Amniocentesis and biochemical evaluation of amniotic fluid in ewes at 70, 100 and 145 days of pregnancy

Prestes, Nereu Carlos; Chalhoub, Mcl; Lopes, Maria Denise; Takahira, Regina Kiomi

doi:10.1016/s0921-4488(00)00202-9

Cited by 22 publications

(34 citation statements)

References 14 publications

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“…Creatinine mean concentration observed in groups 1 and 2 was lower than the one found in the bovine species (BAETZ et al, 1976), but for group 3 mean concentration values were similar. Urea concentration values for group 1 were similar to the ones found for the bovine (BAETZ et al, 1976) and ovine (PRESTES et al, 2001) species. These creatinine and urea concentration values suggested satisfactory kidney maturation.…”

Section: Discussionsupporting

confidence: 83%

“…Large amount of the produced glucose is obtained via facilitated transport across the maternal and the fetal placenta. The mean concentration values of glucose in the three groups were higher than the ones found in the ovine species (PRESTES et al, 2001). The finding of no statistical difference in the results indicates that the maternal-fetal glucose transport were at normal levels.…”

Section: Discussioncontrasting

confidence: 62%

“…In relation to the mean concentration of GGT, this experiment had shown that the three groups had a higher level of this enzyme than the one reported in ovine species (PRESTES et al, 2001).…”

Section: Discussionmentioning

confidence: 52%

“…The amniotic fluid's sodium and chloride concentrations depend on the oronasal secretions during gestation (EVRARD et al, 1997). The mean sodium concentration in the amniotic fluid in the three groups was lower than other results found in the reviewed literature (BRACE et al, 2004;GAGNON et al, 2002;PRESTES et al, 2001) for the ovine species. The potassium concentration in group 1 was similar to the one described in the ovine species by Brace et al (2004), but its value was lower in group 2 and its value was higher in group 3.…”

Section: Discussionmentioning

confidence: 56%

“…Not only that, but the fluid promotes cervix, vagina and vulva dilatation during parturition, lubricating the vagina after the chorioallantois disruption helping the calf to pass through the vaginal canal. Apart from having bactericidal proprieties, these fluids also avoid skin adhesions (PRESTES; LANDIM-ALVARENGA, 2006 ;TONIOLLO;VICENTE, 1995).…”

Section: Introductionmentioning

confidence: 99%

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Biochemical and Hormonal Profile of the Amniotic Fluid From Nelore Calves Conceived by Different Reproduction Biotechnologies

Piagentini

Prestes

Moya

et al. 2012

Rev. Acad. Ciênc. Anim.

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This study aimed to quantify biochemical constituents and hormonal concentrations in the amniotic fluid of Nelore calves conceived by different reproduction biotechnologies. Sixty animals were used and divided into: 1 – Twenty Nelore cows pregnant of calves obtained by artificial insemination; 2 – Twenty cows pregnant with Nelore calves obtained by embryo transference; 3 – Twenty cows pregnant with Nelore calves obtained by in vitro production. During the expulsion phase, the amnion was punctured and 15 mL of fluid were collected. The biochemical evaluation was performed with commercial kits in accordance to the manufacturer’s recommendations. It was used the radioimmunoassay to assess the progesterone and testosterone levels. The statistical analysis used was the variance analysis and Tukey (5% significance level). Mean urea and creatinine when comparing the three groups showed that group 3 had higher values of these two metabolites, suggesting that the kidney maturity is suitable. For gamma-glutamyltransferase, group 3 had a lower value compared to group 1. This enzyme is related to metabolism, may be inferred that the metabolic activity is high in animal production in vitro. The glucose values did not differ between the groups, suggests that the maternal-fetal exchanges were suitable for the power supply to the fetus. Lower chloride concentration was showed in group 3. Sodium and potassium were found in greater quantities in group 3 when compared to other groups. There was no significant difference for the testosterone and progesterone. The results obtained in biochemical analysis can conclude that the renal and liver activity and maternal-fetal exchange are normal in the different groups.

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Section: Discussionsupporting

confidence: 83%

Section: Discussioncontrasting

confidence: 62%

Section: Discussionmentioning

confidence: 52%