Amnibacterium flavum sp. nov., a novel endophytic actinobacterium isolated from bark of Nerium indicum Mill.

Li, Tuo; Yan, Xiao-Rui; Li, Fei-Na; Yang, Chao; An, M.; Sun, Cheng‐Hang

doi:10.1099/ijsem.0.003156

Cited by 14 publications

(4 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Gram-staining test was performed using Gram-staining kit (Changde BKMAN Biotechnology Co., Ltd). The temperature ranges for growth of the two novel strains and the reference strain were tested by incubation on TSA at 4,10,15,20,25,28,30,35,37,40,45,50, 55 and 60 °C for 1 week. The pH range for growth was measured by incubation on TSA at pH 4-12 (at intervals of 1 pH unit) for 1 week.…”

Section: Morphological Physiological and Biochemical Characteristicsmentioning

confidence: 99%

“…The Microbial Identification software package (Sherlock version 6.1; midi database, TSBA6) was used to analyse fatty acid methyl esters [39]. The separation of fatty acid methyl esters was by performed using a capillary column DB-5 MS (30 m×0.25 mm i.d.×0.25 µm; Agilent Technologies), and analysed using a Trace 1310 gas chromatograph coupled with a Trace ISQ single-quadrupole mass spectrometer equipped with the Nist 08 Library software database [40].…”

Section: Chemotaxonomic Characterizationmentioning

confidence: 99%

See 1 more Smart Citation

Paenibacillus thermotolerans sp. nov., isolated from a hot spring in Yunnan Province, south-west China

Xiang,

Yin,

Zhang

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

Two Gram-positive, rod-shaped, endospore-forming strains, YIM B05601 and YIM B05602T, were isolated from soil sampled at Hamazui hot spring, Tengchong City, Yunnan Province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences suggested that the two strains fell within the genus Paenibacillus, appearing most closely related to Paenibacillus alkalitolerans YIM B00362T (96.9 % sequence similarity). Genome-based phylogenetic analysis confirmed that strains YIM B05601 and YIM B05602T formed a distinct phylogenetic cluster within the genus Paenibacillus. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values of strains YIM B05601 and YIM B05602T with the related species P. alkalitolerans YIM B00362T were within the ranges of 74.43–74.57 % and 12.1–18.5 %, respectively, which clearly indicated that strains YIM B05601, YIM B05602T represented a novel species. Strains YIM B05601 and YIM B05602T exhibited 99.6 % 16S rRNA gene sequence similarity. The ANI and dDDH values between the two strains were 99.8 and 100 %, respectively, suggesting that they belong to the same species. Optimum growth for both strains occurred at pH 7.0 and 45 °C. The diagnostic diamino acid in the cell-wall peptidoglycan of strains YIM B05601 and YIM B05602T was meso-diaminopimelic acid. MK-7 was the predominant menaquinone. The polar lipids of strain YIM B05602T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, four unidentified glycolipids, an unidentified polarlipid and phosphatidylinositol mannoside. The major fatty acids of the two stains were iso-C15 : 0 and anteiso-C15 : 0. Based on phylogenomic and phylogenetic analyses coupled with phenotypic and chemotaxonomic characterizations, strains YIM B05601 and YIM B05602T could be classified as a novel species of the genus Paenibacillus, for which the name Paenibacillus thermotolerans sp. nov. is proposed. The type strain is YIM B05602T (=CGMCC 1.60051T=KCTC 43460T=NBRC 115924T).

show abstract

Section: Morphological Physiological and Biochemical Characteristicsmentioning

confidence: 99%

Section: Chemotaxonomic Characterizationmentioning

confidence: 99%

Paenibacillus thermotolerans sp. nov., isolated from a hot spring in Yunnan Province, south-west China

Xiang,

Yin,

Zhang

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

show abstract

“…The Microbial Identi cation software package (Sherlock version 6.1; midi database, TSBA6) was used to analyze fatty acid methyl esters (Moss and Dees 1975). The separation of fatty acid methyl esters was by performed using a capillary column DB-5MS (30m×0.25mm i.d.×0.25µm; Agilent Technologies), and analysed using a Trace 1310 gas chromatograph coupled with a Trace ISQ single-quadrupole mass spectrometer equipped with the Nist 08 Library software database (Tuo et al 2019).…”

Section: Chemotaxonomic Characterizationmentioning

confidence: 99%

Roseateles cavernae sp. nov. ， a bacterium isolated from freshwater of the Old Huanglong Cave in Yunnan province, south-west China

Zhang,

Jiang,

et al. 2024

Preprint

View full text Add to dashboard Cite

A Gram-stain-negative, rod-shaped, amylolytic bacterial strain, designated as YIM B04394T, was isolated from a freshwater sample collected from the Old Huanglong Cave in Kunming, Yunnan province, south-west China. The strain was found to be aerobic, oxidase-positive and weakly catalase- positive, grew at 10–40℃ (optimum, 28°C), pH 6.0–8.0 (optimum, pH 7.0) and low NaCl concentration (up to 5.0 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM B04394T clustered with Roseateles species. The DNA-DNA and ANI relatedness values of strain YIM B04394 T with the related species R. toxinivorans DSM 16998T and R. asaccharophilus DSM 25082T were 21.50-22.9%% and 76.47-76.93%, respectively. Major fatty acids included summed feature 3 and C16:0. MK-8 was detected as the respiratory quinone and the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and an unknown phospholipid. The DNA G + C content was 67.89 mol%. Based on phylogenomic and phylogenetic analyses coupled with phenotypic and chemotaxonomic characterizations indicated that the strain represents a novel Roseateles species for which the name Roseateles cavernae sp. nov. is proposed. The type strain is YIM B04394T (= NBRC 115775T = KCTC 92438T = CCTCC AB2021502T).

show abstract

“…×0.25 µm film thickness; Agilent Technologies) was used for separation of fatty acid methyl esters. The fatty acid methyl esters were analysed by using a Trace 1310 gas chromatograph coupled with a Trace ISQ single quadrupole mass spectrometer, and the results were identified by using the Nist 08 Library software database [31].…”

Section: Full-textmentioning

confidence: 99%

Hephaestia mangrovi sp. nov., a novel endophytic bacterium isolated from Aegiceras corniculatum

Yan

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

Self Cite

View full text Add to dashboard Cite

A Gram-stain-negative, aerobic, motile, rod-shaped bacterium, designated CMS5P-6T, was isolated from a surface-sterilized bark of Aegiceras corniculatum collected from Guangxi Zhuang Autonomous Region, PR China, and investigated by a polyphasic approach to determine its taxonomic position. Strain CMS5P-6T was found to grow optimally with 0–1 % (w/v) NaCl, at 30 °C and pH 6.0–7.0. Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested. Phylogenetic analysis showed that strain CMS5P-6T showed high 16S rRNA gene sequence similarity of 96.7 % to Hephaestia caeni DSM 25527T and Sphingomonas colocasiea CC-MHH0539T. The average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity values between strain CMS5P-6T and H. caeni DSM 25527T were 78.0, 21.7 and 70.8 %, respectively. The average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity values between strain CMS5P-6T and S. colocasiea JCM 31229T were 74.0, 19.9 and 61.4 %, respectively. Phylogenomic analyses based on genome sequences showed that strain CMS5P-6T and H. caeni DSM 25527T formed a distinct cluster within the family Sphingomonadaceae and far away from S. colocasiea JCM 31229T. The DNA G+C content of strain CMS5P-6T was determined to be 65.6 mol%. The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid and ubiquinone Q-10 was identified as the respiratory lipoquinone. The polar lipids were found to comprise diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, sphingoglycolipid and two unidentified aminolipids, and the major fatty acids were identified as C18 : 1 ω7c, C19 : 0 cycloω8c and C16 : 0. On the basis of phylogenetic, genomic, chemotaxonomic and phenotypic data, strain CMS5P-6T can be concluded to represent a novel species of the genus Hephaestia , for which the name Hephaestia mangrovi sp. nov. is proposed. The type strain is CMS5P-6T (=JCM 33125T=CGMCC 1.13868T).

show abstract

Amnibacterium flavum sp. nov., a novel endophytic actinobacterium isolated from bark of Nerium indicum Mill.

Cited by 14 publications

References 20 publications

Paenibacillus thermotolerans sp. nov., isolated from a hot spring in Yunnan Province, south-west China

Paenibacillus thermotolerans sp. nov., isolated from a hot spring in Yunnan Province, south-west China

Roseateles cavernae sp. nov. ， a bacterium isolated from freshwater of the Old Huanglong Cave in Yunnan province, south-west China

Hephaestia mangrovi sp. nov., a novel endophytic bacterium isolated from Aegiceras corniculatum

Contact Info

Product

Resources

About