AmnioClip-Plus as Sutureless Alternative to Amniotic Membrane Transplantation to Improve Healing of Ocular Surface Disorders

Abstract

The medicinal benefits of amniotic membrane transplantation for ocular surface disorders are well accepted worldwide. Even in high-risk keratoplasties, the concomitant use of amniotic membrane has demonstrated its value in improving graft survival. However, its seam-associated application can lead to an additional trauma. The AmnioClip ring system, into which the amniotic membrane is clamped (AmnioClip-plus, AC+), was developed to avoid this surgical intervention. The AC+ is placed on the cornea, similar to a contact lens, under local anesthesia and can therefore be applied repeatedly. Clinical practice demonstrates the easy handling, good compatibility, and efficacy of this minimally invasive method.

PURION® processed human amnion chorion membrane allografts retain material and biological properties supportive of soft tissue repair

“…However, it is also critical to understand the impact of processing techniques on the biomolecules embedded within the allografts. 20 A multiplex ELISA detected the proteins contained within LHACM and identified a variety of growth factors and cytokines that regulate vital processes in wound healing including cell migration, proliferation, inflammation, angiogenesis and ECM remodeling. 38 Similar factors have previously been identified in DHACM, a dehydrated amniotic membrane allograft.…”

“…In order to provide the most beneficial and safe amniotic membrane allograft for therapeutic purposes it is necessary to utilize the best methods for collection, processing and storage while preserving the native structure of the amniotic tissue. 20 Extensive analysis of PURION ® processed amniotic membrane allografts has demonstrated that this processing method retains well‐known regulatory proteins and preserves the bioactivity to stimulate cellular activities supportive of wound healing. Indeed, increases in proliferation and migration of multiple cell types including stem cells, HDFs, tenocytes, and endothelial cells highlights the ability of amniotic membrane allografts to impact basic cellular functions.…”

“…15,18,19 The clinical success of amniotic membrane allografts is related to the processing techniques that preserve the membrane properties, including the biophysical structure and bioactivity, while making them safe and readily accessible for the provider. 9,20…”

Dehydrated human amnion/chorion membrane allograft with spongy layer to significantly improve the outcome of chronic non‐healing wounds

“…Among these, dehydrated amnion/chorion membranes (dHACMs) are the most widely utilised for the promotion of wound healing. However, the specific structure and composition of dHACMs, including the presence of amnion or chorion only, or both, along with various matrix components, growth factors and cytokines, depend on the processing methods employed 16–22 . Generally, the amnion and chorion from donated human placentas are isolated through a cleansing proprietary process and either processed separately or laminated to form a two‐layer graft, before being dehydrated under controlled drying conditions 23 .…”

“…However, the specific structure and composition of dHACMs, including the presence of amnion or chorion only, or both, along with various matrix components, growth factors and cytokines, depend on the processing methods employed. [16][17][18][19][20][21][22] Generally, the amnion and chorion from donated human placentas are isolated through a cleansing proprietary process and either processed separately or laminated to form a two-layer graft, before being dehydrated under controlled drying conditions. 23 All of the dHACMs that have been utilised in clinical trials to date lack the spongy layer, which bridges the amnion and chorion sides and, thus, is also referred to as an intermediate layer.…”