Amniotic epithelial stem cell biocompatibility for electrospun poly(lactide- co -glycolide), poly(ε-caprolactone), poly(lactic acid) scaffolds

Russo, Valentina; Tammaro, Loredana; Marcantonio, Lisa Di; Sorrentino, Andrea; Ancora, Massimo; Valbonetti, Luca; Turriani, Maura; Martelli, Alessandra; Cammà, Cesare; Barboni, Barbara

doi:10.1016/j.msec.2016.06.092

Cited by 29 publications

(26 citation statements)

References 44 publications

(61 reference statements)

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“…We next sought to demonstrate the biocompatibility of PPD‐E hydrogels to epidermal cells by way of a fluorescence microscopic observation analysis. To visualize observed cells survival after contacting with hydrogels, two supravital dyes were employed (PKH 26, as the red fluorescence dye links to cell membrane, and Hoechst 33342, as the blue fluorescence dye that specifically binds to DNA) . As shown in Figure c, PKH 26/Hoechst 33342‐labeled cells (merged images) exhibited significant increase over time for both PPD hydrogels and control group.…”

Section: Resultsmentioning

confidence: 99%

A Biomimetic Mussel‐Inspired ε‐Poly‐l‐lysine Hydrogel with Robust Tissue‐Anchor and Anti‐Infection Capacity

Wang

Chen

et al. 2017

Adv Funct Materials

383

336

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In situ hydrogels have attracted considerable attention in tissue engineering because of their minimal invasiveness and ability to match the irregular tissue defects. However, hydrous physiological environments and the high level of moisture in hydrogels severely hamper binding to the target tissue and easily cause wound infection, thereby limiting the effectiveness in wound care management. Thus, forming an intimate assembly of the hydrogel to the tissue and preventing wound infecting still remains a significant challenge. In this study, inspired by mussel adhesive protein, a biomimetic dopamine‐modified ε‐poly‐l‐lysine‐polyethylene glycol‐based hydrogel (PPD hydrogel) wound dressing is developed in situ using horseradish peroxidase cross‐linking. The biomimetic catechol–Lys residue distribution in PPD polymer provides a catechol–Lys cooperation effect, which endows the PPD hydrogels with superior wet tissue adhesion properties. It is demonstrated that the PPD hydrogel can facilely and intimately integrate with biological tissue and exhibits superior capacity of in vivo hemostatic and accelerated wound repair. In addition, the hydrogels exhibit outstanding anti‐infection property because of the inherent antibacterial ability of ε‐poly‐l‐lysine. These findings shed new light on the development of mussel‐inspired tissue‐anchored and antibacterial hydrogel materials serving as wound dressings.

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Section: Resultsmentioning

confidence: 99%

A Biomimetic Mussel‐Inspired ε‐Poly‐l‐lysine Hydrogel with Robust Tissue‐Anchor and Anti‐Infection Capacity

Wang

Chen

et al. 2017

Adv Funct Materials

383

336

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“…Observations of wettability for electrospun nonwovens before crosslinking (Figure 8). • PLA is a polyester with contact angle values around 70°-80° for film samples, and contact angle values increase in nonwoven form, which is a known phenomenon [35] • Nonwovens consisting of PLA and LprePGS are hydrophilic owing to LprePGS, which is a hydrophilic polymer thanks to the -OH groups. The more LprePGS, the more apparent the hydrophilic nature.…”

Section: Wettabilitymentioning

confidence: 99%

“…A slightly lower contact angle for the PLA2575Hpre sample (but still in the hydrophobic range) may be because of the higher content of HprePGS, as well as (or even more likely) because of the different morphology of nonwoven compared with samples with a lower HprePGS content. • PLA is a polyester with contact angle values around 70 • -80 • for film samples, and contact angle values increase in nonwoven form, which is a known phenomenon [35] • Nonwovens consisting of PLA and LprePGS are hydrophilic owing to LprePGS, which is a hydrophilic polymer thanks to the -OH groups. The more LprePGS, the more apparent the hydrophilic nature.…”

Section: Wettabilitymentioning

confidence: 99%

Poly(Glycerol Sebacate)–Poly(l-Lactide) Nonwovens. Towards Attractive Electrospun Material for Tissue Engineering

2019

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Two types of poly(glycerol sebacate) (PGS) prepolymers were synthesized and electrospun with poly(l-lactic acid) (PLA), resulting in bicomponent nonwovens. The obtained materials were pre-heated in a vacuum, at different times, to crosslink PGS and investigate morphological and structural dependencies in that polymeric, electrospun system. As both PGS and PLA are sensitive to pre-heating (crosslinking) conditions, research concerns both components. More interest is focused on the properties of PGS, considering further research for mechanical properties and subsequent experiments with PGS synthesis. Electrospinning of PGS blended with PLA does not bring difficulties, but obtaining elastomeric properties of nonwovens is problematic. Even though PGS has many potential advantages over other polyesters when soft tissue engineering is considered, its full utilization via the electrospinning process is much harder in practice. Further investigations are ongoing, especially with the promising PGS prepolymer with a higher esterification degree and its variations.

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“…Recently, it has been demonstrated that ovine AECs (oAECs) are biocompatible for polymeric fleeces made from polymers as poly-ε-caprolactone (PCL), polylactide (PLA), and poly(lactide-coglycolide) PLGA copolymers. In particular, they are highly biocompatible for PLGA fleeces with encouraging results in terms of cell colonization, distribution, and proliferation within the construct [38]. Contextually, PLGA, when electrospun, becomes a fibrous device.…”

Section: Introductionmentioning

confidence: 99%

Tendon Biomimetic Electrospun PLGA Fleeces Induce an Early Epithelial-Mesenchymal Transition and Tenogenic Differentiation on Amniotic Epithelial Stem Cells

Russo

Khatib

Marcantonio

et al. 2020

Cells

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Background. The design of tendon biomimetic electrospun fleece with Amniotic Epithelial Stem Cells (AECs) that have shown a high tenogenic attitude may represent an alternative strategy to overcome the unsatisfactory results of conventional treatments in tendon regeneration. Methods. In this study, we evaluated AEC-engineered electrospun poly(lactide-co-glycolide) (PLGA) fleeces with highly aligned fibers (ha-PLGA) that mimic tendon extracellular matrix, their biocompatibility, and differentiation towards the tenogenic lineage. PLGA fleeces with randomly distributed fibers (rd-PLGA) were generated as control. Results. Optimal cell infiltration and biocompatibility with both PLGA fleeces were shown. However, only ha-PLGA fleeces committed AECs towards an Epithelial-Mesenchymal Transition (EMT) after 48 h culture, inducing their cellular elongation along the fibers’ axis and the upregulation of mesenchymal markers. AECs further differentiated towards tenogenic lineage as confirmed by the up-regulation of tendon-related genes and Collagen Type 1 (COL1) protein expression that, after 28 days culture, appeared extracellularly distributed along the direction of ha-PLGA fibers. Moreover, long-term co-cultures of AEC-ha-PLGA bio-hybrids with fetal tendon explants significantly accelerated of half time AEC tenogenic differentiation compared to ha-PLGA fleeces cultured only with AECs. Conclusions. The fabricated tendon biomimetic ha-PLGA fleeces induce AEC tenogenesis through an early EMT, providing a potential tendon substitute for tendon engineering research.

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Amniotic epithelial stem cell biocompatibility for electrospun poly(lactide- co -glycolide), poly(ε-caprolactone), poly(lactic acid) scaffolds

Cited by 29 publications

References 44 publications

A Biomimetic Mussel‐Inspired ε‐Poly‐l‐lysine Hydrogel with Robust Tissue‐Anchor and Anti‐Infection Capacity

A Biomimetic Mussel‐Inspired ε‐Poly‐l‐lysine Hydrogel with Robust Tissue‐Anchor and Anti‐Infection Capacity

Poly(Glycerol Sebacate)–Poly(l-Lactide) Nonwovens. Towards Attractive Electrospun Material for Tissue Engineering

Tendon Biomimetic Electrospun PLGA Fleeces Induce an Early Epithelial-Mesenchymal Transition and Tenogenic Differentiation on Amniotic Epithelial Stem Cells

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