7 mice) present clinical features of human muscular dystrophies for which new therapeutic approaches are highly warranted. Herein we demonstrate that tail vein transplantation of mouse amniotic fluid stem (AFS) cells enhances the muscle strength and improves the survival rate of the affected animals. Second, after cardiotoxin injury of the Tibialis Anterior, only AFS-transplanted mice efficiently regenerate. Most importantly, secondary transplants of satellite cells (SCs) derived from treated mice show that AFS cells integrate into the muscle stem cell compartment and have long-term muscle regeneration capacity indistinguishable from that of wild-type-derived SC. This is the first study demonstrating the functional and stable integration of AFS cells into the skeletal muscle, highlighting their value as cell source for the treatment of muscular dystrophies.

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Amniotic Fluid and Bone Marrow Derived Mesenchymal Stem Cells Can be Converted to Smooth Muscle Cells in the Cryo-Injured Rat Bladder and Prevent Compensatory Hypertrophy of Surviving Smooth Muscle Cells

Abstract: In this model stem cell transplantation has a limited effect on smooth muscle cell regeneration. Instead it can regulate post-injury bladder remodeling, possibly via a paracrine mechanism.

Cited by 174 publications

References 12 publications

Capillary-Like Network Formation by Human Amniotic Fluid-Derived Stem Cells Within Fibrin/Poly(Ethylene Glycol) Hydrogels

Capillary-Like Network Formation by Human Amniotic Fluid-Derived Stem Cells Within Fibrin/Poly(Ethylene Glycol) Hydrogels

Perspectives of purinergic signaling in stem cell differentiation and tissue regeneration

Amniotic Fluid Stem Cells Restore the Muscle Cell Niche in a HSA‐Cre , Smn ^F7/F7 Mouse Model

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Amniotic Fluid and Bone Marrow Derived Mesenchymal Stem Cells Can be Converted to Smooth Muscle Cells in the Cryo-Injured Rat Bladder and Prevent Compensatory Hypertrophy of Surviving Smooth Muscle Cells

Abstract: In this model stem cell transplantation has a limited effect on smooth muscle cell regeneration. Instead it can regulate post-injury bladder remodeling, possibly via a paracrine mechanism.

Cited by 174 publications

References 12 publications

Capillary-Like Network Formation by Human Amniotic Fluid-Derived Stem Cells Within Fibrin/Poly(Ethylene Glycol) Hydrogels

Capillary-Like Network Formation by Human Amniotic Fluid-Derived Stem Cells Within Fibrin/Poly(Ethylene Glycol) Hydrogels

Perspectives of purinergic signaling in stem cell differentiation and tissue regeneration

Amniotic Fluid Stem Cells Restore the Muscle Cell Niche in a HSA‐Cre , Smn F7/F7 Mouse Model

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Product

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About

Amniotic Fluid Stem Cells Restore the Muscle Cell Niche in a HSA‐Cre , Smn ^F7/F7 Mouse Model